pCAMBIA1300-UBi-MCS-35S-NOS-EGFP vector (V017583)

LOCUS       .                      10824 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..1998
                     /label="Ubi promoter"
                     /note="maize polyubiquitin gene promoter"
     terminator      2032..2284
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2513..2537
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3837..4463
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             4895..5965
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6034..6228
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6572..6712
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6898..7486)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7576..8367)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    8792..8816
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8894..9068)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9078..9794)
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     promoter        complement(9832..10509)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10700..10721
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10736..10766
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10774..10790
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     10798..10814
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"