LOCUS       .                      10824 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..1998
                     /label="Ubi promoter"
                     /note="maize polyubiquitin gene promoter"
     terminator      2032..2284
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2513..2537
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3837..4463
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             4895..5965
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6034..6228
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6572..6712
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6898..7486)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7576..8367)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    8792..8816
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8894..9068)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9078..9794)
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     promoter        complement(9832..10509)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10700..10721
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10736..10766
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10774..10790
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     10798..10814
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aattcctgca gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat
       61 gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt
      121 atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct atagtactac
      181 aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca
      241 attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc
      301 tttttttttg caaatagctt cacctatata atacttcatc cattttatta gtacatccat
      361 ttagggttta gggttaatgg tttttataga ctaatttttt tagtacatct attttattct
      421 attttagcct ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt
      481 agatataaaa tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt
      541 aaaaaaacta aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc
      601 gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa
      661 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc
      721 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc
      781 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc
      841 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac
      901 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa
      961 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct
     1021 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt
     1081 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat
     1141 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat
     1201 cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt
     1261 tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc
     1321 gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt
     1381 cgttctagat cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat
     1441 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
     1501 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
     1561 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
     1621 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
     1681 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
     1741 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
     1801 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
     1861 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
     1921 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
     1981 ttggtgttac ttctgcaggg tacccgggga tccgagctcg gatccactag tgatcgttca
     2041 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     2101 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     2161 tttatgaggt gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     2221 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     2281 gatcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     2341 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2401 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc tagagcagct
     2461 tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga
     2521 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataacgga tatttaaaag
     2581 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     2641 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     2701 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     2761 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     2821 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     2881 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     2941 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     3001 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     3061 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     3121 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     3181 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     3241 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
     3301 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
     3361 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
     3421 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
     3481 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
     3541 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
     3601 atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa
     3661 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
     3721 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
     3781 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
     3841 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
     3901 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
     3961 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
     4021 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
     4081 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
     4141 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
     4201 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
     4261 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
     4321 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
     4381 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
     4441 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
     4501 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
     4561 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
     4621 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
     4681 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
     4741 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
     4801 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca
     4861 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
     4921 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
     4981 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     5041 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     5101 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     5161 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     5221 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
     5281 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
     5341 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
     5401 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
     5461 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
     5521 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
     5581 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
     5641 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
     5701 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
     5761 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
     5821 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
     5881 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
     5941 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
     6001 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc
     6061 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
     6121 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
     6181 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
     6241 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
     6301 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
     6361 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
     6421 tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     6481 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6541 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     6601 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     6661 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     6721 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6781 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6841 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6901 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6961 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7021 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7081 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7141 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7201 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7261 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7321 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7381 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7441 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7501 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7561 gcattctagg tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc
     7621 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
     7681 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
     7741 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
     7801 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
     7861 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
     7921 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
     7981 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
     8041 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
     8101 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
     8161 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
     8221 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
     8281 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
     8341 ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct acagtattta
     8401 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
     8461 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
     8521 atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat
     8581 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
     8641 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
     8701 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
     8761 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     8821 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg
     8881 aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat tagaaatttt
     8941 attgatagaa gtattttaca aatacaaata catactaagg gtttcttata tgctcaacac
     9001 atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca cacattatta
     9061 tggagaaact cgagtcactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
     9121 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
     9181 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     9241 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
     9301 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
     9361 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     9421 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     9481 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     9541 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
     9601 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
     9661 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
     9721 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     9781 cccttgctca ccattcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt
     9841 cctctccaaa tgaaatgaac ttccttatat agaggaaggt cttgcgaagg atagtgggat
     9901 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
     9961 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
    10021 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
    10081 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
    10141 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
    10201 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttatca
    10261 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
    10321 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
    10381 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
    10441 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
    10501 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
    10561 agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc
    10621 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    10681 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    10741 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    10801 gaaacagcta tgaccatgat tacg
//
