Basic Vector Information
- Vector Name:
- pBAD30-Kan
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4940 bp
- Type:
- Protein expression
- Replication origin:
- p15A ori
- Host:
- E. coli
- 5' Primer:
- G36430-F1：
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pBAD30-Kan vector Map
pBAD30-Kan vector Sequence
LOCUS . 4940 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS complement(99..974)
/label="araC"
/note="L-arabinose regulatory protein"
promoter 1001..1285
/label="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
RBS 1313..1335
/label="RBS"
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
misc_feature 1343..1399
/label="MCS"
/note="pUC18/19 multiple cloning site"
terminator 1602..1688
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1780..1807
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
CDS 1939..2745
/label="KanR"
/note="aminoglycoside phosphotransferase"
rep_origin 2800..3255
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin complement(4216..4761)
/label="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
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