LOCUS       .                       4940 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(99..974)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1001..1285
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     RBS             1313..1335
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1343..1399
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      1602..1688
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1780..1807
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             1939..2745
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2800..3255
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(4216..4761)
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaaata attttgttta
     1321 actttaagaa ggagatatac atgaattcga gctcggtacc cggggatcct ctagagtcga
     1381 cctgcaggca tgcaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
     1441 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
     1501 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
     1561 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     1621 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     1681 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     1741 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
     1801 gcctttttgc gtttctacaa actcttgcag ctctggcccg tgtctcaaaa tctctgatgt
     1861 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     1921 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     1981 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2041 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2101 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2161 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2221 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2281 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2341 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2401 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2461 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2521 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2581 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2641 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2701 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggct
     2761 gtcagaccaa gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat
     2821 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2881 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2941 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3001 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3061 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaacttgaa
     3121 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     3181 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3241 taacgtttac aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3421 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3481 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3541 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtc
     3601 aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc
     3661 aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc
     3721 tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt
     3781 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     3841 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc
     3901 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc gctagcggag
     3961 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     4021 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     4081 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     4141 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     4201 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     4261 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4321 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     4381 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     4441 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     4501 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     4561 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     4621 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     4681 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     4741 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctaggat
     4801 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     4861 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctg
     4921 ctcatgtttg acagcttatc
//
