pBbB8k-GFP-gshAB(thermophilus) vector (V017535)

Basic Vector Information

Vector Name:
pBbB8k-GFP-gshAB(thermophilus)
Antibiotic Resistance:
Kanamycin
Length:
6763 bp
Type:
Protein expression
Host:
Bacteria
Promoter:
T7
5' Primer:
GFP-F
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pBbB8k-GFP-gshAB(thermophilus) vector Map

pBbB8k-GFP-gshAB(thermophilus)6763 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600araCaraBAD promoterGFPuvrrnB T1 terminatorT7Te terminatorpBBR1 Replambda t0 terminatorNeoR/KanR

pBbB8k-GFP-gshAB(thermophilus) vector Sequence

LOCUS       .                       6763 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(10..885)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        912..1196
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             1246..1959
                     /label="GFPuv"
                     /note="GFP variant optimized for excitation by UV light"
     terminator      4278..4349
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4365..4392
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     CDS             complement(4462..5121)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     terminator      complement(5708..5802)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(5836..6627)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"

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