Basic Vector Information
- Vector Name:
- pBbB8k-GFP-gshAB(thermophilus)
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6763 bp
- Type:
- Protein expression
- Host:
- Bacteria
- Promoter:
- T7
- 5' Primer:
- GFP-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pBbB8k-GFP-gshAB(thermophilus) vector Map
pBbB8k-GFP-gshAB(thermophilus) vector Sequence
LOCUS . 6763 bp DNA linear UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS complement(10..885)
/label="araC"
/note="L-arabinose regulatory protein"
promoter 912..1196
/label="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 1246..1959
/label="GFPuv"
/note="GFP variant optimized for excitation by UV light"
terminator 4278..4349
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 4365..4392
/label="T7Te terminator"
/note="phage T7 early transcription terminator"
CDS complement(4462..5121)
/label="pBBR1 Rep"
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
terminator complement(5708..5802)
/label="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS complement(5836..6627)
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
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