LOCUS       .                       6763 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(10..885)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        912..1196
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             1246..1959
                     /label="GFPuv"
                     /note="GFP variant optimized for excitation by UV light"
     terminator      4278..4349
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4365..4392
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     CDS             complement(4462..5121)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     terminator      complement(5708..5802)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(5836..6627)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacgtcttat gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa
       61 ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc
      121 aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt
      181 cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg
      241 gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc
      301 aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc
      361 catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag
      421 cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg
      481 cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt
      541 ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac
      601 ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg
      661 cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac
      721 cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat
      781 aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt
      841 aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc
      901 gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt
      961 ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca
     1021 aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa
     1081 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
     1141 aagattagcg gattctacct gacgcttttt atcgcaactc tctactgttt ctccataccc
     1201 gtttttttgg gaattcaaaa gatcttttaa gaaggagata tacatatgag taaaggagaa
     1261 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     1321 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     1381 tttatttgca ctactggaaa actacctgtt ccgtggccaa cacttgtcac tactttctct
     1441 tatggtgttc aatgcttttc ccgttatccg gatcacatga aacggcatga ctttttcaag
     1501 agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggaac
     1561 tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta
     1621 aaaggtattg attttaaaga agatggaaac attctcggac acaaactgga gtacaactat
     1681 aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc
     1741 aaaattcgcc acaacattga agatggctcc gttcaactag cagaccatta tcaacaaaat
     1801 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     1861 gccctttcga aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact
     1921 gctgctggga ttacacatgg catggatgag ctctacaaag gtggaggcgg atcaggaggc
     1981 ggtggatcca tgacattaaa ccaacttctt caaaaactgg aagctaccag ccctattctc
     2041 caagctaatt ttggaatcga gcgcgagagt ctacgtgtcg ataggcaagg acaactggtg
     2101 catacacctc acccatcctg tctaggagct cgtagtttcc acccctatat tcagactgat
     2161 ttttgcgagt ttcagatgga actcatcaca ccagttgcca aatctactac tgaggctcgc
     2221 cgatttctgg gagctattac tgatgtagct ggccgctcta ttgctacaga cgaggttctc
     2281 tggcctttgt ccatgccacc tcgtctaaag gcagaggaga ttcaagttgc tcaactggaa
     2341 aatgacttcg aacgccatta tcgtaactat ttggctgaaa aatatggaac taaactacaa
     2401 gctatctcag gtatccacta taatatggaa ctgggtaaag atttagttga ggccttgttc
     2461 caagaaagtg atcagaccga tatgattgcc ttcaaaaacg ccctctatct taagctagct
     2521 cagaactact tgcgctaccg ttgggtgatt acctatctct ttggggcctc acccatcgcc
     2581 gaacaaggtt tctttgacca ggaagttcca gaacctatgc gttccttccg taacagtgac
     2641 cacggctatg tcaataagga agagattcaa gtatcctttg taagtctaga agattatgtc
     2701 tcagccattg aaacctatat cgaacaagga gatttgattg cagagaaaga attttactca
     2761 gctgttcgtt tccgtggaca aaaggttaat cgttccttcc ttgacaaggg aatcacctac
     2821 ctagagttcc gtaatttcga ccttaaccct tttgagcgta tcggtattag tcagactact
     2881 atggacactg tgcacttact cattttagcc ttcctttggc ttgatagccc tgaaaatgtc
     2941 gaccaagctc ttgcacaagg ccacgcgcta aatgagaaaa ttgccctctc tcatcctcta
     3001 gaacctctac cttcggaggc taaaactcag gacattgtaa ctgccctaga ccaactggtg
     3061 caacactttg gacttggtga ctatcatcaa gatctggtta aacaagttaa ggcagccttt
     3121 gcggatccaa atcaaacgct ctctgcccag ctcttaccct atatcaaaga caaatctcta
     3181 gccgaatttg ctttaaacaa ggctcttgcc tatcatgatt acgactggac tgcccactat
     3241 gctctcaagg gctatgaaga gatggaactc tccacccaga tgttgctctt tgatgccatc
     3301 caaaagggga ttcactttga aatattggat gagcaagatc aattcctaaa actttggcac
     3361 caagaccatg ttgaatacgt caaaaacggt aacatgacct caaaagacaa ctacgtggtt
     3421 ccccttgcta tggctaataa gaccgtaacc aagaagattc tagcagatgc tggctttcca
     3481 gttccttcag gagacgaatt taccagtctt gaggaaggac ttgcctacta ccctcttatc
     3541 aaggataagc aaattgttgt caaacccaag tcaactaact ttggtctggg aatttccatt
     3601 ttccaagaac ctgccagtct tgacaactat caaaaagccc ttgaaattgc tttcgcagaa
     3661 gatacttctg tccttgttga agaatttatt ccaggaaccg aataccgttt cttcatcttg
     3721 gatgggcgtt gtgaggctgt gcttctgcgt gtcgctgcca atgttattgg tgatggcaaa
     3781 cacaccattc gtgaactagt cgctcagaaa aatgctaatc cattgcgtgg ccgtgatcac
     3841 cggtcacctc tggaaatcat tgagctagga gacatcgaac aactaatgtt agctcaacag
     3901 ggttatacac ctgatgatat tctcccagaa ggaaaaaagg tcaatctgcg tcgtaattcc
     3961 aacatctcta caggtggtga ctctattgat gtcactgaga ccatggattc ctcttaccaa
     4021 gaattagccg cagccatggc aactagcatg ggcgcctggg cttgcggggt tgatctgata
     4081 attccagatg aaactcaaat tgccaccaag gaaaatcctc attgcacctg cattgagctc
     4141 aactttaacc cttcgatgta tatgcacacc tactgtgctg agggtcctgg ccaagctatc
     4201 actactaaaa tcctagataa actttttcca gaaatagtgg ctggtcaaac ttagctcgag
     4261 taaggatctc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     4321 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     4381 ggcctttctg cgtttatacc taggctacag ccgatagtct ggaacagcgc acttacgggt
     4441 tgctgcgcaa cccaagtgct accggcgcgg cagcgtgacc cgtgtcggcg gctccaacgg
     4501 ctcgccatcg tccagaaaac acggctcatc gggcatcggc aggcgctgct gcccgcgccg
     4561 ttcccattcc tccgtttcgg tcaaggctgg caggtctggt tccatgcccg gaatgccggg
     4621 ctggctgggc ggctcctcgc cggggccggt cggtagttgc tgctcgcccg gatacagggt
     4681 cgggatgcgg cgcaggtcgc catgccccaa cagcgattcg tcctggtcgt cgtgatcaac
     4741 caccacggcg gcactgaaca ccgacaggcg caactggtcg cggggctggc cccacgccac
     4801 gcggtcattg accacgtagg ccaacacggt gccggggccg ttgagcttca cgacggagat
     4861 ccagcgctcg gccaccaagt ccttgactgc gtattggacc gtccgcaaag aacgtccgat
     4921 gagcttggaa agtgtcttct ggctgaccac cacggcgttc tggtggccca tctgcgccac
     4981 gaggtgatgc agcagcattg ccgccgtggg tttcctcgca ataagcccgg cccacgcctc
     5041 atgcgctttg cgttccgttt gcacccagtg accgggcttg ttcttggctt gaatgccgat
     5101 ttctctggac tgcgtggcca tgcttatctc catgcggtag gggtgccgca cggttgcggc
     5161 accatgcgca atcagctgca acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa
     5221 gtggcagtca attacagatt ttctttaacc tacgcaatga gctattgcgg ggggtgccgc
     5281 aatgagctgt tgcgtacccc ccttttttaa gttgttgatt tttaagtctt tcgcatttcg
     5341 ccctatatct agttctttgg tgcccaaaga agggcacccc tgcggggttc ccccacgcct
     5401 tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc
     5461 ggccttcggc cttgcccaag gtggcgctgc ccccttggaa cccccgcact cgccgccgtg
     5521 aggctcgggg ggcaggcggg cgggcttcgc ccttcgactg cccccactcg cataggcttg
     5581 ggtcgttcca ggcgcgtcaa ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg
     5641 ccttccactt ggtgtccaac cggcaagcga agcgcgcagg ccgcaggccg gaggcactag
     5701 tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     5761 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctctcgaacc
     5821 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     5881 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     5941 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     6001 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     6061 gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag
     6121 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     6181 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     6241 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     6301 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     6361 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     6421 ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt
     6481 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     6541 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     6601 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga
     6661 tcatgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
     6721 gggcttccca accttaccag agggcgcccc agctggcaat tcc
//
