Basic Vector Information
- Vector Name:
- pBBR1MCS3-EGFP-Tet
- Antibiotic Resistance:
- Tetracycline
- Length:
- 5894 bp
- Type:
- Protein expression
- Replication origin:
- pBBR1 oriV
- Host:
- Broad host
- Copy Number:
- Low
- Promoter:
- T7
- 5' Primer:
- T7
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pBBR1MCS3-EGFP-Tet vector Map
pBBR1MCS3-EGFP-Tet vector Sequence
LOCUS . 5894 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
promoter 281..309
/label="tet promoter"
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 357..1544
/label="TcR"
/note="tetracycline efflux protein"
protein_bind 1741..1762
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1777..1807
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 1815..1831
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
primer_bind 1839..1855
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 1876..1894
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 1913..2629
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
primer_bind complement(2640..2656)
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(2689..2707)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2717..2733)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(3443..4102)
/label="pBBR1 Rep"
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
rep_origin complement(4103..4872)
/label="pBBR1 oriV"
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
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