LOCUS       .                       5894 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        281..309
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             357..1544
                     /label="TcR"
                     /note="tetracycline efflux protein"
     protein_bind    1741..1762
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1777..1807
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1815..1831
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     1839..1855
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1876..1894
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             1913..2629
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     primer_bind     complement(2640..2656)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(2689..2707)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2717..2733)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3443..4102)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(4103..4872)
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      241 gcgtatcacg aggccctttc gtcttcaaga attctcatgt ttgacagctt atcatcgata
      301 agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga
      361 aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag
      421 gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg
      481 ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg
      541 ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg
      601 gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg
      661 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      721 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
      781 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
      841 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
      901 aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct
      961 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     1021 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     1081 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     1141 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     1201 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
     1261 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     1321 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     1381 ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct
     1441 cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc
     1501 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
     1561 gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
     1621 tgtgaatgcg caaatgcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1681 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1741 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1801 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     1861 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctgggta ccatggtgag
     1921 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1981 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     2041 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     2101 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     2161 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     2221 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     2281 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     2341 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     2401 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     2461 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     2521 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2581 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aacccggggg
     2641 atccactagt tctagagcgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt
     2701 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     2761 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2821 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt
     2881 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2941 ccaataggcc gactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
     3001 gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga
     3061 aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt
     3121 atgtctattg ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct
     3181 caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga
     3241 tcatttattc tgcctcccag agcctgataa aaacggtgaa tccgttagcg aggtgccgcc
     3301 ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
     3361 gacaaggtat agggcggcga ggcggctaca gccgatagtc tggaacagcg cacttacggg
     3421 ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg
     3481 gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc
     3541 gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg
     3601 gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg
     3661 tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa
     3721 ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca
     3781 cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga
     3841 tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga
     3901 tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca
     3961 cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct
     4021 catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga
     4081 tttctctgga ctgcgtggcc atgcttatct ccatgcggta gggtgccgca cggttgcggc
     4141 accatgcgca atcagctgca acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa
     4201 gtggcagtca attacagatt ttctttaacc tacgcaatga gctattgcgg ggggtgccgc
     4261 aatgagctgt tgcgtacccc ccttttttaa gttgttgatt tttaagtctt tcgcatttcg
     4321 ccctatatct agttctttgg tgcccaaaga agggcacccc tgcggggttc ccccacgcct
     4381 tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc
     4441 ggccttcggc cttgcccaag gtggcgctgc ccccttggaa cccccgcact cgccgccgtg
     4501 aggctcgggg ggcaggcggg cgggcttcgc cttcgactgc ccccactcgc ataggcttgg
     4561 gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc
     4621 cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc
     4681 ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt ggagccggtg
     4741 ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag
     4801 gcgcagcctg tccatcagct tgtccagcag ggttgtccac gggccgagcg aagcgagcca
     4861 gccggtggcc gctcgcggcc atcgtccaca tatccacggg ctggcaaggg agcgcagcga
     4921 ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc cgcagccgcc gtaggcggtc
     4981 acgactttgc gaagcaaagt ctagtgagta tactcaagca ttgagtggcc cgccggaggc
     5041 accgccttgc gctgcccccg tcgagccggt tggacaccaa aagggagggg caggcatggc
     5101 ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg ggcaacgtgg cggccagtct
     5161 caagcacgcc taccgcgagc gcgagacgcc caacgctgac gccagcagga cgccagagaa
     5221 cgagcactgg gcggccagca gcaccgatga agcgatgggc cgactgcgcg agttgctgcc
     5281 agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac gtcatgacgg ccagcccgga
     5341 atggtggaag tcggccagcc aagaacagca ggcggcgttc ttcgagaagg cgcacaagtg
     5401 gctggcggac aagtacgggg cggatcgcat cgtgacggcc agcatccacc gtgacgaaac
     5461 cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag gacggcaggc tgtcggccaa
     5521 ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag accacgtttg cggccgctgt
     5581 ggccgatcta gggctgcaac ggggcatcga gggcagcaag gcacgtcaca cgcgcattca
     5641 ggcgttctac gaggccctgg agcggccacc agtgggccac gtcaccatca gcccgcaagc
     5701 ggtcgagcca cgcgcctatg caccgcaggg attggccgaa aagctgggaa tctcaaagcg
     5761 cgttgagacg ccggaagccg tggccgaccg gctgacaaaa gcggttcggc aggggtatga
     5821 gcctgcccta caggccgccg caggagcgcg tgagatgcgc aagaaggccg atcaagccca
     5881 agagacggcc cgag
//
