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pAOX1-αfactor-MCS-6×His-Zeo vector (V017517)

Basic Vector Information

Vector Name:
pAOX1-αfactor-MCS-6×His-Zeo
Antibiotic Resistance:
Zeocin
Length:
3529 bp
Type:
Protein expression
Replication origin:
ori
Host:
Yeast
Selection Marker:
Zeo
Copy Number:
Low
Promoter:
AOX1
5' Primer:
5'AOX
Growth Strain(s):
DH5a
Growth Temperature:
37℃
Expression Method:
Methanol induction

pAOX1-αfactor-MCS-6×His-Zeo vector Map

Copy Sequence View Map
pAOX1-αfactor-MCS-6×His-Zeo3529 bp6001200180024003000AOX1 promoterα-factor secretion signal6xHisAOX1 terminatorTEF1 promoterEM7 promoterBleoRCYC1 terminatorori

pAOX1-αfactor-MCS-6×His-Zeo vector Sequence

LOCUS       .                       3529 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        2..940
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /label="α-factor secretion signal"
                     /note="N-terminal secretion signal from S. cerevisiae
                     alpha-factor"
     CDS             1256..1273
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1353..1599
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        1614..2025
                     /label="TEF1 promoter"
                     /note="promoter for EF-1α"
     promoter        2033..2080
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             2099..2470
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      2539..2786
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2861..3449)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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