LOCUS       .                       3529 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        2..940
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /label="α-factor secretion signal"
                     /note="N-terminal secretion signal from S. cerevisiae
                     alpha-factor"
     CDS             1256..1273
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1353..1599
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        1614..2025
                     /label="TEF1 promoter"
                     /note="promoter for EF-1α"
     promoter        2033..2080
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             2099..2470
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      2539..2786
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2861..3449)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga aaagagaggc
     1201 tgaagctgaa ttcgctagcg tcgaccatat ggggcccgca tgctctagag gtacccatca
     1261 tcatcatcat cattgagttt gtagccttag acatgactgt tcctcagttc aagttgggca
     1321 cttacgagaa gaccggtctt gctagattct aatcaagagg atgtcagaat gccatttgcc
     1381 tgagagatgc aggcttcatt tttgatactt ttttatttgt aacctatata gtataggatt
     1441 ttttttgtca ttttgtttct tctcgtacga gcttgctcct gatcagccta tctcgcagct
     1501 gatgaatatc ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt tttcttggta
     1561 tttcccactc ctcttcagag tacagaagat taagtgagac cttcgtttgt gcggatcccc
     1621 cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac
     1681 tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat tttccctctt
     1741 tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagagacc
     1801 gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc
     1861 ttgaaatttt tttttttagt ttttttctct ttcagtgacc tccattgata tttaagttaa
     1921 taaacggtct tcaatttctc aagtttcagt ttcatttttc ttgttctatt acaacttttt
     1981 ttacttcttg ttcattagaa agaaagcata gcaatctaat ctaaggggcg gtgttgacaa
     2041 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     2101 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     2161 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     2221 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     2281 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     2341 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     2401 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     2461 ggagcaggac tgacacgtcc gacggcggcc cacgggtccc aggcctcgga gatccgtccc
     2521 ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca cgccctcccc
     2581 ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
     2641 atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt
     2701 ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt
     2761 tgggacgctc gaaggcttta atttgcaagc tggagaccaa catgtgagca aaaggccagc
     2821 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2881 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2941 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3001 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     3061 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3121 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3181 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3241 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3301 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3361 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3421 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3481 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatc
//
