Basic Vector Information
- Vector Name:
- pOpen-Exonuclease III E. coli
- Antibiotic Resistance:
- Ampicillin
- Length:
- 2930 bp
- Type:
- Synthetic biology
- Replication origin:
- ori
- Host:
- E. coli
- Growth Temperature:
- 37℃
pOpen-Exonuclease III E. coli vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pOpen-Exonuclease III E. coli vector Sequence
LOCUS V017432 2930 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V017432
VERSION V017432
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 2930)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..2930
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 26..829
/gene="xthA"
/label="Exodeoxyribonuclease III"
/note="Exodeoxyribonuclease III from Escherichia coli
(strain K12). Accession#: P09030"
terminator 964..1011
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(1016..1032)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(1099..1687)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1881..2738)
/label="AmpR"
/note="beta-lactamase"
promoter complement(2739..2843)
/label="AmpR promoter"
primer_bind 2859..2875
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
terminator 2876..2907
/label="tonB terminator"
/note="bidirectional E. coli tonB-P14 transcription
terminator"
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