LOCUS       V017432                 2930 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017432
VERSION     V017432
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..829
                     /gene="xthA"
                     /label="Exodeoxyribonuclease III"
                     /note="Exodeoxyribonuclease III from Escherichia coli
                     (strain K12). Accession#: P09030"
     terminator      964..1011
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     primer_bind     complement(1016..1032)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(1099..1687)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1881..2738)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2739..2843)
                     /label="AmpR promoter"
     primer_bind     2859..2875
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      2876..2907
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
ORIGIN
        1 taagacgacg accctcgggt ctctaatgaa attcgtttct ttcaacatca acgggctgcg
       61 cgctcgtccg caccagcttg aagcgatcgt tgagaaacac cagccggacg ttatcggcct
      121 gcaagaaacc aaagttcatg atgatatgtt tccgctggag gaagtagcca aacttggtta
      181 caacgttttc tatcacggtc agaaaggcca ttacggtgtt gctctgctga ctaaagaaac
      241 cccgatcgcg gttcgtcgtg gcttcccggg cgacgatgag gaagctcagc gccgcatcat
      301 catggcagaa atcccgtccc tgctgggcaa cgttaccgtg attaacggct acttcccgca
      361 aggtgaatct cgtgaccatc cgatcaaatt cccggctaag gcacagttct accagaacct
      421 gcaaaactat ctggaaacag aactcaaacg cgataaccca gttctgatca tgggcgacat
      481 gaacatttcg ccgaccgatc tggacatcgg catcggtgaa gaaaaccgca aacgctggct
      541 gcgcactggt aagtgctctt tcctcccgga agaacgcgaa tggatggatc gtctgatgag
      601 ctggggtctg gtggacacct tccgtcatgc aaacccgcag actgcggatc gtttctcctg
      661 gttcgattac cgttccaaag gtttcgacga taaccgtggc ctgcgcattg acctgctgct
      721 ggcttctcag ccgctggcag aatgctgcgt cgaaaccggc atcgactatg aaattcgttc
      781 tatggagaaa ccttctgatc acgctccggt gtgggcgaca ttccgccgtt gaagagcttt
      841 gagaccgtct catgctagcc acgtatcgcg gtccttgcca gcttctatag ggataacagg
      901 gtaatgtcat atctttgttg acccaccgct gaggtgtcaa tcgtcggagc cgctgagcaa
      961 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gcatggtcat
     1021 agctgtttcc tgagagcttg gcaggtgatg acacacatta acaaatttcg tgaggagtct
     1081 ccagaagaat gccattaatt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1141 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1201 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1261 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1321 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1381 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1441 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1501 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1561 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1621 caccgctggt agcggtggtt tttttgtttg caagcagcag attacacgca gaaaaaaagg
     1681 atctcaagaa ggcctactat tagcaacaac gatcctttga tcttttctac ggggtctgac
     1741 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1801 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1861 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1921 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1981 ggcttaccat ctggccccag tgctgcaatg ataccgcgag aaccacgctc accggctcca
     2041 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2101 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2161 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2221 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2281 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2341 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2401 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2461 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2521 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2581 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2641 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2701 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2761 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2821 aataaacaaa taggggttcc gcgcacctgc accagtcagt aaaacgacgg ccagtagtca
     2881 aaagcctccg accggaggct tttgacttgg ttcaggtgga gtgggagaaa
//
