FUGW vector (V012407)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012407 FUGW In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Plasmid pFUGW was constructed by inserting the following into the multicloning site of HR'CS-G: HIV-1 flap sequence PCR-amplified from the HIV NLA4.3 genome, the human polyubiquitin promoter-C (gift of L. Thiel, Amgen), the EGFP gene, and the WRE (woodchuck hepatitis virus posttranscriptional regulatory element) (gift of D. Trono, University of Geneva). Lentiviruses can be produced by cotransfecting the HIV-1 packaging vector Delta8.9 and the VSVG envelope glycoprotein into 293 fibroblasts.Order of elements: CMV LTR PstI flap PacI Ubiquitin promoter SpeI HindIII PstI SalI XbaI BamHI SmaI KpnI GFP NotI EagI XbaI EcoRI EcoRV HindIII ClaI WRE ClaI SalI XhoI KpnI 3'LTR ApaI PmeI.Please note that there are 2 gaps upstream of the EGFP between author's provided sequence and Addgene's quality control sequence. These gaps are in the non-coding vector region and should not affect the expression of EGFP.

      • Vector Name:
      • FUGW
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 9955 bp
      • Type:
      • Lentiviral vectors
      • Copy Number:
      • High copy number
      • Promoter:
      • hUbc
      • Cloning Method:
      • Enzyme digestion and ligation
      • 5' Primer:
      • 3' Primer:
      • Fusion Tag:
      • C-EGFP
      • Growth Strain(s):
      • Stbl3
      • Growth Temperature:
      • 37℃

FUGW vector Vector Map

FUGW9955 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600CMV enhancerCMV promoter5' LTR (truncated)HIV-1 PsiRREcPPT/CTSUbC promoterKozak sequenceWPREKS primer5' LTR (truncated)bGH poly(A) signalf1 oriSV40 promoterEM7 promoterBleoRSV40 poly(A) signalM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.


  • Dong H, Du L, Cai S, Lin W, Chen C, Still M, Yao Z, Coppes RP, Pan Y, Zhang D, Gao S, Zhang H. Tyrosine Phosphatase PTPRO Deficiency in ERBB2-Positive Breast Cancer Contributes to Poor Prognosis and Lapatinib Resistance. Front Pharmacol. 2022 Apr 1;13:838171.

FUGW vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                9955 bp ds-DNA     circular SYN 05-AUG-2018
DEFINITION  3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for 
            cDNA expresion.
KEYWORDS    fugw
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Lois C, Hong EJ, Pease S, Brown EJ, Baltimore D
  TITLE     Germline transmission and tissue-specific expression of transgenes 
            delivered by lentiviral vectors.
  JOURNAL   Science. 2002 Feb 1. 295(5556):868-72.
  PUBMED    11786607
REFERENCE   2  (bases 1 to 9955)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        238..617
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
     LTR             835..1015
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
     misc_feature    2440..2557
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2622..3833
                     /note="UbC promoter"
                     /note="human ubiquitin C promoter"
     regulatory      3881..3890
                     /note="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3887..4606
                     /product="enhanced GFP"
                     /note="mammalian codon-optimized"
     misc_feature    4647..5235
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5118..5129)
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
     primer_bind     complement(5238..5254)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
     LTR             5760..5940
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5972..6196
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6242..6670
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6684..7013
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      6864..6999
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        7061..7108
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             7127..7501
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="confers resistance to bleomycin, phleomycin, and 
     polyA_signal    7631..7752
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7801..7817)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
     protein_bind    7825..7841
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7849..7879)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7894..7915
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8203..8791)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     CDS             complement(8962..9822)
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(9823..9927)
                     /note="AmpR promoter"