LOCUS fugw. 9955 bp DNA circular SYN 05-AUG-2018 DEFINITION 3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion. ACCESSION . VERSION . KEYWORDS fugw. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9955) AUTHORS Lois C, Hong EJ, Pease S, Brown EJ, Baltimore D TITLE Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. JOURNAL Science. 2002 Feb 1. 295(5556):868-72. PUBMED 11786607 REFERENCE 2 (bases 1 to 9955) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Science. 2002 Feb 1. 295(5556):868-72." FEATURES Location/Qualifiers source 1..9955 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 618..820 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 835..1015 /label=5' LTR (truncated) /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 2291..2332 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2622..3833 /label=UbC promoter /note="human ubiquitin C promoter" CDS 3887..4603 /label=EGFP /note="enhanced GFP" misc_feature 4647..5235 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5238..5254) /label=KS primer /note="KS primer" /note="common sequencing primer, one of multiple similar variants" LTR 5760..5940 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 5972..6196 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6242..6670 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6684..7013 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 7061..7108 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 7127..7498 /label=BleoR /note="antibiotic-binding protein" polyA_signal 7631..7764 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7801..7817) /label=M13 rev /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 7825..7841 /label=lac repressor encoded by lacI binding site /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7849..7879) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7894..7915) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8203..8791) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8965..9822) /label=AmpR /note="beta-lactamase" promoter complement(9823..9927) /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt 2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg 2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga 2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc 2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg 2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc 2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga 3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc 3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt 3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt 3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg 3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc 3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg 3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc 3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt 3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc 3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct 3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc 3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg 3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt 3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt 3841 gggctgcagg tcgactctag aggatccccg ggtaccggtc gccaccatgg tgagcaaggg 3901 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 3961 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 4021 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 4081 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 4141 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 4201 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 4261 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 4321 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 4381 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 4441 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 4501 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 4561 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc 4621 tagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat 4681 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 4741 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 4801 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 4861 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 4921 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 4981 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 5041 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 5101 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 5161 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 5221 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca 5281 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 5341 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 5401 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 5461 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 5521 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca 5581 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct 5641 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga 5701 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg 5761 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 5821 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 5881 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 5941 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 6001 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 6061 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 6121 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 6181 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 6241 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 6301 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 6361 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 6421 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 6481 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 6541 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 6601 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 6661 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 6721 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 6781 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 6841 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 6901 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 6961 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 7021 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc 7081 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac 7141 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga 7201 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga 7261 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg 7321 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa 7381 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga 7441 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg 7501 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 7561 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 7621 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 7681 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 7741 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 7801 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 7861 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 7921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 7981 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 8041 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 8101 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8161 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8221 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8281 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8341 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8401 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 8461 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 8521 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 8581 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 8641 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 8701 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 8761 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 8821 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 8881 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 8941 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 9001 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 9061 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 9121 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 9181 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 9241 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 9301 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 9361 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 9421 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 9481 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 9541 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 9601 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 9661 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 9721 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 9781 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 9841 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 9901 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac //