pMV306hsp vector (V017276)

MSDS

Basic Vector Information

Vector Name:: pMV306hsp

Antibiotic Resistance:: Kanamycin

Length:: 4373 bp

Type:: Gene expression

Replication origin:: ori

Host:: Mycobacterium

Copy Number:: Low

Growth Strain(s):: DH5a

Growth Temperature:: 37℃

pMV306hsp vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMV306hsp vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V017276                 4373 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017276
VERSION     V017276
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             511..1623
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     CDS             1907..2719
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3054..3642
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      4291..4337
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"

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