LOCUS       Exported                4356 bp DNA     circular SYN 13-JAN-2025
DEFINITION  Exported.
ACCESSION   V017276
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4356)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(2451..4356,1..2450)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..813
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1148..1736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2382..2428
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             2961..4073
                     /gene="33"
                     /label=Integrase
                     /note="Integrase from Mycobacterium phage L5. Accession#: 
                     P22884"
ORIGIN
        1 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
       61 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      121 tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      181 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      241 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      301 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      361 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      421 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      481 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      541 gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      601 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      661 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      721 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      781 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
      841 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt
      901 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa
      961 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct
     1021 ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt
     1081 cttcacgagg cagacctcac tagttccact gagcgtcaga ccccgtagaa aagatcaaag
     1141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1261 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1501 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     1561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1741 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1861 ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg ccgctctaga cggtgaccac
     1921 aacgcgcccg ctttgatcgg ggacgtctgc ggccgaccat ttacgggtct tgttgtcgtt
     1981 ggcggtcatg ggccgaacat actcacccgg atcggagggc cgaggacaag gtcgaacgag
     2041 gggcatgacc cggtgcgggg cttcttgcac tcggcatagg cgagtgctaa gaataacgtt
     2101 ggcactcgcg accggtgagt gctaggtcgg gacggtgagg ccaggcccgt cgtcgcagcg
     2161 agtggcagcg aggacaactt gagccgtccg tcgcgggcac tgcgcccggc cagcgtaagt
     2221 agcggggttg ccgtcacccg gtgacccccg tttcatcccc gatccggagg aatcacttcg
     2281 caatggccaa gacaattgcg gatcccccgg gctgcaggaa ttcgatatca agcttatcga
     2341 tgtcgacgta gttaactagc gtacgatcga ctgccaggca tcaaataaaa cgaaaggctc
     2401 agtcgaaaga ctgggccttt cgttttatgc catcatggcc gcggtgatca gctagagccg
     2461 tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct gcaactcccg
     2521 gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg tgtgaatgcc
     2581 cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa agtagccaga
     2641 tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag agttgcagac
     2701 ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga gcgggcgacg
     2761 ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac atatatgggc
     2821 cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa gaaattgcag
     2881 gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg tagccttgtt
     2941 cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag actcagcgca
     3001 gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc
     3061 agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag cggctcatcg
     3121 agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc agcgccatca
     3181 cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac ggcaccaggg
     3241 atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa gtggcggtca
     3301 cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga
     3361 ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca
     3421 agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag cgcgacgtag
     3481 aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag cactaccgga
     3541 tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc gagcttcgcc
     3601 gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc
     3661 gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga
     3721 cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt acgaagatga
     3781 acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg tcgaagtccg
     3841 cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa ctccgcatcc
     3901 acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg accaaggagc
     3961 tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag atggcgtctg
     4021 aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc tcctgaaacg
     4081 caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt gtcagtacgc
     4141 gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga cctgggcacc
     4201 agccccgccg ccgccaggag cattgccgtt cccgccagct agcaacaaag cgacgttgtg
     4261 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     4321 ctgtctgctt acataaacag taatacaagg ggtgtt
//
