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GBX vector (V017209)

Basic Vector Information

Vector Name:
GBX
Antibiotic Resistance:
Ampicillin
Length:
9062 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Promoter:
SFFV
Growth Strain(s):
DH5a
Growth Temperature:
37℃

GBX vector Vector Map

GBX9062 bp40080012001600200024002800320036004000440048005200560060006400680072007600800084008800SFFV promoterEGFPBCL2L1WPRESV40 poly(A) signalAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GBX vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_1031        9062 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat
                     (LTR) promoter"
     CDS             427..1143
                     /label=EGFP
                     /note="enhanced GFP"
     CDS             1210..1908
                     /gene="BCL2L1"
                     /label=BCL2L1
                     /note="Bcl-2-like protein 1 from Homo sapiens. Accession#: 
                     Q07817"
     misc_feature    1928..2505
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    complement(2507..2641)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7156..7260
                     /label=AmpR promoter
     CDS             7261..8118
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8292..8880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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