LOCUS       V017209                 9062 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017209
VERSION     V017209
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..408
                     /label="SFFV promoter"
                     /note="spleen focus-forming virus long terminal repeat
                     (LTR) promoter"
     CDS             427..1143
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1210..1908
                     /gene="BCL2L1"
                     /label="Bcl-2-like protein 1"
                     /note="Bcl-2-like protein 1 from Homo sapiens. Accession#:
                     Q07817"
     misc_feature    1928..2505
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    complement(2507..2641)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        7156..7260
                     /label="AmpR promoter"
     CDS             7261..8118
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8292..8880
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
       61 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
      121 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
      181 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
      241 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
      301 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
      361 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccgggta ccgcgctagc
      421 gccagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      481 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      541 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      601 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      661 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      721 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      781 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      841 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      901 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      961 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1021 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     1081 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1141 aagggatccg gagccacgaa cttctctctg ttaaagcaag caggagacgt ggaagaaaac
     1201 cccggtccta tgtctcagag caaccgggag ctggtggttg actttctctc ctacaagctt
     1261 tcccagaaag gatacagctg gagtcagttt agtgatgtgg aagagaacag gactgaggcc
     1321 ccagaaggga ctgaatcgga gatggagacc cccagtgcca tcaatggcaa cccatcctgg
     1381 cacctggcag acagccccgc ggtgaatgga gccactggcc acagcagcag tttggatgcc
     1441 cgggaggtga tccccatggc agcagtaaag caagcgctga gggaggcagg cgacgagttt
     1501 gaactgcggt accggcgggc attcagtgac ctgacatccc agctccacat caccccaggg
     1561 acagcatatc agagctttga acaggtagtg aatgaactct tccgggatgg ggtaaactgg
     1621 ggtcgcattg tggccttttt ctccttcggc ggggcactgt gcgtggaaag cgtagacaag
     1681 gagatgcagg tattggtgag tcggatcgca gcttggatgg ccacttacct gaatgaccac
     1741 ctagagcctt ggatccagga gaacggcggc tgggatactt ttgtggaact ctatgggaac
     1801 aatgcagcag ccgagagccg aaagggccag gaacgcttca accgctggtt cctgacgggc
     1861 atgactgtgg ccggcgtggt tctgctgggc tcactcttca gtcggaaatg agtttaaacg
     1921 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1981 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2041 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2101 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2161 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2221 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2281 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     2341 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2401 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2461 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggcggatc cagacatgat
     2521 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2581 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2641 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2701 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg
     2761 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     2821 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     2881 gggtgtcggg gcgcagccat gaggtcgact ctagaggatc gatccccgcc ccggacgaac
     2941 taaacctgac tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag
     3001 ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa
     3061 cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa
     3121 cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg
     3181 cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc
     3241 aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct
     3301 accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa
     3361 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
     3421 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
     3481 gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg
     3541 tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag
     3601 gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta
     3661 atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt
     3721 tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg
     3781 acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat
     3841 tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt
     3901 ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg
     3961 ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta
     4021 tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta
     4081 acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg
     4141 ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg
     4201 cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac
     4261 tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag
     4321 taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca
     4381 cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag
     4441 cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg
     4501 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     4561 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
     4621 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     4681 ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg
     4741 ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat
     4801 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag cataggctat
     4861 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4921 cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag catatgatac
     4981 ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa gggggcgtga
     5041 attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa tttaaggagg
     5101 ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta atgttttcca
     5161 acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat gcccaattgc
     5221 cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca ccaacagcac
     5281 gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct tttaatacga
     5341 ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc atctccatca
     5401 cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc caccataggt
     5461 ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac cctgatattg
     5521 caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa aacctcagca
     5581 aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct tagcgggcca
     5641 ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg tgtaagacga
     5701 cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt cttactacct
     5761 ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc
     5821 ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt ggaacctcct
     5881 tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc accctgaccc
     5941 cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc
     6001 gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct
     6061 tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg
     6121 gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc
     6181 cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct
     6241 ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct
     6301 cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct
     6361 cctgctcctg ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt
     6421 ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt
     6481 ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt
     6541 tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca
     6601 tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag
     6661 acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac
     6721 agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc
     6781 cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg
     6841 ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc
     6901 ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc
     6961 accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca
     7021 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
     7081 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7141 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7201 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7261 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7321 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7381 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7441 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7501 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7561 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7621 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7681 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7741 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7801 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7861 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7921 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     7981 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8041 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8101 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8161 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8221 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8281 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8341 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8401 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     8461 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8521 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8581 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8641 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8701 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8761 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8821 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8881 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc
     8941 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     9001 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gagctagctg
     9061 ca
//
