Basic Vector Information
- Vector Name:
- pLenti PGK V5-LUC Neo (w623-2)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9916 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Neo/G418
- Promoter:
- mPGK
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pLenti PGK V5-LUC Neo (w623-2) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLenti PGK V5-LUC Neo (w623-2) vector Sequence
LOCUS 62056_13865 9916 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9916) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..9916 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 232..253 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 268..298 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 306..322 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 330..346 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 367..385 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 413..639 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 640..820 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 867..992 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1485..1718 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1903..1947 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 2096..2137 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 2214..2331 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2377..2887 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" protein_bind 2942..2966 /label=attB1 /note="recombination site for the Gateway(R) BP reaction" CDS 2978..3019 /label=V5 tag /note="epitope tag from simian virus 5" CDS 3095..4744 /label=luciferase /note="firefly luciferase" protein_bind complement(4754..4778) /label=attB2 /note="recombination site for the Gateway(R) BP reaction" misc_feature 4809..5397 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" promoter 5440..5939 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 5953..6753 /label=NeoR/KanR /note="aminoglycoside phosphotransferase from Tn5" LTR 6847..7080 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7152..7286 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 7313..7448 /label=SV40 ori /note="SV40 origin of replication" promoter complement(7469..7487) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7497..7513) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 7655..8110 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 8136..8240 /label=AmpR promoter CDS 8241..9098 /label=AmpR /note="beta-lactamase" rep_origin 9272..9860 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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