LOCUS       V017208                 9916 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017208
VERSION     V017208
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    232..253
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        268..298
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     330..346
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        367..385
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        413..639
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             640..820
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    867..992
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1485..1718
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1903..1947
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2096..2137
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2214..2331
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2377..2887
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     protein_bind    2942..2966
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2978..3019
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             3095..4744
                     /label="luciferase"
                     /note="firefly luciferase"
     protein_bind    complement(4754..4778)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4809..5397
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     promoter        5440..5939
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5953..6753
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     LTR             6847..7080
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7152..7286
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7313..7448
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7469..7487)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7497..7513)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      7655..8110
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8136..8240
                     /label="AmpR promoter"
     CDS             8241..9098
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      9272..9860
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac
     2341 gagactagcc tcgagaagct tgatatcgaa ttccacgggg ttggggttgc gccttttcca
     2401 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
     2461 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
     2521 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
     2581 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
     2641 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
     2701 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
     2761 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
     2821 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     2881 tccccagggg gatccaccgg ttcgtcgact agtccagtgt ggtggaattc tgcagatatc
     2941 aacaagtttg tacaaaaaag caggctccac catggaaggt aagcctatcc ctaaccctct
     3001 cctcggtctc gattctacgg gatccatggg aaccaattca gtccctaggc ttttgcaaaa
     3061 agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa acataaagaa
     3121 aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc
     3181 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
     3241 ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
     3301 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
     3361 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
     3421 taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc
     3481 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat
     3541 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac
     3601 atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga
     3661 caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc
     3721 tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa
     3781 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat
     3841 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga
     3901 agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc
     3961 aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt
     4021 acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa
     4081 gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat
     4141 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt
     4201 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga
     4261 actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa
     4321 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga
     4381 cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt
     4441 ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt
     4501 cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca
     4561 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc
     4621 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa
     4681 actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc
     4741 cgtgtaattc tagacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc
     4801 cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4861 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4921 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4981 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5041 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5101 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5161 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     5221 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5281 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5341 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     5401 gaattctgca gatatccgga ctagtgatct aattctaccg ggtaggggag gcgcttttcc
     5461 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     5521 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     5581 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     5641 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     5701 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     5761 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     5821 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     5881 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     5941 cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
     6001 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6061 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6121 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     6181 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     6241 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     6301 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6361 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6421 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6481 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     6541 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6601 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6661 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6721 atcgccttct atcgccttct tgacgagttc ttctgagggg atccgtcgag ggcccggtac
     6781 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6841 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6901 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6961 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7021 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7081 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7141 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7201 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7261 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     7321 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7381 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7441 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7501 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7561 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7621 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     7681 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7741 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7801 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7861 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7921 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7981 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8041 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     8101 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8161 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8221 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8281 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8341 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8401 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8461 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8521 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8581 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8641 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8701 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8761 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8821 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8881 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8941 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9001 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9061 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9121 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9181 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9241 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9301 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9361 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9421 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9481 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9541 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     9601 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     9661 agctatgaga aagcgccacg cttcccgaag ggagaaaggt ggacaggtat ccggtaagcg
     9721 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9781 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9841 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9901 gctggccttt tgctca
//
