Basic Vector Information
- Vector Name:
- BTV
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9363 bp
- Type:
- Protein expression, Tetracycline inducible
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Promoter:
- CMV
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
BTV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
BTV vector Sequence
LOCUS 62056_436 9363 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9363)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..9363
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(944..1801)
/label=AmpR
/note="beta-lactamase"
promoter complement(1802..1906)
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 2529..2545
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2555..2573
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(2594..2729)
/direction=LEFT
/label=SV40 ori
/note="SV40 origin of replication"
polyA_signal complement(2756..2890)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
LTR complement(2962..3195)
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
misc_feature complement(3282..3870)
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
protein_bind complement(5261..5279)
/label=tet operator
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 5441..6157
/label=EGFP
/note="enhanced GFP"
misc_feature complement(6714..6831)
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(6908..6949)
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
CDS complement(7098..7142)
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
misc_feature complement(7327..7560)
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature complement(8053..8178)
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
promoter complement(8420..8623)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(8624..9003)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter complement(9104..9122)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(9143..9159)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(9167..9183)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9191..9221)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(9236..9257)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
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