LOCUS       V017046                 9363 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017046
VERSION     V017046
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(944..1801)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1802..1906)
                     /label="AmpR promoter"
     rep_origin      complement(1932..2387)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2529..2545
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2555..2573
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2594..2729)
                     /direction=LEFT
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     polyA_signal    complement(2756..2890)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             complement(2962..3195)
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    complement(3282..3870)
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     protein_bind    complement(5261..5279)
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             5441..6157
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    complement(6714..6831)
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             complement(6908..6949)
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     CDS             complement(7098..7142)
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     misc_feature    complement(7327..7560)
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     misc_feature    complement(8053..8178)
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     promoter        complement(8420..8623)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(8624..9003)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(9104..9122)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9143..9159)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(9167..9183)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9191..9221)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9236..9257)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     2521 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
     2581 gggtacgtcc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     2641 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     2701 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc
     2761 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2821 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2881 taaacaagtt cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa
     2941 taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     3001 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     3061 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     3121 tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt
     3181 gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct
     3241 tgtaagtcat tggtcttaaa ggtaccgagc tcgaattcca ggcggggagg cggcccaaag
     3301 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
     3361 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     3421 cccgcgcaga atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc
     3481 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
     3541 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
     3601 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
     3661 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
     3721 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
     3781 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
     3841 ttcacaaatt ttgtaatcca gaggttgatt gtcgactcta gactcgactc tagaggatcc
     3901 ccctgttcca cctcttgaag gctatgtagg ccacaaggcc cacaaccaca gcagccagga
     3961 tggagcaata gacagggatg aggttgtcgg tggtgcctcg ggtcaccacg ggctgggagc
     4021 tgcccatcac tgtggtcacc acacctgcca ccgtgctggc tatgaggtct tgttctggag
     4081 gtgcctcagg ctcctgggtg ctgggggctg tgctgtccga gccctctggg ggtgtggacc
     4141 gtgtaatcca acggccaggg atctcctcgc actcggcgtc ggcccagcgt gtgcactcgc
     4201 ggagctggcg ctcggtgtcc tcgcacacgg tgcagggcag gcacgggtcc acgtggttgg
     4261 cctcgtcgga atacgtgccg tcggggcact cctcgcacac ggtgttctgc ttgtcctggc
     4321 aggagaacac gaggcccgag cccgcctcgc acacgcggca cgcctcgcag cgcccagtcg
     4381 tctcatcctg gtagtagccg taggcgcagc ggcacacggc gtcgtcggcc tccacgcacg
     4441 gcgccgacat gctctggagc cccacgcact cggtgcacgg cttgcacggc tcggtcgcgc
     4501 tcaccacgtc ggagaacgtc acgctgtcca ggcagggctc acacacggtc tggttggctc
     4561 cacaaggctg ggccacaccc tcgcccaggt tgcaggcttt gcagcactca ccgctgtgtg
     4621 tgtacaggcc tgtggggcat gcctccttgg cacctccaag ggacaccccc agaagcagca
     4681 acagcagcag gcgcggcccg tccatggcgc ggccggtggc acctgccccc atcgcccgcc
     4741 tcccgcggca gcgctcgact tccagctcgg tccgctttgc ggactgatgg ggctgcgctg
     4801 cgctgcgctc cagcgccccc cctgcccgcc ggagctggcc gcggcccgaa ttccctagag
     4861 gatccccggg taccgagctc gaattcgggg ccgcggaggc tggatcggtc ccggtgtctt
     4921 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
     4981 gctctgctta tataggcctc ccaccgtaca cgcctacctc gacccgggta ccgagctcga
     5041 ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc
     5101 actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa
     5161 actcgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
     5221 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     5281 tggtaaactc gacctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     5341 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
     5401 ccgaattcct gcagcggccc tagcgctacc ggtcgccacc atggtgagca agggcgagga
     5461 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5521 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     5581 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5641 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5701 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5761 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5821 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5881 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5941 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     6001 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     6061 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     6121 cgccgggatc actctcggca tggacgagct gtacaagtag cggccggccg cgtcgaccta
     6181 gttctagcta gcaactacgc gtagtcgata tccttacctg cagggccttt aattaaactg
     6241 gctagcttag tcatgcaccg gtggatccag accacctccc ctgcgagcta agctggacag
     6301 ccaatgacgg gtaagagagt gacatttttc actaacctaa gacaggaggg ccgtcagagc
     6361 tactgcctaa tccaaagacg ggtaaaagtg ataaaaatgt atcactccaa cctaagacag
     6421 gcgcagcttc cgagggattt gagatccaga catgataaga tacattgatg agtttggaca
     6481 aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc
     6541 cttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     6601 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     6661 atgtggtatg gctgattatg atcagatctc tcgaggctag tctcgtgatc gataaaattt
     6721 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
     6781 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa agttaaccga
     6841 taccgtcgag atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct
     6901 tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat
     6961 aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt
     7021 aaacctacca agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt
     7081 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
     7141 ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta
     7201 atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta
     7261 ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa
     7321 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc
     7381 tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc
     7441 aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc
     7501 attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct
     7561 attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact
     7621 cctaatggtt caatttttac tactttatat ttatataatt cacttctcca attgtccctc
     7681 atatctcctc ctccaggtct gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt
     7741 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     7801 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     7861 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     7921 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     7981 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     8041 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     8101 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     8161 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     8221 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     8281 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     8341 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     8401 gaccccggtt cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc
     8461 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
     8521 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
     8581 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
     8641 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     8701 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     8761 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     8821 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     8881 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
     8941 atgggctatg aactaatgac cccgtaattg attactatta ataactagtc aataatcaat
     9001 gtcaacatgg cggtaatgtt ggacatgagc caatataaat gtacatatta tgatatggat
     9061 acaacgtatg caatggccaa gcttgcagct ccagcttttg ttccctttag tgagggttaa
     9121 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     9181 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     9241 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     9301 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     9361 gct
//
