Basic Vector Information
- Vector Name:
- GFP-BAZ1A
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10523 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
GFP-BAZ1A vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
GFP-BAZ1A vector Sequence
LOCUS 62056_1046 10523 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10523) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..10523 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 105..484 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 485..688 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 813..1529 /codon_start=1 /label=EmGFP /note="Emerald GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" protein_bind 1545..1569 /label=attB1 /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6220..6244) /label=attB2 /note="recombination site for the Gateway(R) BP reaction" polyA_signal 6377..6425 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 6627..7053 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 7067..7396 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 7444..7491 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 7510..7905 /codon_start=1 /label=BSD /note="blasticidin S deaminase" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 8066..8199 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(8284..8314) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(8329..8350) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8638..9226) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9400..10257) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10258..10362) /label=AmpR promoter
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