LOCUS       62056_1046       10523 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10523)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             813..1529
                     /codon_start=1
                     /label=EmGFP
                     /note="Emerald GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    1545..1569
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6220..6244)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6377..6425
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6627..7053
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7067..7396
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        7444..7491
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             7510..7905
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8066..8199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(8284..8314)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8329..8350)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8638..9226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9400..10257)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10258..10362)
                     /label=AmpR promoter
ORIGIN
        1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
       61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      721 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      781 agaggatccc taccggtgat atcctcgaga ccatggtgag caagggcgag gagctgttca
      841 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      901 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      961 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc
     1021 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1081 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1141 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1201 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1261 aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc
     1321 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1381 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1441 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1501 tcactctcgg catggacgag ctgtacaagg gctcgagccc atcaacaagt ttgtacaaaa
     1561 aagcaggctt cgaaggagat agaaccaatt ctctaaggaa atacttaacc atggtcgact
     1621 ggatccggat gccgctgcta caccgaaagc cgtttgtgag acagaagccg cccgcggacc
     1681 tgcggcccga cgaggaagtt ttctactgta aagtcaccaa cgagatcttc cgccactacg
     1741 atgacttttt tgaacgaacc attctgtgca acagccttgt gtggagttgt gctgtgacgg
     1801 gtagacctgg actgacgtat caggaagcac ttgagtcaga aaaaaaagca agacagaatc
     1861 ttcagagttt tccagaacca ctaattattc cagttttata cttgaccagc cttacccatc
     1921 gttcgcgctt acatgaaatt tgtgatgata tctttgcata tgtcaaggat cgatattttg
     1981 tcgaagaaac tgtggaagtc attaggaaca atggtgcaag gttgcagtgt aggattttgg
     2041 aagtcctccc tccatcacat caaaatggtt ttgctaatgg acatgttaac agtgtggatg
     2101 gagaaactat tatcatcagt gatagtgatg attcagaaac acaaagctgt tcttttcaaa
     2161 atgggaagaa aaaagatgca attgatccct tactattcaa gtataaagtg caacccacta
     2221 aaaaagaatt acatgagtct gctattgtta aagcaacaca aatcagccgg agaaaacacc
     2281 tattttctcg tgataaacta aagctttttc tgaagcaaca ctgtgaacca caagatggag
     2341 tcattaaaat aaaggcatca tctctttcaa cgtataaaat agcagaacaa gatttttctt
     2401 atttcttccc tgatgatcca cccacattta tcttcagtcc tgctaacaga cgaagaggga
     2461 gacctcccaa acgaatacat attagtcaag aggacaatgt tgctaataaa cagactcttg
     2521 caagttatag gagcaaagct actaaagaaa gagataaact tttgaaacaa gaagaaatga
     2581 agtcactggc ttttgaaaag gctaaattaa aaagagaaaa agcagatgcc ctagaagcga
     2641 agaaaaaaga aaaagaagat aaagagaaaa agagggaaga attgaaaaaa attgttgaag
     2701 aagagagact aaagaaaaaa gaagaaaaag agaggcttaa agtagaaaga gaaaaggaaa
     2761 gagagaagtt acgtgaagaa aagcgaaagt atgtggaata cttaaaacag tggagtaaac
     2821 ctagagaaga tatggaatgt gatgacctta aggaacttcc agaaccaaca ccagtgaaaa
     2881 ctagactacc tcctgaaatc tttggtgatg ctctgatggt tttggagttc cttaatgcat
     2941 ttggggaact ttttgatctt caagatgagt ttcctgatgg agtaacccta gaagtattag
     3001 aggaagctct tgtaggaaat gacagtgaag gcccactgtg tgaattgctt tttttcttcc
     3061 tgactgcaat cttccaggca atagctgaag aagaagagga agtagccaaa gagcaactaa
     3121 ctgatgctga caccaaaggc tgcagtttga aaagtttgga tcttgatagc tgcactcttt
     3181 cagaaatcct cagactgcac atcttagctt caggtgctga tgtaacatca gcaaatgcaa
     3241 agtatagata tcaaaaacga ggaggatttg atgctacaga tgatgcttgt atggagcttc
     3301 gtttgagcaa tcccagtcta gtgaagaaac tgtcaagcac ctcagtgtat gatttgacac
     3361 caggagaaaa aatgaagata ctccatgctc tctgtggaaa gctactgacc ctagtttcaa
     3421 ctagggattt tattgaagat tatgttgata tattacgaca ggcaaagcag gagttccggg
     3481 aattaaaagc agaacaacat cgaaaagaga gggaagaagc agctgccaga attcgtaaaa
     3541 ggaaggaaga aaaacttaag gagcaagaac aaaaaatgaa agagaaacaa gaaaaactga
     3601 aagaagatga gcaaagaaat tcaacggcag atatatctat tggggaggaa gaaagggaag
     3661 attttgatac tagcattgag agcaaagaca cagagcaaaa ggaattagat caagatatgg
     3721 tcactgaaga tgaagatgac ccaggatcac ataaaagagg cagaaggggg aaaagaggac
     3781 aaaatggatt taaagaattt acaaggcaag aacagatcaa ctgtgtaaca agagagcctc
     3841 ttactgctga tgaggaagaa gcattaaaac aggaacacca acgaaaagag aaagagctct
     3901 tagaaaaaat ccaaagtgcc atagcctgta ccaatatctt tcccttgggt cgcgaccgca
     3961 tgtatagacg atactggatt ttcccttcta ttcctggact ctttattgaa gaggattatt
     4021 ctggtcttac tgaagacatg ctgttgccta gaccttcatc atttcagaat aatgtacagt
     4081 ctcaagatcc tcaggtatcc actaaaactg gagagccttt gatgtctgaa tctacctcca
     4141 acattgacca aggtccacgt gaccattctg tgcagctgcc aaaaccagtg cataagccaa
     4201 atcggtggtg cttttacagt tcttgtgaac agctagacca gcttattgaa gctcttaatt
     4261 ctagaggaca tagagaaagt gccttaaaag aaactttgtt acaagagaaa agcagaatat
     4321 gtgcacagct agcccgtttt tctgaagaga aatttcattt ttcagacaaa cctcagcctg
     4381 atagcaaacc aacatatagt cggggaagat cttccaatgc atatgatcca tctcagatgt
     4441 gtgcagaaaa gcaacttgaa ctaaggctga gagattttct tttagatatt gaagatagaa
     4501 tctaccaagg aacattagga gccatcaagg ttacagatcg acatatctgg agatcagcat
     4561 tagaaagtgg acggtatgag ctgttaagtg aggaaaacaa ggaaaatggg ataattaaaa
     4621 ctgtgaatga agacgtagaa gagatggaaa ttgatgaaca aacaaaggtc atagtaaaag
     4681 acagactttt ggggataaaa acagaaactc caagtactgt atcaacaaat gcaagtacac
     4741 cacaatcagt gagcagtgtg gttcattatc tggcaatggc actctttcaa atagagcagg
     4801 gcattgagcg gcgttttctg aaagctccac ttgatgccag tgacagtggg cgttcttata
     4861 aaacagttct ggaccgttgg agagagtctc tcctttcttc tgctagtcta tcccaagttt
     4921 ttcttcacct atccaccttg gatcgtagcg tgatatggtc taaatctata ctgaatgcgc
     4981 gttgcaagat atgtcgaaag aaaggcgatg ctgaaaacat ggttctttgt gatggctgtg
     5041 ataggggtca tcatacctac tgtgttcgac caaagctcaa gactgtgcct gaaggagact
     5101 ggttttgtcc agaatgtcga ccaaagcaac gttctagaag actctcctct agacagagac
     5161 catccttgga aagtgatgaa gatgtggaag acagtatggg aggtgaggat gatgaagttg
     5221 atggcgatga agaagaaggt caaagtgagg aggaagagta tgaggtagaa caagatgaag
     5281 atgactctca agaagaggaa gaagtcagcc tacccaaacg aggaagacca caagttagat
     5341 tgccagttaa aacaagaggg aaacttagct cttctttctc aagtcgtggc caacaacaag
     5401 aacctggaag atacccttca aggagtcagc agagcacacc caaaacaact gtttcttcta
     5461 aaactggtag aagcctaaga aagataaact ctgctcctcc tacagaaaca aaatctttaa
     5521 gaattgccag tcgttctact cgccacagtc atggcccact gcaagcagat gtatttgtgg
     5581 aattgcttag tcctcgtaga aaacgcagag gcaggaaaag tgctaataat acaccagaaa
     5641 atagtcccaa cttccctaac ttcagagtca ttgccacaaa gtcaagtgaa cagtcaagat
     5701 ctgtaaatat tgcttcaaaa ctttctctcc aagagagtga atccaaaaga agatgcagaa
     5761 aaagacaatc tccagagcca tcgcctgtga cactgggtcg aaggagttct ggccgacagg
     5821 gaggagttca tgaattgtct gcttttgaac aacttgttgt agaattggta cgacatgatg
     5881 acagctggcc ttttttgaaa cttgtttcta aaatccaggt cccagactac tatgacatca
     5941 tcaaaaagcc cattgcctta aatataattc gtgaaaaagt gaataagtgt gaatataaat
     6001 tagcatctga gtttattgat gacattgagt taatgttttc gaactgcttt gaatacaacc
     6061 ctcgtaacac aagtgaagca aaagctggaa ctaggcttca agcatttttt catattcagg
     6121 ctcaaaagct tggactccac gtcacaccca gtaatgtgga ccaagttagc acaccaccgg
     6181 ctgcgaaaaa gtcacgaatc ttacactcga gatatctaga cccagctttc ttgtacaaag
     6241 tggttgataa cgggaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     6301 gttagtaatg agtttaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa
     6361 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     6421 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     6481 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     6541 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg
     6601 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6661 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6721 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt cccgtcaagc tctaaatcgg
     6781 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaacttgatt
     6841 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6901 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6961 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7021 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     7081 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7141 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7201 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7261 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7321 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7381 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     7441 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     7501 aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     7561 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     7621 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     7681 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     7741 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     7801 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     7861 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc acttcgtggc
     7921 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     7981 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8041 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8101 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8161 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8221 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8281 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8341 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8401 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8461 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8521 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8581 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8641 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8701 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8761 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8821 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8881 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8941 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9001 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9061 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9121 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9181 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9241 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9301 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9361 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9421 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9481 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9541 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9601 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9661 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9721 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9781 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9841 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9901 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9961 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10021 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10081 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10141 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10201 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10261 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10321 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10381 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
    10441 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
    10501 gctgagtagt gcgcgagcaa aat
//
