pP10-rpsLneo vector (V016546)

LOCUS       V016546                 4481 bp    RNA     circular SYN 15-JAN-2020
DEFINITION  Exported.
ACCESSION   V016546
VERSION     V016546
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Liu X, Yang X, Mehboob A, Hu X, Yi Y, Li Y, Zhang Z.
  TITLE     A construction strategy for a baculovirus-silkworm multigene
            expression system and its application for coexpression of type I and
            type II interferons
  JOURNAL   Microbiologyopen, e979 (2020) In press
   PUBMED   31854114
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2019) Biotechnology Research Institute, Chinese
            Academy of Agricultural Sciences, No. 12 Zhongguancun South Street,
            Beijing 100081, China
REFERENCE   3  (bases 1 to 4481)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiologyopen, e979 (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-NOV-2019) Biotechnology Research Institute, Chinese Academy of
            Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing
            100081, China"
FEATURES             Location/Qualifiers
     source          1..4481
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    451..682
                     /gene="rpsL-neo"
                     /label="BmNPV p10 gene recombination sequence"
                     /note="BmNPV p10 gene recombination sequence"
     misc_feature    683..738
                     /gene="rpsL-neo"
                     /label="MCS"
                     /note="MCS"
     CDS             877..1248
                     /gene="rpsL"
                     /label="Small ribosomal subunit protein uS12"
                     /note="Small ribosomal subunit protein uS12 from Salmonella
                     paratyphi A (strain ATCC 9150 / SARB42). Accession#:
                     Q5PIW1"
     CDS             1263..2054
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    2078..2195
                     /gene="rpsL-neo"
                     /label="BmNPV p10 gene recombination sequence"
                     /note="BmNPV p10 gene recombination sequence"
     primer_bind     complement(2260..2276)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2284..2300)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2308..2338)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2353..2374)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2662..3250)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3424..4281)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4282..4386)
                     /label="AmpR promoter"