Basic Vector Information
- Vector Name:
- pP10-rpsLneo
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4481 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Liu X, Yang X, Mehboob A, Hu X
pP10-rpsLneo vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pP10-rpsLneo vector Sequence
LOCUS 62056_18335 4481 bp RNA circular SYN 15-JAN-2020 DEFINITION Cloning vector pP10-rpsLneo, complete sequence. ACCESSION MN702626 VERSION . KEYWORDS . SOURCE ORGANISM REFERENCE 1 (bases 1 to 4481) AUTHORS Liu X, Yang X, Mehboob A, Hu X, Yi Y, Li Y, Zhang Z. TITLE A construction strategy for a baculovirus-silkworm multigene expression system and its application for coexpression of type I and type II interferons JOURNAL Microbiologyopen, e979 (2020) In press PUBMED 31854114 REFERENCE 2 (bases 1 to 4481) AUTHORS Liu X. TITLE Direct Submission JOURNAL Submitted (16-NOV-2019) Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, China REFERENCE 3 (bases 1 to 4481) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Microbiologyopen, e979 (2020) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (16-NOV-2019) Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 100081, China" FEATURES Location/Qualifiers source 1..4481 /mol_type="other DNA" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 451..682 /gene="rpsL-neo" /label=BmNPV p10 gene recombination sequence /note="BmNPV p10 gene recombination sequence" misc_feature 683..738 /gene="rpsL-neo" /label=MCS /note="MCS" CDS 877..1248 /gene="rpsL" /label=rpsL /note="Small ribosomal subunit protein uS12 from Salmonella paratyphi A (strain ATCC 9150 / SARB42). Accession#: Q5PIW1" CDS 1263..2054 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" misc_feature 2078..2195 /gene="rpsL-neo" /label=BmNPV p10 gene recombination sequence /note="BmNPV p10 gene recombination sequence" primer_bind complement(2260..2276) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2284..2300) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2308..2338) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(2353..2374) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2662..3250) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3424..4281) /label=AmpR /note="beta-lactamase" promoter complement(4282..4386) /label=AmpR promoter
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