LOCUS       V016546                 4481 bp    RNA     circular SYN 15-JAN-2020
DEFINITION  Exported.
ACCESSION   V016546
VERSION     V016546
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Liu X, Yang X, Mehboob A, Hu X, Yi Y, Li Y, Zhang Z.
  TITLE     A construction strategy for a baculovirus-silkworm multigene
            expression system and its application for coexpression of type I and
            type II interferons
  JOURNAL   Microbiologyopen, e979 (2020) In press
   PUBMED   31854114
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2019) Biotechnology Research Institute, Chinese
            Academy of Agricultural Sciences, No. 12 Zhongguancun South Street,
            Beijing 100081, China
REFERENCE   3  (bases 1 to 4481)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiologyopen, e979 (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-NOV-2019) Biotechnology Research Institute, Chinese Academy of
            Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing
            100081, China"
FEATURES             Location/Qualifiers
     source          1..4481
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    451..682
                     /gene="rpsL-neo"
                     /label="BmNPV p10 gene recombination sequence"
                     /note="BmNPV p10 gene recombination sequence"
     misc_feature    683..738
                     /gene="rpsL-neo"
                     /label="MCS"
                     /note="MCS"
     CDS             877..1248
                     /gene="rpsL"
                     /label="Small ribosomal subunit protein uS12"
                     /note="Small ribosomal subunit protein uS12 from Salmonella
                     paratyphi A (strain ATCC 9150 / SARB42). Accession#:
                     Q5PIW1"
     CDS             1263..2054
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    2078..2195
                     /gene="rpsL-neo"
                     /label="BmNPV p10 gene recombination sequence"
                     /note="BmNPV p10 gene recombination sequence"
     primer_bind     complement(2260..2276)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2284..2300)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2308..2338)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2353..2374)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2662..3250)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3424..4281)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4282..4386)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa agaagcatga cggcaagtgg
      421 acgattgata tcccaatgca ttggaagctt tggagtcttg tgtgctattt tacagagatt
      481 cagaaatacg catcacttac aacagggggg actatgaaat tatgcatttg aggatgccgg
      541 gacctttaat tcaacccaac acaatatatt acagctaaat aagaattatt attaaattat
      601 ttgtatatta attaaaatct tatactgtaa attacatttt atttactatc atttcaaagc
      661 ctaacgtttt gacacgaatt ttggatccct gcaggcctcg agttcgaatc tagaagatct
      721 ggtaccgagc tcgaattcgg cctggtgatg atggcgggat cgttgtatat ttcttgacac
      781 cttttcggca tcgccctaaa attcggcgtc ctcatattgt gtgaggacgt tttattacgt
      841 gtttacgaag caaaagctaa aaccaggagc tatttaatgg caacagttaa ccagctggta
      901 cgcaaaccac gtgctcgcaa agttgcgaaa agcaacgtgc ctgcgctgga agcatgcccg
      961 caaaaacgtg gcgtatgtac tcgtgtatat actaccactc ctaaaaaacc gaactccgcg
     1021 ctgcgtaaag tatgccgtgt tcgtctgact aacggtttcg aagtgacttc ctacatcggt
     1081 ggtgaaggtc acaacctgca ggagcactcc gtgatcctga tccgtggcgg tcgtgttaaa
     1141 gacctcccgg gtgttcgtta ccacaccgta cgtggtgcgc ttgactgctc cggcgttaaa
     1201 gaccgtaagc aggctcgttc caagtatggc gtgaagcgtc ctaaggctta aggaggacaa
     1261 tcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     1321 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     1381 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     1441 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     1501 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     1561 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     1621 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     1681 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     1741 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     1801 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     1861 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     1921 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     1981 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     2041 ttgacgagtt cttctgagcg gccgcttaat taattgagac ggtttgcccg ctcaattgac
     2101 tgattttaac actaaaatct cagaaattca atccatattg accggtgaca ctgctccgga
     2161 ccctccagaa tcctagagcc taacctgaaa agccagtcga cactagtatc gataatctcc
     2221 agaggatcgc cgggaaccga ggacgagttc gtaatcatgg tcatagctgt ttcctgtgtg
     2281 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     2341 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2401 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2461 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2521 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2581 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2641 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2701 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2761 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2821 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2881 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2941 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3001 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3061 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3121 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3181 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3241 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3301 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3361 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3421 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3481 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3541 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3601 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3661 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3721 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3781 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3841 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3901 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3961 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4021 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4081 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4141 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4201 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4261 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4321 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4381 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     4441 attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
