pMEK18 vector (V016488)

MSDS

Basic Vector Information

Vector Name:: pMEK18

Antibiotic Resistance:: Ampicillin

Length:: 4550 bp

Type:: Transformation vector

Replication origin:: ori

Source/Author:: Yu Q, LaManna L, Kelly ME, Lutz KA

pMEK18 vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMEK18 vector Sequence

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Download GeneBank File(.gb)

LOCUS       62056_16730        4550 bp DNA     circular SYN 08-SEP-2019
DEFINITION  Transformation vector pMEK18, complete sequence.
ACCESSION   MN326017
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Yu Q, LaManna L, Kelly ME, Lutz KA, Maliga P.
  TITLE     New Tools for Engineering the Arabidopsis Plastid Genome
  JOURNAL   Plant Physiol. (2019) In press
  PUBMED    31427463
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Maliga P, Kelly ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2019) Waksman Institute of Microbiology, Rutgers 
            University, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA
REFERENCE   3  (bases 1 to 4550)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-AUG-2019) Waksman Institute of Microbiology, Rutgers University,
            190 Frelinghuysen Road, Piscataway, NJ 08854, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1055
                     /note="left plastid targeting region of vector, A. thaliana
                     RLD plastid DNA"
     misc_feature    complement(1..596)
                     /label=16S ribosomal RNA fragment
                     /note="16S ribosomal RNA fragment"
     tRNA            complement(828..899)
                     /product="tRNA-Val"
     misc_feature    1066..1122
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    1130..1979
                     /note="right plastid targeting region of vector, A.
                     thaliana RLD intergenic plastid DNA"
     promoter        complement(1989..2007)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2014..2030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2172..2627
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2653..2757
                     /label=AmpR promoter
     CDS             2758..3615
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3789..4377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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