pMEK18 vector (V016488)

LOCUS       62056_16730        4550 bp DNA     circular SYN 08-SEP-2019
DEFINITION  Transformation vector pMEK18, complete sequence.
ACCESSION   MN326017
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Yu Q, LaManna L, Kelly ME, Lutz KA, Maliga P.
  TITLE     New Tools for Engineering the Arabidopsis Plastid Genome
  JOURNAL   Plant Physiol. (2019) In press
  PUBMED    31427463
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Maliga P, Kelly ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2019) Waksman Institute of Microbiology, Rutgers 
            University, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA
REFERENCE   3  (bases 1 to 4550)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-AUG-2019) Waksman Institute of Microbiology, Rutgers University,
            190 Frelinghuysen Road, Piscataway, NJ 08854, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1055
                     /note="left plastid targeting region of vector, A. thaliana
                     RLD plastid DNA"
     misc_feature    complement(1..596)
                     /label=16S ribosomal RNA fragment
                     /note="16S ribosomal RNA fragment"
     tRNA            complement(828..899)
                     /product="tRNA-Val"
     misc_feature    1066..1122
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    1130..1979
                     /note="right plastid targeting region of vector, A.
                     thaliana RLD intergenic plastid DNA"
     promoter        complement(1989..2007)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2014..2030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2172..2627
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2653..2757
                     /label=AmpR promoter
     CDS             2758..3615
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3789..4377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"