LOCUS       62056_16730        4550 bp DNA     circular SYN 08-SEP-2019
DEFINITION  Transformation vector pMEK18, complete sequence.
ACCESSION   MN326017
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Yu Q, LaManna L, Kelly ME, Lutz KA, Maliga P.
  TITLE     New Tools for Engineering the Arabidopsis Plastid Genome
  JOURNAL   Plant Physiol. (2019) In press
  PUBMED    31427463
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Maliga P, Kelly ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2019) Waksman Institute of Microbiology, Rutgers 
            University, 190 Frelinghuysen Road, Piscataway, NJ 08854, USA
REFERENCE   3  (bases 1 to 4550)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-AUG-2019) Waksman Institute of Microbiology, Rutgers University,
            190 Frelinghuysen Road, Piscataway, NJ 08854, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1055
                     /note="left plastid targeting region of vector, A. thaliana
                     RLD plastid DNA"
     misc_feature    complement(1..596)
                     /label=16S ribosomal RNA fragment
                     /note="16S ribosomal RNA fragment"
     tRNA            complement(828..899)
                     /product="tRNA-Val"
     misc_feature    1066..1122
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    1130..1979
                     /note="right plastid targeting region of vector, A.
                     thaliana RLD intergenic plastid DNA"
     promoter        complement(1989..2007)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2014..2030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2172..2627
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2653..2757
                     /label=AmpR promoter
     CDS             2758..3615
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3789..4377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tggtagtttc caccgcctgt ccagggttga gccctgggat ttgacggcgg acttaaaaag
       61 ccacctacag acgctttacg cccaatcatt ccggataacg cttgcatcct ctgtattacc
      121 gcggctgctg gcacagagtt agccgatgct tattccccag ataccgtcat tgcttcttct
      181 ctgggaaaag aagttcagga cccgtaggcc ttctacctcc acgcggcatt gctccgtcag
      241 gctttcgccc attgcggaaa attccccact gctgcctccc gtaggagtct gggccgtgtc
      301 tcagtcccag tgtggctgat catcctctcg gaccagctac tgatcatcgc cttggtaagc
      361 tattgcctca ccaactagct aatcagacgc gagcccctcc tcgggcggat tcctcctttt
      421 gctcctcagc ctacggggta ttagcagccg tttccagctg ttgttcccct cccaagggca
      481 ggttcttacg cgttactcac ccgtccgcca ctggaaacac cacttcccgt ccgacttgca
      541 tgtgttaagc atgccgccag cgttcatcct gagccaggat cgaactctcc atgagattca
      601 tagttgcatt acttatagct tccttcttcg tagacaaagc tgattcggaa ttgtctttca
      661 ttccaagtca taacttgtat ccatgcgctt catattcgca tggagttcgc tcccagaaat
      721 atagctaccc ctaccccctc acgtcaatcc cacgagcctc ttatccattc ttattcgatc
      781 acagcgaggg agcaagtcaa aatagaaaaa ctcacattca ttgggtttag ggataatcag
      841 gctcgaactg atgacttcca ccacgtcaag gtgacactct accgctgagt tatatccctt
      901 cccccatcaa gaaatagaac tgactaatcc taagtcaaag ggtcgagaaa ctcaaggcca
      961 ctattcttga acaacttgga ttggagccgg gctttccttt cgcactttat acgggtatga
     1021 aatgaaaata atggaaaaag ttggattcaa ttgtcctatt taaatgaatt cgagctcggt
     1081 acccggggat cctctagagt cgacctgcag gcatgcaagc ttgatatcga ctactcctat
     1141 cggaaatagg attgactacg gattcgagcc atagcacatg gtttcataaa accgtacgat
     1201 tctcccgatc taaatcaagc cggttttaca tgaagaagat ttgactcggc atgttctatt
     1261 cgatacgggt aggagaaacg gtattctttt cttaaacttc aaaaaataga gaaataagaa
     1321 ccaagtcaag atgatacgga ttaatccttt attcttgcgc caaagatctt cctatttcca
     1381 aaggaactgg agttacatct cttttccatt tccattcaag agttcttatg tgtttccacg
     1441 cccctttaag accccgaaaa atcgacaaat tcccttttct taggaccaca tgcgagataa
     1501 cgaaaaaaaa aaagagagaa tggtaacccc acgattaact attttattta tgaatttcat
     1561 agtaatagaa atacatgtcc taccgaaaca gaatttgtaa cttgctatcc tatcatcttg
     1621 cctagcaggc aaagatttca ctccgcgaaa aagatgattc attcggatca acatgaaagc
     1681 ccaactacat tgccagaatt tatatattgg aaagaggttt acctccttgc ttctatggta
     1741 caatcctctt cccgcggagc ctcctttctt ctcggtccgc agagacaaaa tgtaggactg
     1801 gtgccaacag ttaatcacgg aagaaaggac tcactgcgcc aagatcacta actaatctaa
     1861 tagaatagaa aatcctaata taatagaaaa gaaaagaact gtcttttctg tatacttatg
     1921 tatactttcc ccggttccgt tgctactgcg ggctttacgc aattgatcgg atcatctagc
     1981 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     2041 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     2101 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     2161 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2221 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2281 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2341 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2401 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2461 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2521 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2581 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     2641 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2701 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2761 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2821 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2881 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2941 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3001 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3061 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3301 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3781 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3841 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3901 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3961 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4021 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4081 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4141 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4201 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4261 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4321 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4381 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4441 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4501 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
//
