whiteCC vector (V016480)

MSDS

Basic Vector Information

Vector Name:: whiteCC

Antibiotic Resistance:: Ampicillin

Length:: 7130 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Li Z.

Promoter:: dU6-3

whiteCC vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

whiteCC vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_23495        7130 bp DNA     circular SYN 21-APR-2021
DEFINITION  Cloning vector whiteCC, complete sequence.
ACCESSION   MW770350
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Li Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2021) Cell and Developmental Biology, University 
            of California, San Diego, 9500 Gilman Drive Tata Hall 5401, San 
            Diego, CA 92122, USA
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2021) Cell and Developmental Biology, University of 
            California, San Diego, 9500 Gilman Drive Tata Hall 5401, San Diego, 
            CA 92122, USA"
FEATURES             Location/Qualifiers
     source          1..7130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(127..715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(889..1746)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1747..1851)
                     /label=AmpR promoter
     CDS             3215..3889
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     CDS             3899..3919
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4060..4181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4184..4604
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3
                     snRNA (Port et al., 2014)"
     ncRNA           4604..4622
                     /label=sgRNA
                     /note="white intron gRNA"
                     /ncRNA_class="sgRNA"
     misc_RNA        4624..4699
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      4706..4933
                     /label=U6 terminal
                     /note="U6 terminal"
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    complement(4999..5128)
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     CDS             complement(5146..5862)
                     /label=mCerulean
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
     regulatory      6040..6083
                     /label=3XP3
                     /note="3XP3"
                     /regulatory_class="promoter"

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