LOCUS       62056_23495        7130 bp DNA     circular SYN 21-APR-2021
DEFINITION  Cloning vector whiteCC, complete sequence.
ACCESSION   MW770350
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Li Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2021) Cell and Developmental Biology, University 
            of California, San Diego, 9500 Gilman Drive Tata Hall 5401, San 
            Diego, CA 92122, USA
REFERENCE   2  (bases 1 to 7130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2021) Cell and Developmental Biology, University of 
            California, San Diego, 9500 Gilman Drive Tata Hall 5401, San Diego, 
            CA 92122, USA"
FEATURES             Location/Qualifiers
     source          1..7130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(127..715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(889..1746)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1747..1851)
                     /label=AmpR promoter
     CDS             3215..3889
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     CDS             3899..3919
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4060..4181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4184..4604
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3
                     snRNA (Port et al., 2014)"
     ncRNA           4604..4622
                     /label=sgRNA
                     /note="white intron gRNA"
                     /ncRNA_class="sgRNA"
     misc_RNA        4624..4699
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      4706..4933
                     /label=U6 terminal
                     /note="U6 terminal"
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    complement(4999..5128)
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     CDS             complement(5146..5862)
                     /label=mCerulean
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
     regulatory      6040..6083
                     /label=3XP3
                     /note="3XP3"
                     /regulatory_class="promoter"
ORIGIN
        1 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
       61 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      121 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      181 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      241 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      301 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      361 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      421 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      481 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      541 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      601 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      661 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      721 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
      781 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      841 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
      901 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
      961 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1021 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1081 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1141 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1201 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1261 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1321 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1381 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1441 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1501 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1561 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1621 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1681 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1741 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1801 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1861 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     1921 taggcgtatc acgaggccct ttcgggtcat tatttggcta gacgaatgct ttgggctcag
     1981 catctgatat ctaggtatct tcgtgcgtat cttgctttaa attcttagca cctcggcttg
     2041 tataacaaaa taaataagtg agtacgattt gcatatctag ccccgggctc tttgaaacaa
     2101 ttttgaaaag tctcaaaaag ttatacaagg agataagaac tttaattctt ttgggaagta
     2161 agtaacgcag taaaggtaac aaagtattga aaaatatgat atgtatggaa tatttgaagc
     2221 catctttaat tatatgttcg ttgcatatat gtacatattg ggccgtttac gctctgatat
     2281 ttccttaata atatcgagtg gtcgtcagct tttccgctga ataattgcgc ctccttcagt
     2341 atttcgttct catcaaagtg aatgactttt gagtgcactc attagccact cgcacaatcg
     2401 acaaaacggg aggaacaaca aattgagtcg cttctttatt cagtcatttc aatttgtcaa
     2461 gttcatcacg atactccttc gcctccctgc acagcgccat ctagcgccca ctcctcgagt
     2521 ttccttcgta tttcgtaagt aattcaaaaa tagcacgcaa agtaaataaa aaaaaaagta
     2581 agccaaaaaa ttaaataaat atgaaatgta gcatctttcg ctacttattt caacaaaata
     2641 cgaaaatagg gaaaaggtgg gagagtaaac cagaggaggc agggagataa gaaaacccag
     2701 tgggaaaggg acggcgaaaa cagaaaagaa aagaaaactt tttaataaca tttttcggcc
     2761 tcatttgttg tgaaaacaat ttcgccatgt ccctctatct ctttcccagc catatggcca
     2821 tctcgctctg ctcgaggagt gcctgaaaac ggatattaca ttttttcgcg tctacctctc
     2881 gtaacgtcac caattgtgct ttttttttgg taacaggcca aaaactggac actgagtggc
     2941 tagaagaata agctgtcaaa gcatcgcagc agaagatgag cagtgaagcc tcgctagtcc
     3001 atcatgatgg tcgactctag aagtcgatcc aacatggcga cttgtcccat ccccggcatg
     3061 tttaaatata ctaattattc ttgaactaat tttaatcaac cgatttatct ctcttccgca
     3121 ggtgggaggt tccggtggaa gcggaggtag cggcggatcc gagggccgcg gcagcctgct
     3181 gacctgcggc gatgtggagg agaaccccgg gcccatggcc tcctccgaga acgtcatcac
     3241 cgagttcatg cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg agttcgagat
     3301 cgagggcgag ggcgagggcc gcccctacga gggccacaac accgtgaagc tgaaggtgac
     3361 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc
     3421 caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga
     3481 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggcga ccgtgaccca
     3541 ggactcctcc ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt
     3601 cccctccgac ggccccgtga tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     3661 cctgtacccc cgcgacggcg tgctgaaggg cgagacccac aaggccctga agctgaagga
     3721 cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct
     3781 gcccggctac tactacgtgg acgccaagct ggacatcacc tcccacaacg aggactacac
     3841 catcgtggag cagtacgagc gcaccgaggg ccgccaccac ctgttcctga gatctcgacc
     3901 caagaaaaag cggaaggtgg aggacccgta agatccaccg gatctagata actgatcata
     3961 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     4021 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     4081 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4141 cattctagtt gtggtttgtc caaactcatc aatgtatctt accttttttg ctcacctgtg
     4201 attgctccta ctcaaataca aaaacatcaa attttctgtc aataaagcat atttatttat
     4261 atttatttta caggaaagaa ttccttttaa agtgtatttt aacctataat gaaaaacgat
     4321 taaaaaaaat acataaaata attcgaaaat ttttgaatag cccaggttga taaaaattca
     4381 tttcatacgt tttataactt atgcccctaa gtattttttg accatagtgt ttcaattcta
     4441 cattaatttt acagagtaga atgaaacgcc acctactcag ccaagaggcg aaaaggttag
     4501 ctcgccaagc agagagggcg ccagtgctca ctacttttta taattctcaa cttctttttc
     4561 cagactcagt tcgtatatat agacctattt tcaatttaac gtcgcagtga agcctcgcta
     4621 agggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     4681 agtggcaccg agtcggtgct tttttgccta cctggagcct gagagttgtt caataaaata
     4741 aaaatgtttc gtttttttgc tttcgccagt atttattatt tttcatcaat atgtattcaa
     4801 tttggtatgt atttagtaat tgtaatatat agacaatggt tttccgttga cgtacataca
     4861 tctgacgtgt gtttatttag acataatagt tatgttttca catcttttta atgttcgctt
     4921 aatgcgtatg catttagacc tacgccccca actgagagaa ctcaaaggtt accccagttg
     4981 gggcactacg atcgatccca cgcgcttgaa aggagtgtgt aaatggacaa tgttgttaca
     5041 ataaaagttg aaattatttt ataaaattta ttaaaaacat atttaaacat aacatattgt
     5101 attaaaacat gacaatacaa actaagattt agtcagataa acctatttgt agagttcgtc
     5161 catacccaaa gtaattcctg ccgcggtcac gaactccagc aacaccatat gatccctctt
     5221 ttcgttggga tccttactga gtttactctg cgtgctcagg tagtggttgt caggcaagag
     5281 cacgggaccg tcgccgatag gtgtattttg ctgataatga tcggccaatt ggaccgatcc
     5341 atcttctatg ttacaattca gaccgaaatt cgctttgata ccgtttttct gtttatcggc
     5401 tgttatataa acatttccat ggatagcgtt atattccaac ttatggccca gaatattacc
     5461 atcttcctta aagtctatac ccttcaactc gatacgattc accagtgtat caccttcaaa
     5521 cttgacctct gcccgtgttt tgtagttgcc atcgtctttg aaaaatattg tccgttcctg
     5581 cacataacct tctggcattg cacttttaaa gaagtcgtgc tgcttcatgt ggtccgggta
     5641 acgagcgaaa cactgaactc cccacgagag tgtagtcacg agtgtcggcc agggtacagg
     5701 cagtttgccc gtggtgcaaa tgaatttcag ggtcagttta ccgtaagtag catctccttc
     5761 accttctcca ctgacactga atttgtgacc gttcacgtca ccatccaatt cgacgagtat
     5821 cgggaccacg ccagtgaaga gttcttcacc tttactcacc atggtggcga ccggcttcga
     5881 gccgattgtt tagcttgttc agctgcgctt gtttatttgc ttagctttcg cttagcgacg
     5941 tgttcacttt gcttgtttga attgaattgt cgctccgtag acgaagcgcc tctatttata
     6001 ctccggcggt cgagggttcg aaatcgataa gcttggatcc taattgaatt agctctaatt
     6061 gaattagtct ctaattgaat tagatccccg ggcgagctcg aggaggaaat gcaacagttt
     6121 tcaggctttg gtcttttagc tagacataca tatgtagcaa tgtaaacgat aaatccaaat
     6181 cccccaatcg ttttgaaaaa taaatttaat tttaaatagt tcaaaaaatg aaagttctaa
     6241 gcagggctcg ctgtagcaag gaccgccagg cgctgagtgc aaggtggaca ttattttttc
     6301 ttgcttttat tgaatgtcag ctttttattt ggccctcttc ttcggatttt gcctttctca
     6361 tttgcttgca tttttttgct aaattttttt tatgattatt tatacaaatt attatgagag
     6421 cagcggcgga taaaatgctt tccatattgt ggccatcatt ttgtcttgtt ttttccattt
     6481 tatttttgcc tggacgccaa ttagtggatc cctgttattt atgctttgcc gcaggccgct
     6541 gcgatggcaa gaaaccccac aaatggaaaa cggaaaattg tttatgaaaa tgaaaatatg
     6601 cgcgaacgga agtggtatgg ggcgaaaagt tggggcgggg aggatggctt gatttgacca
     6661 taccgcaaac gtcttcggtt accgccattt cgaaaaggga agccccacct gggcgaaaac
     6721 tgacagctca gtgggtttgg aaatcacttt acccgtaaac aaaataacgt aaataagaat
     6781 ttgtatttcg gatttataaa cttaaactca attgcagaag tagtagtttg aacactattg
     6841 cgttcaacat aattggatca agtaaatgga ctgctttata ctgctcattg cacttatcta
     6901 caagagattg agttttccca ccacccaaaa aaagccaaag tatccttaaa gttttatatt
     6961 taataaaaac aaaactggcg aagctcaaag tccccaaacg aaagccaaag atgccagcga
     7021 tttacactcg caattgtctt atgaggtggc aacatggtgg aggaaccttg ccactgccac
     7081 cacccatcgt cccatcgccc catcgtccat cgcccaacga cctgtgaccc
//
