pGABE vector (V016437)

LOCUS       V016437                 7925 bp    DNA     circular SYN 25-MAR-2019
DEFINITION  Exported.
ACCESSION   V016437
VERSION     V016437
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2018) State Key Laboratory of Food Science and
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su
            214122, China
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (28-NOV-2018) State Key Laboratory of Food Science and Technology,
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
FEATURES             Location/Qualifiers
     source          1..7925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(83..126)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     promoter        144..178
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     regulatory      179..190
                     /label="B0034"
                     /note="B0034"
                     /regulatory_class="ribosome_binding_site"
     CDS             199..1248
                     /gene="aroG"
                     /label="Phospho-2-dehydro-3-deoxyheptonate aldolase,
                     Phe-sensitive"
                     /note="Phospho-2-dehydro-3-deoxyheptonate aldolase,
                     Phe-sensitive from Escherichia coli (strain K12).
                     Accession#: P0AB91"
     regulatory      1258..1269
                     /label="B0034"
                     /note="B0034"
                     /regulatory_class="ribosome_binding_site"
     CDS             1278..3266
                     /gene="tktA"
                     /label="Transketolase 1"
                     /note="Transketolase 1 from Escherichia coli (strain K12).
                     Accession#: P27302"
     CDS             3276..3293
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3519..3590
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3606..3633
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     regulatory      3641..3669
                     /label="j23119"
                     /note="j23119"
                     /regulatory_class="promoter"
     regulatory      3676..3687
                     /label="B0034"
                     /note="B0034"
                     /regulatory_class="ribosome_binding_site"
     CDS             3694..4779
                     /gene="aroB"
                     /label="3-dehydroquinate synthase"
                     /note="3-dehydroquinate synthase from Escherichia coli
                     (strain K12). Accession#: P07639"
     regulatory      4789..4800
                     /label="B0034"
                     /note="B0034"
                     /regulatory_class="ribosome_binding_site"
     CDS             4807..5622
                     /gene="aroE"
                     /label="Shikimate dehydrogenase (NADP(+))"
                     /note="Shikimate dehydrogenase (NADP(+)) from Escherichia
                     coli O9:H4 (strain HS). Accession#: A8A586"
     CDS             5632..5649
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      complement(5878..5921)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      5962..5989
                     /label="T7Te terminator"
                     /note="T7Te terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(6194..6782)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6956..7813)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7814..7918)
                     /label="AmpR promoter"