LOCUS       V016437                 7925 bp    DNA     circular SYN 25-MAR-2019
DEFINITION  Exported.
ACCESSION   V016437
VERSION     V016437
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Gao C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2018) State Key Laboratory of Food Science and
            Technology, Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su
            214122, China
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (28-NOV-2018) State Key Laboratory of Food Science and Technology,
            Jiangnan University, 1800 Lihu Road, Wu Xi, Jiang Su 214122, China"
FEATURES             Location/Qualifiers
     source          1..7925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(83..126)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     promoter        144..178
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     regulatory      179..190
                     /label="B0034"
                     /note="B0034"
                     /regulatory_class="ribosome_binding_site"
     CDS             199..1248
                     /gene="aroG"
                     /label="Phospho-2-dehydro-3-deoxyheptonate aldolase,
                     Phe-sensitive"
                     /note="Phospho-2-dehydro-3-deoxyheptonate aldolase,
                     Phe-sensitive from Escherichia coli (strain K12).
                     Accession#: P0AB91"
     regulatory      1258..1269
                     /label="B0034"
                     /note="B0034"
                     /regulatory_class="ribosome_binding_site"
     CDS             1278..3266
                     /gene="tktA"
                     /label="Transketolase 1"
                     /note="Transketolase 1 from Escherichia coli (strain K12).
                     Accession#: P27302"
     CDS             3276..3293
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3519..3590
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3606..3633
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     regulatory      3641..3669
                     /label="j23119"
                     /note="j23119"
                     /regulatory_class="promoter"
     regulatory      3676..3687
                     /label="B0034"
                     /note="B0034"
                     /regulatory_class="ribosome_binding_site"
     CDS             3694..4779
                     /gene="aroB"
                     /label="3-dehydroquinate synthase"
                     /note="3-dehydroquinate synthase from Escherichia coli
                     (strain K12). Accession#: P07639"
     regulatory      4789..4800
                     /label="B0034"
                     /note="B0034"
                     /regulatory_class="ribosome_binding_site"
     CDS             4807..5622
                     /gene="aroE"
                     /label="Shikimate dehydrogenase (NADP(+))"
                     /note="Shikimate dehydrogenase (NADP(+)) from Escherichia
                     coli O9:H4 (strain HS). Accession#: A8A586"
     CDS             5632..5649
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      complement(5878..5921)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      5962..5989
                     /label="T7Te terminator"
                     /note="T7Te terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(6194..6782)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6956..7813)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7814..7918)
                     /label="AmpR promoter"
ORIGIN
        1 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
       61 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
      121 gatttctgga attctaaaga tctttgacag ctagctcagt cctaggtata atactagtaa
      181 agaggagaaa aagcttggat gaattatcag aacgacgatt tacgcatcaa agaaatcaaa
      241 gagttacttc ctcctgtcgc attgctggaa aaattccccg ctactgaaaa tgccgcgaat
      301 acggttgccc atgcccgaaa agcgatccat aagatcctga aaggtaatga tgatcgcctg
      361 ttggttgtga ttggcccatg ctcaattcat gatcctgtcg cggcaaaaga gtatgccact
      421 cgcttgctgg cgctgcgtga agagctgaaa gatgagctgg aaatcgtaat gcgcgtctat
      481 tttgaaaagc cgcgtaccac ggtgggctgg aaagggctga ttaacgatcc gcatatggat
      541 aatagcttcc agatcaacga cggtctgcgt atagcccgta aattgctgct tgatattaac
      601 gacagcggtc tgccagcggc aggtgagttt ctcaatatga tcaccccaca atatctcgct
      661 gacctgatga gctggggcgc aattggcgca cgtaccaccg aatcgcaggt gcaccgcgaa
      721 ctggcatcag ggctttcttg tccggtcggc ttcaaaaatg gcaccgacgg tacgattaaa
      781 gtggctatcg atgccattaa tgccgccggt gcgccgcact gcttcctgtc cgtaacgaaa
      841 tgggggcatt cggcgattgt gaataccagc ggtaacggcg attgccatat cattctgcgc
      901 ggcggtaaag agcctaacta cagcgcgaag cacgttgctg aagtgaaaga agggctgaac
      961 aaagcaggcc tgccagcaca ggtgatgatc gatttcagcc atgctaactc gtccaaacaa
     1021 ttcaaaaagc agatggatgt ttgtgctgac gtttgccagc agattgccgg tggcgaaaag
     1081 gccattattg gcgtgatggt ggaaagccat ctggtggaag gcaatcagag cctcgagagc
     1141 ggggagccgc tggcctacgg taagagcatc accgatgcct gcatcggctg ggaagatacc
     1201 gatgctctgt tacgtcaact ggcgaatgca gtaaaagcgc gtcgcgggta aactggtaaa
     1261 gaggagaaaa agcttggatg tcctcacgta aagagcttgc caatgctatt cgtgcgctga
     1321 gcatggacgc agtacagaaa gccaaatccg gtcacccggg tgcccctatg ggtatggctg
     1381 acattgccga agtcctgtgg cgtgatttcc tgaaacacaa cccgcagaat ccgtcctggg
     1441 ctgaccgtga ccgcttcgtg ctgtccaacg gccacggctc catgctgatc tacagcctgc
     1501 tgcacctcac cggttacgat ctgccgatgg aagaactgaa aaacttccgt cagctgcact
     1561 ctaaaactcc gggtcacccg gaagtgggtt acaccgctgg tgtggaaacc accaccggtc
     1621 cgctgggtca gggtattgcc aacgcagtcg gtatggcgat tgcagaaaaa acgctggcgg
     1681 cgcagtttaa ccgtccgggc cacgacattg tcgaccacta cacctacgcc ttcatgggcg
     1741 acggctgcat gatggaaggc atctcccacg aagtttgctc tctggcgggt acgctgaagc
     1801 tgggtaaact gattgcattc tacgatgaca acggtatttc tatcgatggt cacgttgaag
     1861 gctggttcac cgacgacacc gcaatgcgtt tcgaagctta cggctggcac gttattcgcg
     1921 acatcgacgg tcatgacgcg gcatctatca aacgcgcagt agaagaagcg cgcgcagtga
     1981 ctgacaaacc ttccctgctg atgtgcaaaa ccatcatcgg tttcggttcc ccgaacaaag
     2041 ccggtaccca cgactcccac ggtgcgccgc tgggcgacgc tgaaattgcc ctgacccgcg
     2101 aacaactggg ctggaaatat gcgccgttcg aaatcccgtc tgaaatctat gctcagtggg
     2161 atgcgaaaga agcaggccag gcgaaagaat ccgcatggaa cgagaaattc gctgcttacg
     2221 cgaaagctta tccgcaggaa gccgctgaat ttacccgccg tatgaaaggc gaaatgccgt
     2281 ctgacttcga cgctaaagcg aaagagttca tcgctaaact gcaggctaat ccggcgaaaa
     2341 tcgccagccg taaagcgtct cagaatgcta tcgaagcgtt cggtccgctg ttgccggaat
     2401 tcctcggcgg ttctgctgac ctggcgccgt ctaacctgac cctgtggtct ggttctaaag
     2461 caatcaacga agatgctgcg ggtaactaca tccactacgg tgttcgcgag ttcggtatga
     2521 ccgcgattgc taacggtatc tccctgcacg gtggcttcct gccgtacacc tccaccttcc
     2581 tgatgttcgt ggaatacgca cgtaacgccg tacgtatggc tgcgctgatg aaacagcgtc
     2641 aggtgatggt ttacacccac gactccatcg gtctgggcga agacggcccg actcaccagc
     2701 cggttgagca ggtcgcttct ctgcgcgtaa ccccgaacat gtctacatgg cgtccgtgtg
     2761 accaggttga atccgcggtc gcgtggaaat acggtgttga gcgtcaggac ggcccgaccg
     2821 cactgatcct ctcccgtcag aacctggcgc agcaggaacg aactgaagag caactggcaa
     2881 acatcgcgcg cggtggttat gtgctgaaag actgcgccgg tcagccggaa ctgattttca
     2941 tcgctaccgg ttcagaagtt gaactggctg ttgctgccta cgaaaaactg actgccgaag
     3001 gcgtgaaagc gcgcgtggtg tccatgccgt ctaccgacgc atttgacaag caggatgctg
     3061 cttaccgtga atccgtactg ccgaaagcgg ttactgcacg cgttgctgta gaagcgggta
     3121 ttgctgacta ctggtacaag tatgttggcc tgaacggtgc tatcgtcggt atgaccacct
     3181 tcggtgaatc tgctccggca gagctgctgt ttgaagagtt cggcttcact gttgataacg
     3241 ttgttgcgaa agcaaaagaa ctgctgtaag tcgaccatca tcatcatcat cattgagttt
     3301 aaacggtctc cagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat
     3361 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
     3421 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3481 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     3541 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     3601 acactggctc accttcgggt gggcctttct gcgtggatct ttgacagcta gctcagtcct
     3661 aggtataata ctagtaaaga ggagaaaaag cttatggagc gtattgtcgt tactctcggg
     3721 gaacgtagtt acccaattac catcgcatct ggtttgttta atgaaccagc ttcattctta
     3781 ccgctgaaat cgggcgagca ggtcatgttg gtcaccaacg aaaccctggc tcctctgtat
     3841 ctcgataagg tccgcggcgt acttgaacag gcgggtgtta acgtcgatag cgttatcctc
     3901 cctgacggcg agcagtataa aagcctggct gtactcgata ccgtctttac ggcgttgtta
     3961 caaaaaccgc atggtcgcga tactacgctg gtggcgcttg gcggcggcgt agtgggcgat
     4021 ctgaccggct tcgcggcggc gagttatcag cgcggtgtcc gtttcattca agtcccgacg
     4081 acgttactgt cgcaggtcga ttcctccgtt ggcggcaaaa ctgcggtcaa ccatcccctc
     4141 ggtaaaaaca tgattggcgc gttctaccaa cctgcttcag tggtggtgga tctcgactgt
     4201 ctgaaaacgc ttcccccgcg tgagttagcg tcggggctgg cagaagtcat caaatacggc
     4261 attattcttg acggtgcgtt ttttaactgg ctggaagaga atctggatgc gttgttgcgt
     4321 ctggacggtc cggcaatggc gtactgtatt cgccgttgtt gtgaactgaa ggcagaagtt
     4381 gtcgccgccg acgagcgcga aaccgggtta cgtgctttac tgaatctggg acacaccttt
     4441 ggtcatgcca ttgaagctga aatggggtat ggcaattggt tacatggtga agcggtcgct
     4501 gcgggtatgg tgatggcggc gcggacgtcg gaacgtctcg ggcagtttag ttctgccgaa
     4561 acgcagcgta ttataaccct gctcaagcgg gctgggttac cggtcaatgg gccgcgcgaa
     4621 atgtccgcgc aggcgtattt accgcatatg ctgcgtgaca agaaagtcct tgcgggagag
     4681 atgcgcttaa ttcttccgtt ggcaattggt aagagtgaag ttcgcagcgg cgtttcgcac
     4741 gagcttgttc ttaacgccat tgccgattgt caatcagcgt aagagctcaa agaggagaaa
     4801 aagcttatgg aaacctatgc tgtttttggt aatccgatag cccacagcaa atcgccattc
     4861 attcatcagc aatttgctca gcaactgaat attgaacatc cctatgggcg cgtgttggca
     4921 cccatcaatg atttcatcaa cacactgaac gctttcttta gtgctggtgg taaaggtgcg
     4981 aatgtgacgg tgccttttaa agaagaggct tttgccagag cggatgagct tactgaacgg
     5041 gcagcgttgg ctggtgctgt taataccctc atgcggttag aagatggacg cctgctgggt
     5101 gacaataccg atggtgtagg cttgttaagc gatctggaac gtctgtcttt tatccgccct
     5161 ggtttacgta ttctgcttat cggcgctggt ggagcatctc gcggcgtact actgccactc
     5221 ctttccctgg actgtgcggt gacaataact aatcggacgg tatcccgcgc ggaagagttg
     5281 gctaaattgt ttgcgcacac tggcagtatt caggcgttga gtatggacga actggaaggt
     5341 catgagtttg atctcattat taatgcaaca tccagtggca tcagtggtga tattccggcg
     5401 atcccgtcat cgctcattca tccaggcatt tattgctatg acatgttcta tcagaaagga
     5461 aaaactcctt ttctggcatg gtgtgagcag cgaggctcaa agcgtaatgc tgatggttta
     5521 ggaatgctgg tggcacaggc ggctcatgcc tttcttctct ggcacggtgt tctgcctgac
     5581 gtagaaccag ttataaagca attgcaggag gaattgtccg cgtgagtcga ccatcatcat
     5641 catcatcatt gagtttaaac ggtctccagc ttggctgttt tggcggatga gagaagattt
     5701 tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg
     5761 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
     5821 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata
     5881 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     5941 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ggatccttac
     6001 tcgagtctag actgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6061 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6601 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6961 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7021 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7081 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7141 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7201 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7261 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7321 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7381 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7441 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7501 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7561 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7621 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7681 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7741 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7801 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7861 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7921 cattt
//
