Basic Vector Information
- Vector Name:
- pNRVL-
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3295 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Nemeth T.
- Promoter:
- T7
pNRVL- vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNRVL- vector Sequence
LOCUS 62056_18200 3295 bp DNA circular SYN 03-MAR-2020
DEFINITION Cloning vector pNRVL', complete sequence.
ACCESSION MN956532
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3295)
AUTHORS Nemeth T.
TITLE Direct Submission
JOURNAL Submitted (16-JAN-2020) Department of Microbiology, University of
Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE 2 (bases 1 to 3295)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(16-JAN-2020) Department of Microbiology, University of Szeged,
Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT ##Assembly-Data-START##
Assembly Method :: CLC Genomics Workbench v. v11
Sequencing Technology :: IonTorrent
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..3295
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 239..244
/label=NheI site
/note="NheI site"
terminator complement(268..295)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(387..473)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 505..510
/label=NheI site
/note="NheI site"
primer_bind 537..553
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 570..575
/label=StuI site for cassette release
/note="StuI site for cassette release"
misc_structure 576..1039
/note="Site of recombination; CpNEUT5LUpup from C.
parapsilosis"
misc_feature 1040..1047
/label=NotI site for selection marker ligation
/note="NotI site for selection marker ligation"
misc_feature 1054..1059
/note="BssHII site for selection marker and insert
ligation"
misc_feature 1067..1072
/label=XhoI site for insert ligation
/note="XhoI site for insert ligation"
misc_structure 1073..1523
/note="Site of recombination; CpNEUT5LDowndown from C.
parapsilosis"
promoter complement(1537..1555)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1560..1576)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
regulatory 1590..1688
/label=BBa_P1007 promoter
/note="BBa_P1007 promoter"
/regulatory_class="promoter"
CDS 1689..2495
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 2645..3233
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
This page is informational only.