LOCUS       62056_18200        3295 bp DNA     circular SYN 03-MAR-2020
DEFINITION  Cloning vector pNRVL', complete sequence.
ACCESSION   MN956532
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2020) Department of Microbiology, University of 
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (16-JAN-2020) Department of Microbiology, University of Szeged, 
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    239..244
                     /label=NheI site
                     /note="NheI site"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    505..510
                     /label=NheI site
                     /note="NheI site"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    570..575
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     misc_structure  576..1039
                     /note="Site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     misc_feature    1040..1047
                     /label=NotI site for selection marker ligation
                     /note="NotI site for selection marker ligation"
     misc_feature    1054..1059
                     /note="BssHII site for selection marker and insert
                     ligation"
     misc_feature    1067..1072
                     /label=XhoI site for insert ligation
                     /note="XhoI site for insert ligation"
     misc_structure  1073..1523
                     /note="Site of recombination; CpNEUT5LDowndown from C. 
                     parapsilosis"
     promoter        complement(1537..1555)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1560..1576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1590..1688
                     /label=BBa_P1007 promoter
                     /note="BBa_P1007 promoter"
                     /regulatory_class="promoter"
     CDS             1689..2495
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2645..3233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccta ggccttgaat tggattggga ttagattagg
      601 atttgatggg tttttttttt tctgttaaat ggttatataa tcttcgtttc tgtgagatgt
      661 tttttttttt ctaaattaaa attgtattat gtaatggatt atgtaatttt gcattgcatt
      721 ttttagttat agtagtagta atagtagtac tgcatataca aacacactat tgtagatgag
      781 ggtagtgtac tattgcacaa atttggttta ctcctatttc gcatcattgc agttcatggt
      841 ttgtatgcag taatatatat ttatctctat ttctgtctct cctaagacca gtacaactta
      901 ccactcaatc acacgatata gtctacgagg ctacttcttt atctaccact ggcaatataa
      961 tggtaaagag agcttttccc aagaaccagg tgtggaataa tgaaaagtct atctatggaa
     1021 ggatacatgt tctagatctg cggccgctgc agtgcgcgct acggatctcg agcgaagtag
     1081 tcctcactga ggagatccca ggctagaaat cagcaaattt tacatccctc atattctgac
     1141 acgagtttaa ctacatagca attgtaaaag ggctcttgtt tttttttttt ttttgtggcg
     1201 gtgcagtacg tacacgatct ttaaagatta gggctataac catgtcaaag tgactcattt
     1261 tttttgttca aatgcgcact cggatttaaa gcagcaaaaa aatacttcgt tacaaaaatg
     1321 gaagcgcatt ttccgaaaaa ggttaattcc tagcgatttt attatttttg ttttatttat
     1381 taatttttct tgttttgcta ttgcattttt gcaaccaaaa atagaaaatt ctcgtttttt
     1441 tttgttgtag cgcaaccaat ttctggttat tctttttttt ttgcaatttt taaatttgca
     1501 cgtgacagtg ttagtgttag tgcaggcctg atatccccta tagtgagtcg tattacatgg
     1561 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1621 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1681 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     1741 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1801 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1861 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     1921 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     1981 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2041 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2101 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2161 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2221 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2281 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2341 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2401 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2461 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     2521 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     2581 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
