NAND_Reporter vector (V016403)

MSDS

Basic Vector Information

Vector Name:: NAND_Reporter

Antibiotic Resistance:: Kanamycin

Length:: 5173 bp

Type:: Cloning vector

Replication origin:: pSC101 ori

Source/Author:: Groseclose TM, Rondon RE, Herde ZD, Aldrete CA

NAND_Reporter vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

NAND_Reporter vector Sequence

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LOCUS       62056_1730        5173 bp DNA     circular SYN 10-SEP-2020
DEFINITION  Cloning vector NAND_Reporter, complete sequence.
ACCESSION   MT127260
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Engineered systems of inducible anti-repressors for the next 
            generation of biological programming
  JOURNAL   Nat Commun 11 (1), 4440 (2020)
  PUBMED    32895374
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5173)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4440"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-FEB-2020) Chemical "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      51..56
                     /label=trc promoter -35 hexamer
                     /note="trc promoter -35 hexamer"
                     /regulatory_class="promoter"
     regulatory      57..73
                     /label=operator Ottg
                     /note="operator Ottg"
                     /regulatory_class="other"
     regulatory      74..79
                     /label=trc promoter -10 hexamer
                     /note="trc promoter -10 hexamer"
                     /regulatory_class="promoter"
     regulatory      95..114
                     /label=operator Otta
                     /note="operator Otta"
                     /regulatory_class="other"
     regulatory      131..205
                     /label=RiboJ10
                     /note="RiboJ10"
                     /regulatory_class="insulator"
     regulatory      224..235
                     /label=strong bacterial RBS
                     /note="strong bacterial RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             242..955
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     terminator      970..1056
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1261..2208)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(2256..2478)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     regulatory      2565..2570
                     /label=pLL promoter -35 hexamer
                     /note="pLL promoter -35 hexamer"
                     /regulatory_class="promoter"
     regulatory      2571..2587
                     /label=operator Oagg
                     /note="operator Oagg"
                     /regulatory_class="other"
     regulatory      2588..2593
                     /label=pLL promoter -10 hexamer
                     /note="pLL promoter -10 hexamer"
                     /regulatory_class="promoter"
     regulatory      2609..2628
                     /label=operator Otta
                     /note="operator Otta"
                     /regulatory_class="other"
     misc_RNA        2646..2696
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      2698..2719
                     /label=RiboJ
                     /note="RiboJ"
                     /regulatory_class="insulator"
     regulatory      2724..2735
                     /label=strong bacterial RBS
                     /note="strong bacterial RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             2742..3455
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     terminator      complement(3474..3517)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(4118..4212)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4246..5037)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"

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