LOCUS       62056_1730        5173 bp DNA     circular SYN 10-SEP-2020
DEFINITION  Cloning vector NAND_Reporter, complete sequence.
ACCESSION   MT127260
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Engineered systems of inducible anti-repressors for the next 
            generation of biological programming
  JOURNAL   Nat Commun 11 (1), 4440 (2020)
  PUBMED    32895374
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Groseclose TM, Rondon RE, Herde ZD, Aldrete CA, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5173)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "4440"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-FEB-2020) Chemical "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      51..56
                     /label=trc promoter -35 hexamer
                     /note="trc promoter -35 hexamer"
                     /regulatory_class="promoter"
     regulatory      57..73
                     /label=operator Ottg
                     /note="operator Ottg"
                     /regulatory_class="other"
     regulatory      74..79
                     /label=trc promoter -10 hexamer
                     /note="trc promoter -10 hexamer"
                     /regulatory_class="promoter"
     regulatory      95..114
                     /label=operator Otta
                     /note="operator Otta"
                     /regulatory_class="other"
     regulatory      131..205
                     /label=RiboJ10
                     /note="RiboJ10"
                     /regulatory_class="insulator"
     regulatory      224..235
                     /label=strong bacterial RBS
                     /note="strong bacterial RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             242..955
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     terminator      970..1056
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1261..2208)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(2256..2478)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     regulatory      2565..2570
                     /label=pLL promoter -35 hexamer
                     /note="pLL promoter -35 hexamer"
                     /regulatory_class="promoter"
     regulatory      2571..2587
                     /label=operator Oagg
                     /note="operator Oagg"
                     /regulatory_class="other"
     regulatory      2588..2593
                     /label=pLL promoter -10 hexamer
                     /note="pLL promoter -10 hexamer"
                     /regulatory_class="promoter"
     regulatory      2609..2628
                     /label=operator Otta
                     /note="operator Otta"
                     /regulatory_class="other"
     misc_RNA        2646..2696
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      2698..2719
                     /label=RiboJ
                     /note="RiboJ"
                     /regulatory_class="insulator"
     regulatory      2724..2735
                     /label=strong bacterial RBS
                     /note="strong bacterial RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             2742..3455
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     terminator      complement(3474..3517)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(4118..4212)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4246..5037)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacgtccaac atatggcacc ggaggctttc gtcttcacct cgagaattgt ttgacatttt
       61 gagcgctcaa aattataatt cgtgcacttc gtgcaatttt aagcgcttaa aattggactg
      121 cttgcacctg agcgctcaac gggtgtgctt cccgttctga tgagtccgtg aggacgaaag
      181 cgcctctaca aataattttg tttaactgag cacagaattc attaaagagg agaaaggtac
      241 catgagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      301 tgatgttaat gggcacaaat tttctgtccg tggagagggt gaaggtgatg ctacaaacgg
      361 aaagcttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      421 tgtcactact ctcacttatg gtgttcaatg cttttcccgt tatccggatc atatgaaacg
      481 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt
      541 caaagatgac gggacttaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt
      601 taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc tcggacacaa
      661 acttgagtac aactttaact cacacaatgt atacatcacg gcagacaaac aaaagaatgg
      721 aatcaaagct aacttcaaaa ttcgccacaa cgttgaagat ggatccgttc aactagcaga
      781 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      841 cctgtcgaca caatctgtcc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct
      901 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaataatc
      961 tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1021 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac ctagggtacg
     1081 ggttttgctg cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga
     1141 tccggcttca gccggtttgc cggctgaaag cgctatttct tccagaattg ccatgatttt
     1201 ttccccacgg gaggcgtcac tggctcccgt gttgtcggca gctttgattc gataagctca
     1261 gatccttccg tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca
     1321 tgagaacgaa ccattgagat catgcttact ttgcatgtca ctcaaaaatt ttgcctcaaa
     1381 actggtgagc tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttacg
     1441 taggtaggaa tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt
     1501 gttctcaagt tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt
     1561 atcagtcggg cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc
     1621 tttacttatt ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc
     1681 aagcattaac atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt
     1741 cttttgtgtt agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa
     1801 agacttaaca tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa
     1861 tatctcttca ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca
     1921 ctggaaaatc tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag
     1981 ctctctggtt gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg
     2041 agcgtattgg ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt
     2101 ggggttgagt agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata
     2161 gcgactaatc gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg
     2221 gtctaggtga ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt
     2281 ccttttcccg ggagatctgg gtatctgtaa attctgctag acctttgctg gaaaacttgt
     2341 aaattctgct agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat
     2401 tcaagtggtt ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc
     2461 cagccctgtg tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca
     2521 aacgctgttt gctcctctac aaaacagacc ttaaaaccct aaagttgaca ttaggagcgc
     2581 tcctaatgat actatatagt gaacaaggaa ttttaagcgc ttaaaattga ttcctgttag
     2641 gatcagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc
     2701 tacaaataat tttgtttaac ctcaaagagg agaaactcga catgagcaaa ggagaagaac
     2761 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     2821 tttctgtccg tggagagggt gaaggtgatg ctacaaacgg aaagcttacc cttaaattta
     2881 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ctcacttatg
     2941 gtgttcaatg cttttcccgt tatccggatc atatgaaacg gcatgacttt ttcaagagtg
     3001 ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac gggacttaca
     3061 agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc gagttaaaag
     3121 gtattgattt taaagaagat ggaaacattc tcggacacaa acttgagtac aactttaact
     3181 cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa
     3241 ttcgccacaa cgttgaagat ggatccgttc aactagcaga ccattatcaa caaaatactc
     3301 caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca caatctgtcc
     3361 tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt gtaactgctg
     3421 ctgggattac acatggcatg gatgagctct acaaataaat ctagaggcat caaataaaac
     3481 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     3541 tcctgagtag gacaaatccg ccgccctaga cctagggtac gggttttgct gcccgcaaac
     3601 gggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggcttc agccggtttg
     3661 ccggctgaaa gcgctatttc ttccagaatt gccatgattt tttccccacg ggaggcgtgt
     3721 agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga ccatcaggca
     3781 cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc tggcagtgaa
     3841 tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac tacccataat
     3901 acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc
     3961 tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct gaccacttcg
     4021 gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca gcggtatcat
     4081 caacaggctt acccgtctta ctgtccctag tgcttggatt ctcaccaata aaaaacgccc
     4141 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     4201 tcaacaggag tccaagcgag ctctcgaacc ccagagtccc gctcagaaga actcgtcaag
     4261 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     4321 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     4381 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     4441 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
     4501 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     4561 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     4621 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     4681 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     4741 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     4801 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag
     4861 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     4921 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     4981 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg
     5041 aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg
     5101 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     5161 agctggcaat tcc
//
