Basic Vector Information
- Vector Name:
- pCW619-LM-dat
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8201 bp
- Type:
- UNVERIFIED: Cloning vector
- Replication origin:
- ori
- Source/Author:
- Yuzhen Z.
pCW619-LM-dat vector Map
pCW619-LM-dat vector Sequence
LOCUS V016386 8201 bp DNA circular SYN 20-JUN-2020
DEFINITION Exported.
ACCESSION V016386
VERSION V016386
KEYWORDS UNVERIFIED
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 8201)
AUTHORS Yuzhen Z.
TITLE Direct Submission
JOURNAL Submitted (02-OCT-2019) Department of Public Health Laboratory
Sciences, West China School of Public HealthSichuan University, No.
17, Section 3, Renmin South Road, Wuhou District, Chengdu, Sichuan
610041, China
REFERENCE 2 (bases 1 to 8201)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(02-OCT-2019) Department of Public Health Laboratory Sciences, West
China School of Public HealthSichuan University, No. 17, Section 3,
Renmin South Road, Wuhou District, Chengdu, Sichuan 610041, China"
GenBank staff is unable to verify sequence and/or annotation
provided by the submitter.
FEATURES Location/Qualifiers
source 1..8201
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 515..703
/label="rop"
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 808..948
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(1134..1722)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1896..2753)
/label="AmpR"
/note="beta-lactamase"
promoter complement(2754..2858)
/label="AmpR promoter"
CDS 5881..6612
/gene="ermC"
/label="rRNA adenine N-6-methyltransferase"
/note="rRNA adenine N-6-methyltransferase from
Staphylococcus aureus. Accession#: P02979"
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