LOCUS       V016386                 8201 bp    DNA     circular SYN 20-JUN-2020
DEFINITION  Exported.
ACCESSION   V016386
VERSION     V016386
KEYWORDS    UNVERIFIED
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Yuzhen Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2019) Department of Public Health Laboratory
            Sciences, West China School of Public HealthSichuan University, No.
            17, Section 3, Renmin South Road, Wuhou District, Chengdu, Sichuan
            610041, China
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (02-OCT-2019) Department of Public Health Laboratory Sciences, West
            China School of Public HealthSichuan University, No. 17, Section 3,
            Renmin South Road, Wuhou District, Chengdu, Sichuan 610041, China"
            GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..8201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             515..703
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    808..948
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1134..1722)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1896..2753)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2754..2858)
                     /label="AmpR promoter"
     CDS             5881..6612
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
ORIGIN
        1 tcatgtttga cagcttatca tcgtcgggca gcgttgggtc ctggccacgg gtgcgcatga
       61 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
      121 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
      181 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
      241 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
      301 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
      361 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
      421 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
      481 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct
      541 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct
      601 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta
      661 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
      721 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      781 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
      841 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
      901 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
      961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1561 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     2221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     2521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2881 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2941 aggccctttc gtcttcaaga attcccagca tggagccaca caactgtcgg cggccttcca
     3001 attactgaat ggattagcga agatgaacaa ggtgcaatgg atactatttt cgtaagtgtt
     3061 cgtgatgcag cttatgaaat tattaataaa aaaggcgcta cattctacgg cgttgctgca
     3121 gctcttgctc gtattacaaa agcaattcta aataacgaaa atgcgatttt gccactttct
     3181 gtttatttag atggccatta cggtatgaac gatatttata taggtgcacc agcagtcgtt
     3241 aaccgtcaag gcgttcgcca tattgttgaa atgaacttaa atgacaaaga aaaagaacaa
     3301 atgaaaaact ccgcagatac acttaaaaaa gttctagagt aatcatcgcg aataagtaaa
     3361 gctgacgcgt tgtaaatcgt tcaattaaaa aggccgtcat tggaatcatg acaccattaa
     3421 ctaacataaa tccagttgat aaccattgtc cttgactggc tgtaattcca aaatctttca
     3481 taatacttgg tagcgcgaca ttcatcaacg tttggttgag aatggtaaca aaggcgccca
     3541 taagcataac aatcaaaata ccgttacgct tcactgatgt acttgctgct ttcatattca
     3601 atctcgtgca ccttcccttt ccaaaatggt aataatttct tggtgcattt ttagcatttc
     3661 ttttaaccga tcctcgccaa tctctaagat aggttctaaa cggttgaaga atacatgata
     3721 cgtttctgct gcttttgtag ctcctttttc agttaattct aagctgagtg cgcgacgatt
     3781 attttcattt cttgtacgct ttaaaaatcc cgcttttact aatcgatcta caataccaga
     3841 tacagtactt ttaccaagct tcattcgttc tgctaattcg ccaagtgtta attctgattc
     3901 tctttgtaat tctctgattg caagtaattg ggttgtagta atctctcggt tcgcagcttc
     3961 ttcagctagc acgtgatgtg tttttcgttg tacttcgcga aaagagaaaa tgacctcttt
     4021 caccaatttt tcatccatga cttccctcca tattgttcgc tctcaaatat ttcgtatgcg
     4081 aactattagc cttttgaatt atacaccctg taaaaaattt gtcaatcgct ttaaatggca
     4141 cttttaagta tgaaagcgac accaattgag tattttcaac gaataaaaaa gttatataaa
     4201 tttttacata ggtatcaaag cacaaaaaac cttcctgaaa taggaaggtt ttctactatg
     4261 ctcgagtctc tccttgcata gttaattttt ggaactatct aaaaacttaa tgaaacaaag
     4321 aaactgccgc aaaactaagc tttgcagcag ttaatttttt caattataac ccttacttcg
     4381 ctaatttgta aagcgcttct gcgtaaattg ctgtagcttt taacaaatca tcaaaatagc
     4441 tgaattcgtc tttttggtgc atcgtgtctt cacggcctgg gaatagcgct ccgaacgcta
     4501 cgccagtctc catatgacgt gcataagttc cgccacctat tgctagtaaa gtagcctctt
     4561 cacctgtttg ttttgtatat acttcttgta aaacttgaat aagtgggtga tcttttggta
     4621 cgaaaagtgg tttggaatct tcataatgtg tatattgcgc attatactca tatacgactg
     4681 tttgcatttt atttttaagt ttgtccatgc tagcagtaac tggataacgg aaattaagtc
     4741 catatttgcc accttctgca acatcataac ggataacgcc aacgttcatt gttagttcgc
     4801 cgctttctgc atcttcatag ctaatgccta gtttaacagc acgagaatca ccaaacagat
     4861 agtcacgacc aaatgttacg aaatcattcg ctgctccagt cagcttgaat ttaccaagga
     4921 aagcgactaa atgtagacca gcgttcacac cgttatttgg ttccattgca tgggcagatt
     4981 tgccgacaat attaatttta acggacttgc cattttcttc tagtgttcct tctactggat
     5041 gatttgctag gaaggttttg aaggcactcg ctactttatc aaagtctttt acatcttcta
     5101 aaatagctgt tgcgtgatct ggaaccatgt tgtagcgttc gcctgattct acgctaagta
     5161 aacggaatgc tgcttccccg cctgcttcac cgtctttaaa tgatacatct agttctgaga
     5221 actagtcccg gggcgaattg catctgaccg aattttacgt ttccctgaat aattctcatc
     5281 aatcgtttca tcaattttat ctttatactt tatattttgt gcgttaatca aatcataatt
     5341 tttatatgtt tcctcatgat ttatgtcttt attattatag tttttattct ctctttgatt
     5401 atgtctttgt atcccgtttg tattacttga tcctttaact ctggcaaccc tcaaaattga
     5461 atgagacatg ctacacctcc ggataataaa tatatataaa cgtataaaga tttcataaag
     5521 tctaacacac tagacttatt tacttcgtaa ttaagtcgtt aaaccgtgtg ctctacgacc
     5581 aaaactataa aacctttaag aactttcttt ttttacaaga aaaaagaaat tagataaatc
     5641 tctcatatct tttattcaat aatcgcatcc gattgcagta taaatttaac gatcactcat
     5701 catgttcata tttatcagag ctcgtgctat aattatacta attttataag gaggaaaaaa
     5761 tatgggcatt tttagtattt ttgtaatcag cacagttcat tatcaaccaa acaaaaaata
     5821 agtggttata atgaatcgtt aataagcaaa attcatataa ccaaattaaa gagggttata
     5881 atgaacgaga aaaatataaa acacagtcaa aactttatta cttcaaaaca taatatagat
     5941 aaaataatga caaatataag attaaatgaa catgataata tctttgaaat cggctcagga
     6001 aaaggccatt ttacccttga attagtaaag aggtgtaatt tcgtaactgc cattgaaata
     6061 gaccataaat tatgcaaaac tacagaaaat aaacttgttg atcacgataa tttccaagtt
     6121 ttaaacaagg atatattgca gtttaaattt cctaaaaacc aatcctataa aatatatggt
     6181 aatatacctt ataacataag tacggatata atacgcaaaa ttgttcttga tagtatagct
     6241 aatgagattt atttaatcgt ggaatacggg tttgctaaaa gattattaaa tacaaaacgc
     6301 tcattggcat tacttttaat ggcagaagtt gatatttcta tattaagtat ggttccaaga
     6361 gaatattttc atcctaaacc taaagtgaat agctcactta tcagattaag tagaaaaaaa
     6421 tcaagaatat cacacaaaga taaacaaaag tataattatt tcgttctgaa atgggttaac
     6481 aaagaataca agaaaatatt tacaaaaaat caatttaaca attccttaaa acatgcagga
     6541 attgacgatt taaacaatat tagctttgaa caattcttat ctcttgtcaa tagctataaa
     6601 ttatttaata agtaagttaa gggatgcata aactgcatcc cttaacttgt ttttcgtgtg
     6661 cctatttttt gtgaatcgtc gacaagcttt aatgcggtag tttatcacag ttaaattgct
     6721 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     6781 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     6841 atcgatagaa agcgtgagaa acagcgtaca gacgatttag agatgtagag gtacttttat
     6901 gccgagaaaa ctttttgcgt gtgacagtcc ttaaaatata cttagagcgt aagcgaaagt
     6961 agtagcgaca gctattaact ttcggttgca aagctctagg atttttaatg gacgcagcgc
     7021 atcacacgca aaaaggaaat tggaataaat gcgaaatttg agatgttaat taaagacctt
     7081 tttgaggtct ttttttctta gatttttggg gttatttagg ggagaaaaca taggggggta
     7141 ctacgacctc ccccctaggt gtccattgtc cattgtccaa acaaataaat aaatattggg
     7201 tttttaatgt taaaaggttg ttttttatgt taaagtgaaa aaaacagatg ttgggaggta
     7261 cagtgatagt tgtagataga aaagaagaga aaaaagttgc tgttacttta agacttacaa
     7321 cagaagaaaa tgagatatta aatagaatca aagaaaaata taatattagc aaatcagatg
     7381 caaccggtat tctaataaaa aaatatgcaa aggaggaata cggtgcattt taaacaaaaa
     7441 aagatagaca gcactggcat gctgcctatc tatgactaaa ttttgttaag tgtattagca
     7501 ccgttattat atcatgagcg aaaatgtaat aaaagaaact gaaaacaaga aaaattcaag
     7561 aggacgtaat tggacatttg ttttatatcc agaatcagca aagccgagtg gttagagtat
     7621 ttaaaagagt tacacattca atttgtagtg ctccattaca tgatagggat actgatacag
     7681 aaggtaggat gaaaaaagag cattatcata ttctagtgat gtatgagggt aataaatctt
     7741 atgaacagat aaaaataata acagaagaat tgaatgcgac tattccgcag attgcaggaa
     7801 gtgtgaaagg tcttgtgaga tatatgcttc acatggacga tcctaataaa tttaaatatc
     7861 aaaaagaaga tatgatagtt tatggcggtg tagatgttga tgaattatta aagaaaacaa
     7921 caacagatag atataaatta attaaagaaa tgattgagtt tattgatgaa caaggaatcg
     7981 tagaatttaa gagtttaatg gattatgcaa tgaagtttaa atttgatgat tggttcccgc
     8041 ttttatgtga taactcggcg tatgttattc aagaatatat aaaatcaaat cggtataaat
     8101 ctgaccgata gattttgaat ttaggtgtca caagacactc ttttttcgca ccagcgaaaa
     8161 ctggtttaag ccgagtgcgc aaaagacata atcgggaatt c
//
