pGHKW2 vector (V016378)

LOCUS       V016378                 9425 bp    DNA     circular SYN 03-JUL-2019
DEFINITION  Exported.
ACCESSION   V016378
VERSION     V016378
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Chenyan H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2019) Fujian Agriculture and Forestry University,
            College of Forestry, No. 15 Shangdian Road, Cangshan District,
            Fuzhou, Fujian 350002, China
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2019) Fujian Agriculture and Forestry University, College of
            Forestry, No. 15 Shangdian Road, Cangshan District, Fuzhou, Fujian
            350002, China"
FEATURES             Location/Qualifiers
     source          1..9425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      414..778
                     /note="nptII; promoter of transference"
                     /regulatory_class="promoter"
     CDS             824..1537
                     /label="GFP"
                     /note="Aequorea victoria green fluorescent protein"
     CDS             2313..4433
                     /gene="hlyB"
                     /label="Alpha-hemolysin translocation ATP-binding protein
                     HlyB"
                     /note="Alpha-hemolysin translocation ATP-binding protein
                     HlyB from Escherichia coli. Accession#: P10089"
     CDS             4455..5888
                     /gene="hlyD"
                     /label="Hemolysin secretion protein D, chromosomal"
                     /note="Hemolysin secretion protein D, chromosomal from
                     Escherichia coli. Accession#: P09986"
     CDS             6385..7176
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(7204..7220)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7228..7244)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7252..7282)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7297..7318)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7606..8194)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8368..9225)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9226..9330)
                     /label="AmpR promoter"