LOCUS       V016378                 9425 bp    DNA     circular SYN 03-JUL-2019
DEFINITION  Exported.
ACCESSION   V016378
VERSION     V016378
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Chenyan H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2019) Fujian Agriculture and Forestry University,
            College of Forestry, No. 15 Shangdian Road, Cangshan District,
            Fuzhou, Fujian 350002, China
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2019) Fujian Agriculture and Forestry University, College of
            Forestry, No. 15 Shangdian Road, Cangshan District, Fuzhou, Fujian
            350002, China"
FEATURES             Location/Qualifiers
     source          1..9425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      414..778
                     /note="nptII; promoter of transference"
                     /regulatory_class="promoter"
     CDS             824..1537
                     /label="GFP"
                     /note="Aequorea victoria green fluorescent protein"
     CDS             2313..4433
                     /gene="hlyB"
                     /label="Alpha-hemolysin translocation ATP-binding protein
                     HlyB"
                     /note="Alpha-hemolysin translocation ATP-binding protein
                     HlyB from Escherichia coli. Accession#: P10089"
     CDS             4455..5888
                     /gene="hlyD"
                     /label="Hemolysin secretion protein D, chromosomal"
                     /note="Hemolysin secretion protein D, chromosomal from
                     Escherichia coli. Accession#: P09986"
     CDS             6385..7176
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(7204..7220)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7228..7244)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7252..7282)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7297..7318)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7606..8194)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8368..9225)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9226..9330)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctctct agagtcaggc
      421 tgttacagct cagagaaagc ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa
      481 aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
      541 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
      601 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
      661 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
      721 ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatgg
      781 aattcctgat taactttata aggaggaaaa atccatggct agcatgagta aaggagaaga
      841 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      901 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      961 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttctctta
     1021 tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa cggcatgact ttttcaagag
     1081 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
     1141 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
     1201 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
     1261 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     1321 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1381 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1441 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     1501 tgctgggatt acacatggca tggatgaact atacaaaccc gggggtgcgc cggtgccgta
     1561 tccggatccg ctggaaccgg ccggggaaaa ttctcttgca aaaaatgtat tatccggtgg
     1621 aaaaggtaat gacaagctgt acggcagtga gggagcagat ctgcttgatg gcggagaagg
     1681 gaatgatctt ctgaaaggtg gatatggtaa tgatatttat cgttatcttt caggatatgg
     1741 ccatcatatt attgacgatg atggggggaa agacgataaa ctcagtttgg ctgatattga
     1801 tttccgggat gtggccttca ggcgagaagg taatgacctc atcatgtata aagctgaagg
     1861 taatgttctt tccattggcc acaaaaatgg tattacattt aaaaactggt ttgaaaaaga
     1921 gtcaggtgat atctctaatc accagataga gcagattttt gataaagacg gcagggtaat
     1981 cacaccagat tcccttaaaa aggcacttga gtatcaacag agtaataata aggcaagtta
     2041 tgtgtatggg aatgatgcat tagcctatgg aagtcagggt aatcttaatc cattaattaa
     2101 tgaaatcagc aaaatcattt cagctgcagg taattttgat gttaaagagg aaagagctgc
     2161 agcttcttta ttgcagttgt ccggtaatgc cagtgatttt tcatatggac ggaactcaat
     2221 aactttgaca gcatcagcat aatttattaa tttaaataat agcaatctta ctgggctgtg
     2281 ccacataaga ttgctatttt tttggagtca taatggattc ttgtcataaa attgattatg
     2341 ggttatacgc cctggagatt ttagcccaat accataacgt ctctgttaac ccggaagaaa
     2401 ttaaacatag atttgacaca gacgggactg gtctgggatt aacgtcatgg ttgcttgctg
     2461 cgaaatcttt agaactaaag gtaaaacagg taaaaaaaac aattgaccga ttaaacttta
     2521 tttctctgcc cgcattagtc tggagagagg atggatgtca ttttattctg actaaagtca
     2581 gtaaagaagc aaacagatat cttatttttg atctggagca gcgaaatccc cgtgttctcg
     2641 aacagtctga gtttgaggcg ttatatcagg ggcatattat tcttattgct tcccgttctt
     2701 ctgttaccgg gaaactggca aaatttgact ttacctggtt tattcctgcc attataaaat
     2761 acaggaaaat atttattgaa acccttgttg tatctgtttt tttacaatta tttgcattaa
     2821 taacccccct tttttttcag gtggttatgg acaaagtatt agtacacagg gggttttcaa
     2881 cccttaatgt tattactgtc gcattatctg ttgtggtggt gtttgagatt atactcagcg
     2941 gtttaagaac ttacattttt gcacatagta caagtcggat tgatgttgag ttgggtgcca
     3001 aactcttccg gcatttactg gcgctaccga tctcttattt tgagagtcgt cgtgttggtg
     3061 atactgttgc cagggtaaga gaattagacc agatccgtaa tttcctgaca ggacaggcat
     3121 taacatctgt tctggactta ttattttcat tcatattttt tgcggtaatg tggtattaca
     3181 gcccaaagct tactctggtg atcttatttt cgctgccctg ttatgctgca tggtctgttt
     3241 ttattagccc cattttgcga cgtcgccttg atgataagtt ttcacggaat gcggataatc
     3301 aatctttcct ggtggaatca gtcacggcga ttaacactat aaaagctatg gcagtctcac
     3361 ctcagatgac gaacatatgg gacaaacaat tggcaggata tgttgctgca ggctttaaag
     3421 tgacagtatt agccaccatt ggtcaacaag gaatacagtt aatacaaaag actgttatga
     3481 tcatcaacct gtggttgggt gcacacctgg ttatttccgg ggatttaagt attggtcagt
     3541 taattgcttt taatatgctt gcaggtcaga ttgttgcacc ggttattcgc cttgcacaaa
     3601 tctggcagga tttccagcag gttggtatat cagttacccg ccttggtgat gtgcttaact
     3661 ctccaactga aagttatcat gggaaactgg cattaccgga aattaatggt gatatcactt
     3721 ttcgtaatat ccggtttcgc tataagcctg actctccggt tattttagat aatatcaatc
     3781 tcagtattaa gcagggggag gttattggta ttgtcggacg ttctggttca ggaaaaagca
     3841 cattaactaa attaattcaa cgtttttata ttcctgaaaa tggccaggtc ttaattgatg
     3901 gacatgatct tgcgttggcc gatcctaact ggttacgtcg tcaggtgggg gttgtgttgc
     3961 aggacaatgt gctgcttaat cgcagtatta ttgataatat ttcactggct aatcctggca
     4021 tgtccgtcga aaaagttatt tatgcagcga aattagcagg tgctcatgat tttatttctg
     4081 aattgcgtga ggggtataac accattgtcg gggaacaggg ggcaggatta tccggaggtc
     4141 aacgtcaacg catcgcaatt gcaagggcgc tggtgaacaa ccctaaaata ctcatctttg
     4201 atgaagcaac cagtgctctg gattatgagt cggagcatgt catcatgcgc aatatgcaca
     4261 aaatatgtaa gggcagaacg gttataatca ttgctcatcg tctgtctaca gtaaaaaatg
     4321 cagaccgcat tattgtcatg gaaaaaggga aaattgttga acagggtaaa cataaggagc
     4381 tgctttctga accggaaagt ttatacagtt acttatatca gttacagtca gactaacaga
     4441 aagaacagaa gaatatgaaa acatggttaa tggggttcag cgagttcctg ttgcgctata
     4501 aacttgtctg gagtgaaaca tggaaaatcc ggaagcagtt agatactccg gtacgtgaaa
     4561 aggacgaaaa tgaattctta cccgctcatc tggaattaat tgaaacgccg gtatccagac
     4621 ggccgcgtct ggttgcttat tttattatgg ggtttctggt tattgctttc attttatctg
     4681 ttttaggcca ggtggaaatt gttgccactg caaatgggaa attaacactc agtgggcgta
     4741 gcaaagaaat taaacctatt gaaaactcga tagttaaaga aattatcgta aaagaaggag
     4801 agtcagtccg gaaaggggat gtgttattaa agcttacagc gctgggagct gaagctgata
     4861 cgttaaaaac gcagtcatca ctgttacagg ccaggctgga acaaattcgg tatcaaattc
     4921 tgagccggtc aattgaatta aataaacttc ctgaactgaa acttcctgat gagccttatt
     4981 ttcagaatgt atctgaagag gaagtactgc gtttaacttc tttgataaaa gaacagtttt
     5041 ccacatggca aaatcagaag tatcaaaaag aactgaatct ggataagaaa agagcagagc
     5101 gattaacaat acttgcccgt ataaaccgtt atgaaaatgt atcgagggtt gaaaaaagcc
     5161 gtctggatga tttcaggagc ctgttgcata aacaggcaat tgcaaaacat gctgtacttg
     5221 agcaggagaa taaatatgtt gaggcagcaa atgaattacg ggtttataaa tcgcaactgg
     5281 agcaaattga gagtgagata ttgtctgcaa aagaagaata tcagcttgtc acgcagcttt
     5341 ttaaaaatga aattttagac aagctaagac aaacaacaga cagcattgag ttattaactc
     5401 tggagttaga gaaaaatgaa gagcgtcaac aggcttcagt aatcagggcc cctgtttcgg
     5461 gaaaagttca gcaactgaag gttcatactg aaggtggggt tgttacaaca gcggaaacac
     5521 tgatggtcat cgttccggaa gatgacacgc tggaggttac tgctctggta caaaataaag
     5581 atattggttt tattaacgtc gggcagaatg ccatcattaa agtggaggct tttccttaca
     5641 cccgatatgg ttatctggtg ggtaaggtaa aaaatataaa tttagatgca atagaagacc
     5701 agaaactggg actcgttttt aatgtcattg tttctgttga agagaatgat ttgtcaaccg
     5761 ggaataagca cattccatta agctcgggta tggctgtcac tgcagaaata aagactggaa
     5821 tgcgaagcgt aatcagttat cttcttagtc ccctggaaga atctgtaaca gaaagtttac
     5881 atgagcgtta agtctcagag cagcggtatc cggctcatat cttctcctgt catgtgtgac
     5941 tgcactgttt tgtggcaaac cagtactgac tatccaccgg aattcggtag tcattaacta
     6001 tggcattgat gtcctttata actgtcttac agggcgtata acacatcccg cgacggaagc
     6061 ttactagtgc atgcgaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
     6121 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     6181 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     6241 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     6301 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa
     6361 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6661 ctattgggcg aagtgccggg gcaggatctc ctgtcatccc accttgctcc tgccgagaaa
     6721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6901 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     7021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     7081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     7141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttgaaaagct tggcgtaatc
     7201 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7261 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7321 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7381 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7441 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7501 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7681 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7741 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7801 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7921 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8041 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8101 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8221 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8281 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8341 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8401 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8461 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8521 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8581 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8641 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8701 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8761 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8821 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8881 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8941 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9001 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9061 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9121 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9181 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9241 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9361 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9421 tcgtc
//
