pMDE7 vector (V016324)

LOCUS       V016324                 9703 bp    DNA     circular SYN 19-JUL-2021
DEFINITION  Exported.
ACCESSION   V016324
VERSION     V016324
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Sekurova ON, Sun YQ, Zehl M, Ruckert C, Stich A, Busche T,
            Kalinowski J, Zotchev SB.
  TITLE     Coupling of the engineered DNA 'mutator' to a biosensor as a new
            paradigm for activation of silent biosynthetic gene clusters in
            Streptomyces
  JOURNAL   Nucleic Acids Res. (2021) In press
   PUBMED   34197612
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   Sekurova ON, Sun Y-Q., Zehl M, Ruckert C, Stich A, Busche T,
            Kalinowski J, Zotchev SB.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2021) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
REFERENCE   3  (bases 1 to 9703)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: canu v. 2.1.1
            Sequencing Technology :: Illumina; ONT
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAR-2021) CeBiTec, Bielefeld University, Universitaetsstr. 27,
            Bielefeld, NRW 33615, Germany"
FEATURES             Location/Qualifiers
     source          1..9703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..40
                     /regulatory_class="promoter"
     gene            99..3638
                     /gene="dnaE1"
                     /label="dnaE1"
     CDS             99..3638
                     /codon_start=1
                     /transl_table=11
                     /gene="dnaE1"
                     /product="DnaE1"
                     /label="dnaE1"
                     /protein_id="QXN60066.1"
                     /translation="MSKPPFTHLHVHTQYSLLNGAARLKDMFDACNEMGMSHIAMSDHG
                     NLHGAYDFFHSAKKAGVTPIIGIEAYVAPESRRNKRKIQWGQPHQKRDDVSGSGGYTHK
                     TMWATNSKGLHNLFRLSSDAYAEGWLQKWPRMDKETISQWSEGIVASTGGPSGEVQTRL
                     RLGHFDEALKAAADYQDIFGKDRYFLELMDHGIEIEHRVRDGLLEIGRKLGIPPLVTND
                     SHYTYAHEATAHDALLCIQTGKNLSDPDRFRFDGTGYYLKSTDEMYAIDSSDAWQEGCA
                     NTRLIAEMIDTTGMFEKRDLMPKFDIPEGFTEITWFQEEVRRGMERRFPGGVPEDRQKQ
                     AEYEMDVIIQMGFPGYFLVVADFIMWAKNQGIAVGPGRGSAAGSIVAYAMGITDLDPIP
                     HGLIFERFLNPERVSMPDVDIDFDERRRVEVIRYVTEKYGADKVAMIGTYGKIKAKNAI
                     KDSARVLGYPYAMGDRLTKAMPADVLGKGIDLNGITDPTHPRYSEAGEIRSMYESEPDV
                     KKVIDTAKGVEGLVRQMGVHAAGVIMSSEPIVDHAPIWVRHTDGVTITQWDYPQCESLG
                     LLKMDFLGLRNLTIMDDAVKMVKSNKGIDLDLLSLPLDDPTTFELLQRGDTLGVFQFDG
                     GPMRSLLRLMKPDNFEDISAVSALYRPGPMGMDSHTNYALRKNGLQEITPIHKELEEPL
                     QEVLAVTYGLIVYQEQVQKAAQIIAGYSLGEADILRRVMGKKKPDELAKNFVLFQAGAR
                     KNGYSDEAIQALWDVLVPFAGYAFNKAHSAAYGLVSYWTGYLKANYPAEYMAALLTSVK
                     DDKDKSAVYLNECRRMGIKVLPPNVNESMSNFAAQGDDVILFGLSAVRNVGTNVVESII
                     KCRKAKGKYASFPDYLDKVEAVVCNKRTTESLIKAGAFDEMGHTRKGLTAQYEPMIDNV
                     VAVKRKEAEGQFDLFGGMGDEQSDEPGFGLDVVFGEDEWDKTYLLAQEREMLGLYVSDH
                     PLFGLEHVLSDKADAGISQLTGGDFGDGAVVTIGGIISGLQRKMTKQGNAWAIATVEDL
                     AGSLECMFFPATYQLVSTQLVEDAVVFVKGRLDKREDVPRLVAMELMIPDLSNAGTNAP
                     VVLTIPATRITPPMVSRLGEILTHHRGDSEVRIKLQGPTKTTVLRLDRHRVKPDPALFG
                     DLKVLLGPSCLAG"
     gene            complement(3758..3770)
                     /gene="lacZa'"
                     /label="lacZa'"
                     /note="lacZ alpha peptide"
     primer_bind     complement(3771..3787)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3795..3811)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3819..3849)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3864..3885)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4173..4761)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             4948..5943
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     oriT            6240..6349
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             6382..6750
                     /label="traJ"
                     /note="oriT-recognizing protein"
     rep_origin      complement(7407..8810)
                     /direction=LEFT
                     /label="minimal SCP2* replicon"
                     /note="minimal SCP2* replicon"
     primer_bind     9521..9537
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"