LOCUS       V016324                 9703 bp    DNA     circular SYN 19-JUL-2021
DEFINITION  Exported.
ACCESSION   V016324
VERSION     V016324
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Sekurova ON, Sun YQ, Zehl M, Ruckert C, Stich A, Busche T,
            Kalinowski J, Zotchev SB.
  TITLE     Coupling of the engineered DNA 'mutator' to a biosensor as a new
            paradigm for activation of silent biosynthetic gene clusters in
            Streptomyces
  JOURNAL   Nucleic Acids Res. (2021) In press
   PUBMED   34197612
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   Sekurova ON, Sun Y-Q., Zehl M, Ruckert C, Stich A, Busche T,
            Kalinowski J, Zotchev SB.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2021) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
REFERENCE   3  (bases 1 to 9703)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: canu v. 2.1.1
            Sequencing Technology :: Illumina; ONT
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAR-2021) CeBiTec, Bielefeld University, Universitaetsstr. 27,
            Bielefeld, NRW 33615, Germany"
FEATURES             Location/Qualifiers
     source          1..9703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..40
                     /regulatory_class="promoter"
     gene            99..3638
                     /gene="dnaE1"
                     /label="dnaE1"
     CDS             99..3638
                     /codon_start=1
                     /transl_table=11
                     /gene="dnaE1"
                     /product="DnaE1"
                     /label="dnaE1"
                     /protein_id="QXN60066.1"
                     /translation="MSKPPFTHLHVHTQYSLLNGAARLKDMFDACNEMGMSHIAMSDHG
                     NLHGAYDFFHSAKKAGVTPIIGIEAYVAPESRRNKRKIQWGQPHQKRDDVSGSGGYTHK
                     TMWATNSKGLHNLFRLSSDAYAEGWLQKWPRMDKETISQWSEGIVASTGGPSGEVQTRL
                     RLGHFDEALKAAADYQDIFGKDRYFLELMDHGIEIEHRVRDGLLEIGRKLGIPPLVTND
                     SHYTYAHEATAHDALLCIQTGKNLSDPDRFRFDGTGYYLKSTDEMYAIDSSDAWQEGCA
                     NTRLIAEMIDTTGMFEKRDLMPKFDIPEGFTEITWFQEEVRRGMERRFPGGVPEDRQKQ
                     AEYEMDVIIQMGFPGYFLVVADFIMWAKNQGIAVGPGRGSAAGSIVAYAMGITDLDPIP
                     HGLIFERFLNPERVSMPDVDIDFDERRRVEVIRYVTEKYGADKVAMIGTYGKIKAKNAI
                     KDSARVLGYPYAMGDRLTKAMPADVLGKGIDLNGITDPTHPRYSEAGEIRSMYESEPDV
                     KKVIDTAKGVEGLVRQMGVHAAGVIMSSEPIVDHAPIWVRHTDGVTITQWDYPQCESLG
                     LLKMDFLGLRNLTIMDDAVKMVKSNKGIDLDLLSLPLDDPTTFELLQRGDTLGVFQFDG
                     GPMRSLLRLMKPDNFEDISAVSALYRPGPMGMDSHTNYALRKNGLQEITPIHKELEEPL
                     QEVLAVTYGLIVYQEQVQKAAQIIAGYSLGEADILRRVMGKKKPDELAKNFVLFQAGAR
                     KNGYSDEAIQALWDVLVPFAGYAFNKAHSAAYGLVSYWTGYLKANYPAEYMAALLTSVK
                     DDKDKSAVYLNECRRMGIKVLPPNVNESMSNFAAQGDDVILFGLSAVRNVGTNVVESII
                     KCRKAKGKYASFPDYLDKVEAVVCNKRTTESLIKAGAFDEMGHTRKGLTAQYEPMIDNV
                     VAVKRKEAEGQFDLFGGMGDEQSDEPGFGLDVVFGEDEWDKTYLLAQEREMLGLYVSDH
                     PLFGLEHVLSDKADAGISQLTGGDFGDGAVVTIGGIISGLQRKMTKQGNAWAIATVEDL
                     AGSLECMFFPATYQLVSTQLVEDAVVFVKGRLDKREDVPRLVAMELMIPDLSNAGTNAP
                     VVLTIPATRITPPMVSRLGEILTHHRGDSEVRIKLQGPTKTTVLRLDRHRVKPDPALFG
                     DLKVLLGPSCLAG"
     gene            complement(3758..3770)
                     /gene="lacZa'"
                     /label="lacZa'"
                     /note="lacZ alpha peptide"
     primer_bind     complement(3771..3787)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3795..3811)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3819..3849)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3864..3885)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4173..4761)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             4948..5943
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     oriT            6240..6349
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             6382..6750
                     /label="traJ"
                     /note="oriT-recognizing protein"
     rep_origin      complement(7407..8810)
                     /direction=LEFT
                     /label="minimal SCP2* replicon"
                     /note="minimal SCP2* replicon"
     primer_bind     9521..9537
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 agcagggctc caaaactaac gcctgatgta ggatcagatg tgaacccttg ttcatttatg
       61 aatcatgatt caactagtcg agcaacggag gtacggacat gtcaaagccg ccgttcacgc
      121 acctgcacgt ccacacccag tactcgctgc tgaacggtgc cgcgcggctc aaggacatgt
      181 tcgacgcgtg caacgagatg ggcatgtcgc acatcgccat gtccgaccac ggcaacctgc
      241 acggggcgta cgacttcttc cactccgcga agaaggccgg agtcaccccg atcatcggga
      301 tcgaggcgta cgtcgccccc gagtcccggc gcaacaagcg caagatccag tggggccagc
      361 cgcaccagaa gcgggacgac gtctcgggtt cgggcggtta cacccacaag accatgtggg
      421 cgacgaacag caagggcctg cacaacctct tccggctctc ctccgacgcc tacgccgagg
      481 gctggctgca gaagtggccg cggatggaca aggagaccat cagccagtgg tccgagggca
      541 tcgtcgcctc caccggcggc ccctccggcg aggtgcagac ccggctgcgc ctcggccact
      601 tcgacgaggc cctgaaggcc gccgccgact accaggacat cttcggcaag gaccgttact
      661 tcctggagct gatggaccac ggcatcgaga tcgagcaccg ggtccgcgac ggactcctgg
      721 agatcggcag gaagctcggc atcccgccgc tggtgacgaa cgactcgcac tacacctacg
      781 cgcacgaggc gaccgcccac gacgccctgc tgtgcatcca gaccggcaag aacctctccg
      841 acccggaccg cttccgcttc gacggcaccg gctactacct gaagtcgacg gacgagatgt
      901 acgccatcga ctcctccgac gcctggcagg agggctgcgc caacacgcgc ctgatcgccg
      961 agatgatcga caccaccggc atgttcgaga agcgcgacct catgccgaag ttcgacatcc
     1021 ccgagggctt cacggagatc acctggttcc aggaggaggt ccgccgcggc atggagcgcc
     1081 gcttcccggg cggcgtcccc gaggaccgcc agaagcaggc cgagtacgag atggacgtca
     1141 tcatccagat ggggttcccg gggtacttcc tggtggtcgc cgacttcatc atgtgggcca
     1201 agaaccaggg catcgcggtc ggccccggcc gaggctccgc ggccggctcg atcgtggcct
     1261 acgcgatggg catcaccgac ctcgacccca tcccgcacgg cctgatcttc gagcgcttcc
     1321 tgaaccccga gcgcgtctcc atgcccgacg tcgacatcga cttcgacgag cgcaggcgcg
     1381 tcgaggtgat caggtacgtg accgagaagt acggcgccga caaggtcgcc atgatcggca
     1441 cctacggcaa gatcaaggcg aagaacgcca tcaaggactc cgcgcgcgtg ctgggctacc
     1501 cgtacgcgat gggggaccgg ctcaccaagg ccatgcccgc cgacgtcctc ggcaagggca
     1561 tcgacctcaa cggcatcacc gacccgacgc acccgcgcta cagcgaggcc ggcgagatcc
     1621 gctcgatgta cgagagcgag ccggacgtca agaaggtcat cgacaccgcc aagggcgtcg
     1681 agggcctggt ccggcagatg ggcgtgcacg cggccggcgt gatcatgtcc agcgagccca
     1741 tcgtcgacca cgccccgatc tgggtccggc acacggacgg cgtgaccatc acgcagtggg
     1801 actacccgca gtgcgagtcg ctcggcctgc tgaagatgga cttcctcggc ctgcgcaacc
     1861 tcacgatcat ggacgacgcc gtcaagatgg tgaagtccaa caagggcatc gacctcgacc
     1921 tgctgtcgct gccgctggac gaccccacga ccttcgaact gctccagcgc ggcgacacgc
     1981 tcggcgtctt ccagttcgac ggcggcccga tgcgctccct gctgcggctg atgaagcccg
     2041 acaacttcga ggacatctcc gccgtctccg cgctctaccg ccccggcccg atgggcatgg
     2101 actcgcacac caactacgcg ctgcgcaaga acggcctcca ggagatcacg ccgatccaca
     2161 aggagctgga ggagcccctc caggaggtcc tcgcggtcac ctacggcctg atcgtctatc
     2221 aggagcaggt gcagaaggcc gcccagatca tcgcgggcta ctcgctcggc gaggccgaca
     2281 tcctgcgccg cgtgatgggc aagaagaagc ccgacgaact ggcgaagaac ttcgtcctct
     2341 tccaggccgg agccaggaag aacggctaca gcgacgaggc catccaggcc ctgtgggacg
     2401 tcctggtccc gttcgccggc tacgccttca acaaggcgca ctcggccgcc tacggcctgg
     2461 tctcgtactg gaccggatac ctgaaggcga actacccggc cgagtacatg gccgccctgc
     2521 tcacctcggt caaggacgac aaggacaagt cggccgtcta cctgaacgag tgccggcgca
     2581 tgggcatcaa ggtgctgccg cccaacgtca acgagtccat gtcgaacttc gccgcgcagg
     2641 gcgacgacgt gatcctcttc ggcctctccg ccgtgcgcaa cgtcggcacc aacgtcgtcg
     2701 agtcgatcat caagtgccgc aaggccaagg ggaagtacgc gtccttcccc gactacctgg
     2761 acaaggtgga ggcggtcgtc tgcaacaagc ggacgacgga gtcgctgatc aaggcgggcg
     2821 ccttcgacga gatggggcac acccgcaagg gcctcaccgc gcagtacgaa ccgatgatcg
     2881 acaacgtggt cgcggtcaag cgcaaggagg ccgagggcca gttcgacctc ttcggcggca
     2941 tgggcgacga gcagagcgac gagcccggct tcggactcga cgtggtcttc ggcgaggacg
     3001 agtgggacaa gacctatctg ctcgcccagg agcgggagat gctcggtctc tacgtctccg
     3061 accacccgct cttcggcctg gagcacgtgc tgtccgacaa ggccgacgcg ggcatctccc
     3121 agctcaccgg cggtgacttc ggggacggcg cggtcgtcac catcggcggc atcatctcgg
     3181 gcctccagcg caagatgacc aagcagggca acgcctgggc catcgccacc gtcgaggacc
     3241 tggccggctc gctggagtgc atgttcttcc cggcgacgta ccagctggtg tccacgcaac
     3301 tggtcgagga cgccgtggtg ttcgtcaagg ggcgcctcga caagcgggag gacgtgcccc
     3361 ggctggtcgc gatggagctg atgattcccg acctgtccaa cgcgggcacc aacgcccccg
     3421 tcgtcctcac catcccggcg acccgcatca cgccgccgat ggtcagcagg ctcggtgaga
     3481 tcctcaccca ccaccggggc gacagcgagg tgcggatcaa gctccagggg cccacgaaga
     3541 cgacggtgct gcgcctggac cggcaccggg tgaagcccga tccggcgctg ttcggcgacc
     3601 tgaaggtgct gctcgggccg tcctgcctgg ccggctgagc ggcatcccgg accacgacga
     3661 gcgagggcgc acccggttgc cgggtgcgcc cttcgtcgtg gctgtcagtt gtggccgaag
     3721 cgcttgttcc gcttgcgggc ggccgcgcgc gatatcgaat tcgtaatcat gtcatagctg
     3781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3901 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3961 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4021 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4081 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4681 tccggcaaac aaaccaccga tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4741 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4801 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4861 tagatccttt tggttcatgt gcagctccat ctcgcgaccg gccgtgcgga attaagccgg
     4921 cccgtaccct gtgaatagag gtccgctgtg acacaagaat ccctgttact tctcgaccgt
     4981 attgattcgg atgattccta cgcgagcctg cggaacgacc aggagttctg ggagccgctg
     5041 gcccgccgag ccctggagga gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc
     5101 gagagcacca accccgtact ggtcggcgag cccggcccgg tgatcaagct gttcggcgag
     5161 cactggtgcg gtccggagag cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac
     5221 gccccggtgc cggtgccccg cctcctcggc cgcggcgagc tgcggcccgg caccggagcc
     5281 tggccgtggc cctacctggt gatgagccgg atgaccggca ccacctggcg gtccgcgatg
     5341 gacggcacga ccgaccggaa cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc
     5401 ggccggctgc acagggtgcc gctgaccggg aacaccgtgc tcacccccca ttccgaggtc
     5461 ttcccggaac tgctgcggga acgccgcgcg gcgaccgtcg aggaccaccg cgggtggggc
     5521 tacctctcgc cccggctgct ggaccgcctg gaggactggc tgccggacgt ggacacgctg
     5581 ctggccggcc gcgaaccccg gttcgtccac ggcgacctgc acgggaccaa catcttcgtg
     5641 gacctggccg cgaccgaggt caccgggatc gtcgacttca ccgacgtcta tgcgggagac
     5701 tcccgctaca gcctggtgca actgcatctc aacgccttcc ggggcgaccg cgagatcctg
     5761 gccgcgctgc tcgacggggc gcagtggaag cggaccgagg acttcgcccg cgaactgctc
     5821 gccttcacct tcctgcacga cttcgaggtg ttcgaggaga ccccgctgga tctctccggc
     5881 ttcaccgatc cggaggaact ggcgcagttc ctctgggggc cgccggacac cgcccccggc
     5941 gcctgacgcc ccctctagct ggcgggaacg gcaatgcttg gacggctgat ccgattggca
     6001 cggcggacgg cgaatggcgg agcagacgct cgtccggggg caatgagata tgaaaaagcc
     6061 tgaactcacc gcgacgtatc gatctagtat gcaggtcgac ggatcttttc cgctgcataa
     6121 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     6181 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     6241 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     6301 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc
     6361 gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca
     6421 cctatcaagg tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg
     6481 gccggcatga gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc
     6541 gtcgtggact atgagcacgt ccgcgagctg gcccgcatca atggcgacct gggccgcctg
     6601 ggcggcctgc tgaaactctg gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc
     6661 acgatcctcg ccctgctggc gaagatcgaa gagaagcagg acgagcttgg caaggtcatg
     6721 atgggcgtgg tccgcccgag ggcagagcca tgactttttt agccgctaaa acggccgggg
     6781 ggtgcgcgtg attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga
     6841 gctggtgaag tacatcaccg acgagcaagg caagaccgat ccccggggac ctgcatagat
     6901 cccgccgccg cgcgtctcgc cggacctggc tctaacgcct gtggccccca cgattccggg
     6961 gtgcgtccgg ctggagcgca ccctgaccgc tcggatgctc gcctggttcg agtgcgaaga
     7021 cttcggcctt ccgccctccg cgagcaagcc tgtgatgctg taccggaccg tccagtacga
     7081 cgcgaagggc gacgtcctgg ccgtgacgca gacgctcgtt ggcgatggac acgagcagcc
     7141 tgaaggcttc gagcttcctc ccgtacaggg cggcaactga ccgtctggca catagatcag
     7201 cgcccccgct ccgtgggaga tcggagcggg ggcgctgtgt tgtgcggtca agagacgggg
     7261 cggatcgtcc ggcggcggcg agcgtcggtg ttgctgacct tgcggggcgc gcgggcacgg
     7321 tcgtccgggg tgaccggggg gtccgtgagg gcggcggtga tcatctcgcg cagctcgggg
     7381 tgcagcgtga agtagtaccg ggcgttgatc cggtagaggc cggcggaggt ccgcacgagg
     7441 aggttcaggg cctccaggcg ggcgagagcc tcgtgcaccg tggagacgga gttgccgcag
     7501 tcctcggcga cggtttcgac cggtacgggg atgacgtcaa acaggccgcc gtggtcctcc
     7561 acccacgcga gcgtgcagtg ctgagcggcg gacaggttca gcgagtacag cgacggggcg
     7621 gtcaggtcgc gctccgggtt gaagccttct acgggcatgg tcgtcatcgc gtctctcctg
     7681 cggcgcggcg gcgggtagcc gggatggtct tgggcttgct ggcggacctg gtcatggcct
     7741 tgtcgtccgg ggccacacgc ggcggcgcca tgcggcggca ggcgttgacc tgcgtgctcc
     7801 cggctccctc ccagtatcgg agggggttca gctggtaaga gccggatcgc gggcccggct
     7861 tgaccacaag cccgagcttg accagccggt taagggcctt ccggcaggtg tccttgctgg
     7921 cgccgacgtc ctcgcccagc tcctcgggcg tgaccgggac gtatccggcc gcgcctccgt
     7981 gttcgcggag ccattcgagc gtcgcccact ccgctgcggc cagcccgagg gagtacaggg
     8041 ggacgtcgcg cctacggcct gcaaggtgta ccccttcccg tcgaactggt gcggagtccg
     8101 cggcctggcg gacaccgcct ctccggtgtc catgttgacc agggccatgt tcggcccgcg
     8161 cttcctcgtc ggcactcgcg cctctcccat cctcgcgcta aggggcggtt tttgacccct
     8221 tagctccgat gaaccgccgc ttacgagagg aaactaacag gtcaccactc cgaaaaacag
     8281 ccgcttagtc cgcgtgtcac acgcataggt gacgcgactt cctggcgacc gggcgcgacg
     8341 agggacgcac ggtgggtagc taagcggcga tttttgaccc agtaaccggc ggtttttagc
     8401 cgcttagccg cgcgttttac caggtcaaga gcatgcgccc cttcttcact agaaggcgcg
     8461 cgccgtcact cacgcaccac ggcgcacacc gtggtgaccg ccctggactc ctacccgccc
     8521 ggcataccgg gccgctctta gcttctacgg ggaaggccgg cggtcctgcg cacctggtcc
     8581 tcgtcggcgc ctacggtcgg cgagtcctca gccctggcgg gcttgcgggt cccgactgat
     8641 gccacctgat cacgcctggc gaccgcacgc tgtcctcggc cggccacaag caggaaggaa
     8701 gcgtcgtcct gccggtgggc ggctgaggta cctccgtacc gccgccgttg tcgcccccgc
     8761 cgcaggggtt gcctttctgt ggctgaggtc gattcccccg ccattgatca cccgcaccag
     8821 ggagggccca gccgacgagg tggcgcgcgt gcgcacgtcg ctgggtgggg gtgcggtagg
     8881 taccgggcag cagctcgggg accaggcccc ggcgggcggg ggtccggagg agcccgccgg
     8941 gggtgtgcgc aagggcgtag cccgtagccc cggagcgcag cggaggggtc gctagtagga
     9001 ggccgctatg gcgcgtacca ccagcccaca gggtccggcc tccggaggcg acgcactgcc
     9061 ggaccggacc gcaaggtccg gtgagcccac aacggcggat ctacgtctgt cgagaagttt
     9121 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     9181 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     9241 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     9301 ccggaagtgc ttgacattgg ggaatttatg cggtgtgaaa taccgcacag atgcgtaagg
     9361 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     9421 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     9481 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     9541 aagcttgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9601 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9661 cattaaccta taaaaatagg cgtatcacga ggccctttcg tct
//
