GST-SMXL8 vector (V016317)

MSDS

Basic Vector Information

Vector Name:: GST-SMXL8

Antibiotic Resistance:: Ampicillin

Length:: 7713 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Wang L.

GST-SMXL8 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GST-SMXL8 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_1091        7713 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector GST-SMXL8, complete sequence.
ACCESSION   MN728554
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (23-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             918..941
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             966..3695
                     /gene="SMXL8"
                     /label=SMXL8
                     /note="Protein SMAX1-LIKE 8 from Arabidopsis thaliana. 
                     Accession#: F4IGZ2"
     promoter        4016..4120
                     /label=AmpR promoter
     CDS             4121..4978
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5152..5740
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5984..6061
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6062..7141
                     /label=lacI
                     /note="lac repressor"
     protein_bind    7157..7178
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7193..7223
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             7267..7440
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"

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