GST-SMXL8 vector (V016317)

LOCUS       V016317                 7713 bp    DNA     circular SYN 20-JAN-2020
DEFINITION  Exported.
ACCESSION   V016317
VERSION     V016317
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2019) State Key Laboratory of Plant Genomics,
            Institute of Genetics and Developmental Biology, CAS, West Beichen
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (23-NOV-2019) State Key Laboratory of Plant Genomics, Institute of
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             918..941
                     /label="HRV 3C site"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             966..3695
                     /gene="SMXL8"
                     /label="Protein SMAX1-LIKE 8"
                     /note="Protein SMAX1-LIKE 8 from Arabidopsis thaliana.
                     Accession#: F4IGZ2"
     promoter        4016..4120
                     /label="AmpR promoter"
     CDS             4121..4978
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5152..5740
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        5984..6061
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6062..7141
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    7157..7178
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7193..7223
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             7267..7440
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"