LOCUS       V016317                 7713 bp    DNA     circular SYN 20-JAN-2020
DEFINITION  Exported.
ACCESSION   V016317
VERSION     V016317
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2019) State Key Laboratory of Plant Genomics,
            Institute of Genetics and Developmental Biology, CAS, West Beichen
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (23-NOV-2019) State Key Laboratory of Plant Genomics, Institute of
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             918..941
                     /label="HRV 3C site"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             966..3695
                     /gene="SMXL8"
                     /label="Protein SMAX1-LIKE 8"
                     /note="Protein SMAX1-LIKE 8 from Arabidopsis thaliana.
                     Accession#: F4IGZ2"
     promoter        4016..4120
                     /label="AmpR promoter"
     CDS             4121..4978
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5152..5740
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        5984..6061
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6062..7141
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    7157..7178
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7193..7223
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             7267..7440
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc ccggaattcc
      961 cggggatgcc aacggcggtg aatgtagcga aacaatgctt aacggcagaa gcatcctacg
     1021 cattagaaga agctgtgaac gttgcacgtc gaagaggaca ctcacaaacg acgtcgcttc
     1081 acgctatctc agctttactc tctctaccaa cctcagtctt acgtgacgca tgtgctcgtg
     1141 ttcgtaactc tgcttactct cctcgtcttc aattcaaagc tttagatctt tgcttaagcg
     1201 tttctttaga ccggatccag tcgggtcatc aacttggatc tgatgattca ccacctgttt
     1261 ctaactctct aatggctgca attaaacgat ctcaagcgca tcaacgtcgt ttacctgaga
     1321 acttcaggat ctatcaagaa atgtcacaga gtcagaatca gaactcactt tcttgtgtga
     1381 aagtggagct tcgtcagttg attctttcga tcttggatga tccggttgtg agccgggttt
     1441 ttggtgaagc gggttttcgg agctccgagt taaagctttc cattattcga cctgttcctc
     1501 atcttcttcg ttattcatca caacagcctc tgtttctctg taacctaacc gggaatcctg
     1561 aaccgaatcc ggttcgatgg ggtttcaccg taccaagcct gaattttaac ggagatttgg
     1621 attaccggag gattagcgcg gttttcacga aagataaagg gaggaatcct ctgcttgttg
     1681 gtgtatcagc ttacggtgta ctaaccagtt atttaaattc gctagagaaa aaccaaaccg
     1741 acgggatgat tctcccgacg aagcttcacg gtttaacggc ggttaacatc ggttcagaaa
     1801 tctccgacca aatcagcgtc aaattcgata aaacctacac cgacactagg tttcatgatc
     1861 tagggaagct tgcagagcaa ggttcaggac ctggactact cttacactac ggtgatctta
     1921 gggttttcac caacggcgaa ggaaacgtac cggcggcgaa ttacatcgtg aatcggattt
     1981 cggagctgtt gcggcgacac gggagaagag tgtggttgat cggagcgacg acgagcaacg
     2041 aggtttatga gaagatgatg agaaggtttc cgaatgtgga gaaagattgg gatttgcagt
     2101 tactcaccat cacttctctt aagccttgtt tgcctcacaa caagtccagt ttgataggat
     2161 catttgttcc gtttggtgga ttcttctcaa caactccttc tgaactgaaa ctacccttct
     2221 ccggtttcaa aacggaaatc accggacccg tttcttcaat atccgatcag acacaatcta
     2281 ccttgccacc ttggttacaa atgaccacaa gaactgattt aaaccaaaaa tcaagtgcca
     2341 aggtcgtgca gaccaaagaa gggttggaat cagtttgtgg aaataagttc acgagcagtg
     2401 cttctgcatc gacctgttca gctaaatcgg tcaccactga tctgaatctt agggtaagtt
     2461 cagtcaccac aggatccggt ctcaagaagc atttggatag caaagatttt tctcagccac
     2521 aatctgtgtc ctcctattcc tttgataacc caagggacct caacgcagag agtttcaaga
     2581 ttatttacag aagattaacc gatatggttt caggacaaga tgaggctgct agagttatca
     2641 gctgtgcact gtcacaaccg cctaagagtg tcactcgaag agatgtttgg cttaatttgg
     2701 ttggtcctga tactgtagga aagagaagaa tgtcgcttgt ccttgctgag attgtgtatc
     2761 aaagcgaaca cagatttatg gccgttgatc ttggtgccgc ggagcaggga atgggcggtt
     2821 gtgatgatcc gatgaggttg agaggaaaga cgatggttga tcatatcttt gaagtgatgt
     2881 gtaggaatcc tttctgcgtg gttttccttg agaacattga aaaagctgac gagaagctgc
     2941 agatgagctt gtcaaaggca atcgaaactg ggaagtttat ggattcacac gggagagaag
     3001 ttggtatcgg aaacactatt tttgttatga cgtcatcttc acaaggttcc gcaacaacaa
     3061 catcttattc tgaagagaaa ctattgagag tcaaaggaag gcaagtggag ataaggattg
     3121 aaactgtatc tagtttacca atggtgcgat cggtttacgg cccaacctcg gtgaacaaga
     3181 ggaagctgat gggattaggg aatcttcagg agacaaaaga tactgtggag agcgtaaaac
     3241 ggttaaacag aacaactaat ggagttcttg atctgaacct tccggctcaa gaaaccgaaa
     3301 tcgaggaaaa atatcattgt gaagagaact caaacgtttg gttaatgaac ttgaagaatc
     3361 ataaacgtct cattgaggtt cctttcaagc cttttgattt tgaaggacta gctgaaaaaa
     3421 tcaaaaagag cgttaaagaa aatttcgaca agtgcgtgag atcggattgc ttgctggagg
     3481 ttgaccctaa gatcatagaa cgattgctag cggctgttta tttctcagat agtagaaaag
     3541 atatcaaaga gttgctggag aatataatga gtccagtgtt cttgagaatt aaagaaaggt
     3601 atgaaatcac tactagttgt gtagtaaaat tagttggtcg agatcttgac atttttcttg
     3661 aggatcaaat ggacttgttc tttgtaaaat ctcagtagtc gagcggccgc atcgtgactg
     3721 actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     3781 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     3841 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     3901 cggagtgtat aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3961 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4021 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4081 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4141 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4201 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4261 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4321 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     4381 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4441 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4501 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4561 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4621 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     4681 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4741 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4801 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4861 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4921 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4981 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5041 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5101 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5161 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5221 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5281 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5341 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5401 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5461 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5521 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5581 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5641 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5701 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5761 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5821 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5881 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     5941 tctccttacg catctgtgcg gtatttcaca ccgcataaat tccgacacca tcgaatggtg
     6001 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
     6061 tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
     6121 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     6181 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
     6241 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
     6301 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
     6361 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
     6421 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
     6481 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
     6541 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
     6601 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     6661 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     6721 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
     6781 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     6841 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
     6901 atgttatatc ccgccgtcaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     6961 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     7021 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     7081 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7141 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7201 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7261 acagctatga ccatgattac ggattcactg gccgtcgttt tacaacgtcg tgactgggaa
     7321 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7381 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7441 tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat
     7501 cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg
     7561 cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt tcccacggag
     7621 aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc
     7681 cagacgcgaa ttatttttga tggcgttgga att
//
