MERSCoVS vector (V016309)

LOCUS       V016309                 8889 bp    DNA     circular SYN 21-MAR-2021
DEFINITION  Exported.
ACCESSION   V016309
VERSION     V016309
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Park SB.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) NIDDK, NIH, 10 Center Drive, Bethesda, MD
            20814, USA
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (01-FEB-2021) NIDDK, NIH, 10 Center Drive, Bethesda, MD 20814, USA"
FEATURES             Location/Qualifiers
     source          1..8889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(968..1825)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1826..1930)
                     /label="AmpR promoter"
     rep_origin      complement(1956..2411)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2553..2569
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2579..2597
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        2718..3097
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3098..3301
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          3413..3985
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             4058..8116
                     /gene="S"
                     /label="Spike glycoprotein"
                     /note="Spike glycoprotein from Middle East respiratory
                     syndrome-related coronavirus (isolate United
                     Kingdom/H123990006/2012). Accession#: K9N5Q8"
     polyA_signal    8280..8335
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(8693..8709)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(8717..8733)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8741..8771)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8786..8807)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."