Basic Vector Information
- Vector Name:
- MERSCoVS
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8889 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Park SB.
MERSCoVS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
MERSCoVS vector Sequence
LOCUS 62056_1600 8889 bp DNA circular SYN 21-MAR-2021
DEFINITION Expression vector MERSCoVS, complete sequence.
ACCESSION MW560956
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8889)
AUTHORS Park SB.
TITLE Direct Submission
JOURNAL Submitted (01-FEB-2021) NIDDK, NIH, 10 Center Drive, Bethesda, MD
20814, USA
REFERENCE 2 (bases 1 to 8889)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(01-FEB-2021) NIDDK, NIH, 10 Center Drive, Bethesda, MD 20814, USA"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..8889
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(206..794)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(968..1825)
/label=AmpR
/note="beta-lactamase"
promoter complement(1826..1930)
/label=AmpR promoter
rep_origin complement(1956..2411)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 2553..2569
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2579..2597
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
enhancer 2718..3097
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3098..3301
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 3413..3985
/label=beta-globin intron
/note="intron from rabbit beta-globin gene"
CDS 4058..8116
/gene="S"
/label=S
/note="Spike glycoprotein from Middle East respiratory
syndrome-related coronavirus (isolate United
Kingdom/H123990006/2012). Accession#: K9N5Q8"
polyA_signal 8280..8335
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(8693..8709)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(8717..8733)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8741..8771)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(8786..8807)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
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