LOCUS       V016309                 8889 bp    DNA     circular SYN 21-MAR-2021
DEFINITION  Exported.
ACCESSION   V016309
VERSION     V016309
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Park SB.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) NIDDK, NIH, 10 Center Drive, Bethesda, MD
            20814, USA
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (01-FEB-2021) NIDDK, NIH, 10 Center Drive, Bethesda, MD 20814, USA"
FEATURES             Location/Qualifiers
     source          1..8889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(206..794)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(968..1825)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1826..1930)
                     /label="AmpR promoter"
     rep_origin      complement(1956..2411)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2553..2569
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2579..2597
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        2718..3097
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3098..3301
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          3413..3985
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             4058..8116
                     /gene="S"
                     /label="Spike glycoprotein"
                     /note="Spike glycoprotein from Middle East respiratory
                     syndrome-related coronavirus (isolate United
                     Kingdom/H123990006/2012). Accession#: K9N5Q8"
     polyA_signal    8280..8335
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(8693..8709)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(8717..8733)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8741..8771)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8786..8807)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
       61 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1921 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     1981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     2041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     2101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     2161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     2221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     2281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     2341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     2401 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2461 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2521 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     2581 atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagagagct
     2641 tggcccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc
     2701 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     2761 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2821 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2881 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2941 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     3001 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     3061 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     3121 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     3181 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     3241 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     3301 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     3361 aagacaccgg gaccgatcca gcctccggtc gaccgatcct gagaacttca gggtgagttt
     3421 ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg
     3481 caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc
     3541 atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt
     3601 cttttcattt tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa
     3661 attcactttt gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg
     3721 caatcagggt atattatatt gtacttcagc acagttttag agaacaattg ttataattaa
     3781 atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg
     3841 tagaaacaac tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac
     3901 actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt
     3961 tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca
     4021 tcattttggc aaagaattcc tcgacggatc cgccaccatg attcactctg tgttcctgct
     4081 gatgttcctg ctgacaccaa cagagtccta tgtggatgtg ggacctgact ctgtgaagtc
     4141 tgcctgtatt gaggtggaca tccaacagac cttctttgac aagacctggc caagaccaat
     4201 tgatgtgagc aaggctgatg gcatcatcta cccacagggc aggacctaca gcaacatcac
     4261 catcacctac cagggactgt ttccatacca gggagatcat ggagatatgt atgtctactc
     4321 tgctggtcat gccacaggca ccacaccaca gaaactgttt gtggctaact acagccagga
     4381 tgtgaagcag tttgccaatg gctttgtggt gaggattgga gcagcagcca acagcacagg
     4441 cacagtgatt atcagcccaa gcacctctgc caccatcagg aagatttacc ctgcctttat
     4501 gctgggctcc tctgtgggca acttctctga tggcaagatg ggcaggttct tcaaccacac
     4561 cctggtgctg ctgcctgatg gctgtggcac cctgctgagg gctttctact gtatcttgga
     4621 accaaggtct ggcaaccact gtcctgctgg caactcctac acctcctttg ccacctacca
     4681 cacacctgcc acagactgtt ctgatggcaa ctacaacagg aatgcctccc tgaactcctt
     4741 caaggaatac ttcaacctga ggaactgtac ctttatgtac acctacaaca tcacagagga
     4801 tgagattttg gagtggtttg gcatcaccca gacagcccag ggagtgcatc tgttctcgag
     4861 cagatatgtg gacctctatg gaggcaatat gttccagttt gccaccctgc ctgtctatga
     4921 caccatcaaa tactacagca tcatcccaca cagcatcagg agcatccagt ctgacaggaa
     4981 ggcttgggct gccttctatg tctacaaact ccaaccactg accttcctgc tggacttctc
     5041 tgtggatggc tacatcagga gggctattga ctgtggcttc aatgacctga gccaacttca
     5101 ctgttcctat gagtcctttg atgtggagtc tggagtctac tctgtgtcct cctttgaggc
     5161 taagccatct ggctctgtgg tggaacaggc tgagggagtg gagtgtgact tcagcccact
     5221 gctgtctggc acacctccac aggtctacaa cttcaagaga ctggtgttca ccaactgtaa
     5281 ctacaacctg accaaactgc tgtccctgtt ctctgtgaat gacttcactt gtagccagat
     5341 tagccctgct gccattgcca gcaactgtta ctcctccctg attctggact acttctccta
     5401 cccactgagt atgaagtctg acctgtctgt gtcctctgct ggaccaatca gccagttcaa
     5461 ctacaagcag tccttcagca acccaacttg tctgattctg gctacagtgc cacacaacct
     5521 gaccaccatc accaagccac tgaaatactc ctacatcaac aagtgtagca gactgctgtc
     5581 tgatgacagg acagaggtgc cacaactagt gaatgccaac caatacagcc catgtgtgag
     5641 cattgtgcca agcacagtgt gggaggatgg agactactac aggaagcaac ttagcccatt
     5701 ggagggagga ggctggctgg tggcatctgg cagcacagtg gctatgacag aacaactcca
     5761 aatgggcttt ggcatcacag tccaatatgg cacagacacc aactctgtgt gtccaaaatt
     5821 ggagtttgcc aatgacacca agattgccag ccaacttggc aactgtgtgg aatactccct
     5881 ctatggagtg tctggcaggg gagtgttcca gaactgtact gctgtgggag tgagacaaca
     5941 gaggtttgtc tatgatgcct accagaacct ggtgggctac tactctgatg atggcaacta
     6001 ctactgtctg agggcttgtg tgtctgtgcc tgtgtctgtg atttatgaca aggagaccaa
     6061 gacccatgcc accctgtttg gctctgtggc ttgtgaacac atctccagca caatgagtca
     6121 atacagcagg agcaccagga gtatgctgaa aaggagggac agcacatatg gaccactcca
     6181 aacacctgtg ggctgtgtgc tgggactggt gaactcctcc ctgtttgtgg aggactgtaa
     6241 actgccactg ggacaatccc tgtgtgccct gcctgacaca ccaagcaccc tgacaccaag
     6301 gtctgtgagg tctgtgcctg gagagatgag actggcaagc attgccttca accacccaat
     6361 ccaggtggac caacttaact cctcctactt caaactgagc atcccaacca acttctcctt
     6421 tggagtgacc caggaataca tccagaccac catccagaag gtgacagtgg actgtaagca
     6481 atatgtgtgt aatggcttcc agaagtgtga acaacttctg agggaatatg gacaattctg
     6541 tagcaagata aaccaggctc ttcatggagc caacctgaga caggatgact ctgtgaggaa
     6601 cctgtttgcc tctgtgaagt ccagccagtc cagcccaatc atccctggct ttggaggaga
     6661 cttcaacctg accctgttgg aaccggtgag catcagcaca ggcagcaggt ctgccaggtc
     6721 tgccattgag gacctgctgt ttgacaaggt gaccattgct gaccctggct atatgcaggg
     6781 ctatgatgac tgtatgcaac agggacctgc ctctgccagg gacctgattt gtgcccaata
     6841 tgtggctggc tacaaggtgc tgcctccact gatggatgtg aatatggagg ctgcctacac
     6901 ctcctccctg ctgggcagca ttgctggagt gggctggact gcaggactgt cctcctttgc
     6961 tgccatccca tttgcccaga gcatcttcta cagactgaat ggagtgggca tcacccaaca
     7021 ggtgctgtct gagaaccaga aactgattgc caacaagttc aaccaggctc tgggagctat
     7081 gcagacaggc ttcaccacca ccaatgaggc tttccagaag gtccaggatg ctgtgaacaa
     7141 caatgcccag gctctgagca aactggcatc tgaactgagc aacacctttg gagccatctc
     7201 tgctagcatt ggagacatca tccagagact ggatgtgttg gaacaggatg cccagattga
     7261 cagactgata aatggcagac tgaccaccct gaatgccttt gtggctcaac aacttgtgag
     7321 gtctgagtct gctgccctgt ctgcccaact tgccaaggac aaggtgaatg agtgtgtgaa
     7381 ggctcaaagc aagaggtctg gcttctgtgg acaaggcacc cacattgtgt cctttgtggt
     7441 gaatgcccca aatggactct actttatgca tgtgggctac tacccaagca accacattga
     7501 ggtggtgtct gcctatggac tgtgtgatgc tgccaaccca accaactgta ttgcccctgt
     7561 gaatggctac ttcatcaaga ccaacaacac caggattgtg gatgagtggt cctacacagg
     7621 ctcctccttc tatgcccctg aaccaatcac ctccctgaac accaaatatg tggctccaca
     7681 ggtgacctac cagaacatca gcaccaacct gcctcctcca ctgctgggca acagcacagg
     7741 cattgacttc caggatgaac tggatgagtt cttcaagaat gtgagcacca gcatcccaaa
     7801 ctttggctcc ctgacccaga taaacaccac cctgctggac ctgacctatg agatgctgtc
     7861 cctccaacag gtggtgaagg ctctgaatga gtcctacatt gacctgaaag aactgggcaa
     7921 ctacacctac tacaacaagt ggccatggta catctggctg ggcttcatcg ctggcctggt
     7981 ggccctggcg ctgtgcgtgt tcttcatcct gtgctgcacc ggctgcggca ccaactgcat
     8041 gggcaagctg aagtgcaaca ggtgctgcga caggtacgag gagtacgacc tggagcccca
     8101 caaggtgcac gtacattaag gatccgtcga ggaattcact cctcaggtgc aggctgccta
     8161 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
     8221 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     8281 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     8341 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     8401 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
     8461 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     8521 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     8581 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     8641 ccctcttctc ttatggagat ccctcgacgg atcggccgca attcgtaatc atgtcatagc
     8701 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8761 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8821 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8881 gcgcgggga
//
