pTRV-RNA2 vector (V016281)

LOCUS       62056_21570        8928 bp DNA     circular SYN 23-NOV-2021
DEFINITION  VIGS vector pTRV-RNA2, complete sequence.
ACCESSION   MH625696
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Rahman J, Baldwin IT, Gase K.
  TITLE     California TRV-based VIGS vectors mediate gene silencing at elevated
            temperatures but with greater growth stunting
  JOURNAL   BMC Plant Biol 21, 553 (2021)
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Rahman J, Gase K, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2018) Molecular Ecology, Max-Planck-Institute for 
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, 
            Germany
REFERENCE   3  (bases 1 to 8928)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol 21, 553 (2021)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JUL-2018) Molecular Ecology, Max-Planck-Institute for Chemical 
            Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        201..546
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    568..1728
                     /note="Tobacco rattle virus California isolate segment
                     RNA2"
     CDS             1090..1692
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /label=coat protein
                     /protein_id="QDJ00478.1"
                     /translation="MTDGMYDEEFDSKALNETFSPWVEEKNWKDVLMRLSAMKFALQAD
                     RDKIPGVLSDLRKDCPFSAFKRFPDKEMWSKLTKEAVIALAQIQAASSFKRRADEKNAV
                     SGLITATPAQASTSNANPSGSATTVVRPPRLDDSSFQEDSFSFGKFDDASTAYHKALSY
                     LEGLNLKPLYRRQFEKSYNTRWVPAAAPVAPGPRPNP"
     misc_feature    1729..1759
                     /label=polylinker
                     /note="polylinker"
     misc_feature    1760..2327
                     /note="Tobacco rattle virus California isolate segment
                     RNA2"
     polyA_signal    2337..2511
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(2594..2618)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(2871..3662)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             5072..5698
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6130..7200
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7269..7463
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      complement(7906..8494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8851..8875)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"