LOCUS       62056_21570        8928 bp DNA     circular SYN 23-NOV-2021
DEFINITION  VIGS vector pTRV-RNA2, complete sequence.
ACCESSION   MH625696
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Rahman J, Baldwin IT, Gase K.
  TITLE     California TRV-based VIGS vectors mediate gene silencing at elevated
            temperatures but with greater growth stunting
  JOURNAL   BMC Plant Biol 21, 553 (2021)
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Rahman J, Gase K, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2018) Molecular Ecology, Max-Planck-Institute for 
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, 
            Germany
REFERENCE   3  (bases 1 to 8928)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol 21, 553 (2021)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JUL-2018) Molecular Ecology, Max-Planck-Institute for Chemical 
            Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        201..546
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    568..1728
                     /note="Tobacco rattle virus California isolate segment
                     RNA2"
     CDS             1090..1692
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /label=coat protein
                     /protein_id="QDJ00478.1"
                     /translation="MTDGMYDEEFDSKALNETFSPWVEEKNWKDVLMRLSAMKFALQAD
                     RDKIPGVLSDLRKDCPFSAFKRFPDKEMWSKLTKEAVIALAQIQAASSFKRRADEKNAV
                     SGLITATPAQASTSNANPSGSATTVVRPPRLDDSSFQEDSFSFGKFDDASTAYHKALSY
                     LEGLNLKPLYRRQFEKSYNTRWVPAAAPVAPGPRPNP"
     misc_feature    1729..1759
                     /label=polylinker
                     /note="polylinker"
     misc_feature    1760..2327
                     /note="Tobacco rattle virus California isolate segment
                     RNA2"
     polyA_signal    2337..2511
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(2594..2618)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(2871..3662)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             5072..5698
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6130..7200
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7269..7463
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      complement(7906..8494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8851..8875)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagctccacg tgagcttcat ggagtcaaag attcaaatag aggacctaac agaactcgcc
       61 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      121 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      181 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      241 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      301 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      361 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      421 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      481 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      541 agaacacggg ggactcttga cctgcagata aaacattgca cctatggtgt tgccccggcc
      601 ggggtgtgtc tttgaacgca gtagaatgtg ctaattgaca agttggagaa cgcggtagaa
      661 cgtacttatc cgacacggcc ttttatccct ttgttgagag gtttttctca gccgcaatga
      721 aattctggat ttagttggcc gctagaaggc tgaaggaatg tagttgtgtt tttagtttag
      781 ttaataagtg tcttccaata cacattaaac cttggacgtg ttagctacga aagtgtgtaa
      841 atttaaaaac aattagtctt ccgtgattaa ggatgattaa ggctaaaaac gattgttaat
      901 gattttggca atcgcatcca ttcgctatca caagcgatgg tggcattgac ccggaaaggt
      961 ctaatcttgc ggtcattcga ctggaagaag cataattata ctgattgtgc aactttgtgt
     1021 tactattctg acagtgattc ttgctgaact actggttact gtatcacttg caatcactca
     1081 cgtttcgcga tgacagacgg tatgtacgat gaggagttcg atagcaaagc tctcaacgaa
     1141 acgttttcgc catgggttga ggagaagaac tggaaagacg ttctaatgag gttgagcgct
     1201 atgaaatttg cgttacaagc tgacagggac aaaattcctg gggttttatc agatctgagg
     1261 aaagactgtc ctttcagtgc ttttaagagg tttcctgaca aagagatgtg gtcgaaattg
     1321 acgaaggagg ctgtcattgc tttagctcaa attcaagctg cttcttcttt taagaggagg
     1381 gcagatgaaa agaatgccgt ttctgggttg attactgcaa cacctgctca agcttctact
     1441 agtaacgcga acccaagtgg ctcagcaacc acagtggtga gaccaccaag gttggacgac
     1501 tcgtcttttc aagaggatag cttttctttt ggtaaattcg atgacgcttc tactgcgtat
     1561 cataaagctc tctcctattt ggaaggttta aatttgaaac cgttatatag gagacagttt
     1621 gagaaatcgt acaacactag gtgggttcct gctgccgctc cggtggcgcc tgggccgcgt
     1681 ccgaatcctt aattctaagt ttgtcagata ctctttcaga gggtcacggt cgacggtacc
     1741 cccggggagc tcggtcaccg ggttgacgca ggtgcgtggg aagtaattgt ggatggtttg
     1801 tctgtattat tatcaatggt tttacttttg atgtgtataa ttgttgtggc cgtagccacc
     1861 ttgaaaagtg tcgtaaacgt tttgaagcaa gaaatcgaga aatctggaaa cagattgaac
     1921 gagttcgagc tgaaaaggca tttactacgg ttaaaaagtc tcgaaattcg aaaccctcta
     1981 agaaaatttt caaagagaga gaagattttg ggacaccaaa aagattttta agagatgatg
     2041 ttcctttcgg aattgatcag ttgtttgctt tttgatttta ttttaaattg ttatctgttt
     2101 ctgtgtatag actgtttgag attggcgttt ggccgactca ttgtcttacc ataggggaac
     2161 ggactttgtt tgtgttgtta ttttatttgt attttattaa aattctcaat gatctgaaaa
     2221 agctttgttg ctaagagatt attggggggt gagtaagtac ttttaaagtg atgatggtta
     2281 caaaggcaaa aggggttaaa cccctcgcct acgtaagcgt tattacgaag ctcgagtttc
     2341 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
     2401 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     2461 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt
     2521 aactagtgga tccggcgcgc cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
     2581 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
     2641 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
     2701 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
     2761 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
     2821 attgtaacga tgacagagcg ttgctgcctg tgatcgaatt ctaggtacta gaagaactcg
     2881 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     2941 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     3001 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     3061 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     3121 ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     3181 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     3241 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     3301 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     3361 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     3421 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     3481 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     3541 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     3601 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     3661 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
     3721 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
     3781 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
     3841 aaccacaatt atgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa
     3901 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
     3961 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
     4021 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
     4081 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
     4141 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
     4201 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
     4261 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
     4321 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
     4381 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
     4441 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
     4501 aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
     4561 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
     4621 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
     4681 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
     4741 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
     4801 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
     4861 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     4921 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     4981 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     5041 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     5101 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     5161 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     5221 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     5281 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     5341 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     5401 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     5461 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     5521 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     5581 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     5641 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     5701 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     5761 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     5821 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     5881 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     5941 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     6001 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
     6061 aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga
     6121 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
     6181 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca
     6241 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
     6301 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca
     6361 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
     6421 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
     6481 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat
     6541 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
     6601 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
     6661 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
     6721 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
     6781 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
     6841 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag
     6901 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
     6961 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag
     7021 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc
     7081 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
     7141 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc
     7201 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt
     7261 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac
     7321 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat
     7381 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt
     7441 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa
     7501 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg
     7561 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa
     7621 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt
     7681 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     7741 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     7801 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtacatgt gagcaaaagg
     7861 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7921 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7981 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8041 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8101 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8161 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8221 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8281 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8341 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8401 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8461 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8521 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8581 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8641 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8701 gatctgtcta tttcgttcat ccatagttgc ctgactctac ggatctgggg aaccctgtgg
     8761 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     8821 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     8881 tgatagttta aactgaaggc gggaaacgac aatctgatcc ggcgcgcc
//
