LctraKI vector (V016263)

LOCUS       62056_1400        8462 bp DNA     circular SYN 08-SEP-2021
DEFINITION  Cloning vector LctraKI, complete sequence.
ACCESSION   MZ783067
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Williamson ME, Yan Y, Scott MJ.
  TITLE     Conditional knockdown of transformer in sheep blowfly suggests a 
            role in repression of dosage compensation and potential for 
            population suppression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Williamson ME, Yan Y, Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2021) Entomology and Plant Pathology, North 
            Carolina State University, 112 Derieux Place, 1546 Thomas Hall, 
            Raleigh, NC 27607, USA
REFERENCE   3  (bases 1 to 8462)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2021) Entomology and Plant Pathology, North Carolina State 
            University, 112 Derieux Place, 1546 Thomas Hall, Raleigh, NC 27607, 
            USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..1012
                     /gene="Lctra"
                     /label=Lctra
                     /note="left homology arm"
     regulatory      1013..3284
                     /label=Lchsp83
                     /note="Lchsp83"
                     /regulatory_class="promoter"
     CDS             3285..3977
                     /label=ZsGreen
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
     regulatory      3990..4656
                     /label=p10 pA
                     /note="p10 pA"
                     /regulatory_class="polyA_signal_sequence"
     CDS             complement(4901..4915)
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     primer_bind     complement(5558..5574)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5604..5622)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5643..5659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5667..5683)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5691..5721)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5736..5757)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6045..6633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6807..7664)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7665..7769)
                     /label=AmpR promoter
     rep_origin      complement(7795..8250)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8392..8408
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8418..8436
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"