Basic Vector Information
- Vector Name:
- LctraKI
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8462 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Williamson ME, Yan Y, Scott MJ.
LctraKI vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
LctraKI vector Sequence
LOCUS 62056_1400 8462 bp DNA circular SYN 08-SEP-2021
DEFINITION Cloning vector LctraKI, complete sequence.
ACCESSION MZ783067
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8462)
AUTHORS Williamson ME, Yan Y, Scott MJ.
TITLE Conditional knockdown of transformer in sheep blowfly suggests a
role in repression of dosage compensation and potential for
population suppression
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8462)
AUTHORS Williamson ME, Yan Y, Scott MJ.
TITLE Direct Submission
JOURNAL Submitted (12-AUG-2021) Entomology and Plant Pathology, North
Carolina State University, 112 Derieux Place, 1546 Thomas Hall,
Raleigh, NC 27607, USA
REFERENCE 3 (bases 1 to 8462)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-AUG-2021) Entomology and Plant Pathology, North Carolina State
University, 112 Derieux Place, 1546 Thomas Hall, Raleigh, NC 27607,
USA"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..8462
/mol_type="other DNA"
/organism="synthetic DNA construct"
gene 1..1012
/gene="Lctra"
/label=Lctra
/note="left homology arm"
regulatory 1013..3284
/label=Lchsp83
/note="Lchsp83"
/regulatory_class="promoter"
CDS 3285..3977
/label=ZsGreen
/note="Zoanthus green fluorescent protein (Matz et al.,
1999)"
regulatory 3990..4656
/label=p10 pA
/note="p10 pA"
/regulatory_class="polyA_signal_sequence"
CDS complement(4901..4915)
/label=enterokinase site
/note="enterokinase recognition and cleavage site"
primer_bind complement(5558..5574)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(5604..5622)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5643..5659)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5667..5683)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5691..5721)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5736..5757)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6045..6633)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6807..7664)
/label=AmpR
/note="beta-lactamase"
promoter complement(7665..7769)
/label=AmpR promoter
rep_origin complement(7795..8250)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 8392..8408
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 8418..8436
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
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