LOCUS       62056_1400        8462 bp DNA     circular SYN 08-SEP-2021
DEFINITION  Cloning vector LctraKI, complete sequence.
ACCESSION   MZ783067
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Williamson ME, Yan Y, Scott MJ.
  TITLE     Conditional knockdown of transformer in sheep blowfly suggests a 
            role in repression of dosage compensation and potential for 
            population suppression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Williamson ME, Yan Y, Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2021) Entomology and Plant Pathology, North 
            Carolina State University, 112 Derieux Place, 1546 Thomas Hall, 
            Raleigh, NC 27607, USA
REFERENCE   3  (bases 1 to 8462)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2021) Entomology and Plant Pathology, North Carolina State 
            University, 112 Derieux Place, 1546 Thomas Hall, Raleigh, NC 27607, 
            USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..1012
                     /gene="Lctra"
                     /label=Lctra
                     /note="left homology arm"
     regulatory      1013..3284
                     /label=Lchsp83
                     /note="Lchsp83"
                     /regulatory_class="promoter"
     CDS             3285..3977
                     /label=ZsGreen
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
     regulatory      3990..4656
                     /label=p10 pA
                     /note="p10 pA"
                     /regulatory_class="polyA_signal_sequence"
     CDS             complement(4901..4915)
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     primer_bind     complement(5558..5574)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5604..5622)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5643..5659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5667..5683)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5691..5721)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5736..5757)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6045..6633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6807..7664)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7665..7769)
                     /label=AmpR promoter
     rep_origin      complement(7795..8250)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8392..8408
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8418..8436
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgaagtgct tcaaattcag gtgaaaaacc tatgaatttt acattagctt atcatcgaag
       61 ggatgttgtt cacttttggc aactctgaac tacatcttat cttagtttta gaagcaataa
      121 aaatccgata tttgaatgtc aaaaataaaa cgctttctct tcatgacact tatgaaaatt
      181 tcattggttt ttcaccaaat cttgatgtac ttaaaatatg ttatttgtaa tgacttttca
      241 acatctattt tcttactggg ttttcatttg taagaaaata tttggtttat aaaagtgaat
      301 agtatgtaat cgcattttta gatgtgatta agatgtagtt tgtaaagtat gacgacattt
      361 ttgctgggtt cttttaagaa tttcaatttt gatgaatttt gatatttgaa aacaaatttg
      421 gtctgtaaac atcggagata catctgttta aaatcccgaa aacttctgag cttcattatt
      481 ggtcatatct cctatacaac gccctccctc ttctaaagac cgcgtaatga tcatattggg
      541 ataacatttt tctgatcggt ctataactga tcatagctgc catatttaaa ccttcttctg
      601 aaaatctgca cataataatg ataacgggat aaaatttacg aataagtttt gcatattcaa
      661 agcatacagc taaatgtttt ttgcatcgga tcacaatttg tcatggctcc catataaaat
      721 agtaatttat aatatttgtt attatagcat agcccaggcc atgtatttag aaatatagtt
      781 ttacttggat ttgtacatat cgtataaaaa ataaacttga aaattcctta agatcgtgtg
      841 gccaaaaaaa tacacttggc cagtgtaaat atatataaag taatacgcaa acgaatacta
      901 acataaaatt taaactgatg cttaaggaat caagttcacc ggaaagatat cgtcatcggg
      961 acattaaaaa gcattcctcg ccgacgtctg gacgacgtaa gacaccggaa agcgaaggat
     1021 ctggttcata acatagaact ttttcgccct tctcgaaata acgagaacgg ggcccacgtg
     1081 ttgaaaccat tatcagaaaa taattcactt aaacattata atttgataaa aagaacacta
     1141 aaacaaaata ttataattat accgccaaaa attcaacaaa ctttaagaaa tttcgagtaa
     1201 attgcaccgc aaactaaaaa accgacatga taaacaaaaa aaatacataa aagagcgcaa
     1261 acagctgtca tttacagggt tgtagatatt tgaaattttt acctaaaaag gggaaaataa
     1321 aaggccacac ttttggaaat tacaaatttg tgagagaatt tttgatttca tttattaaat
     1381 ttatataaaa acaaaagagt tgttataata tgtatgagat aatgtttgca aattttaatt
     1441 tttaaatttt tacagtcaaa ttttataaaa tatgttaaaa gtatatgatt gtcacatttt
     1501 taaattattc cacttttcat tgtttttatt caagaccggc aactctgatg ttttgctgca
     1561 catcagctgc actggcgaaa aaattattaa tgcccccatg atgagagaaa atcaatattt
     1621 tttcatttta tgcattttta taaattttgt tagcaatatt attgttttaa taatgttatt
     1681 attttcttta ataaaacaat taaattactt tcatttataa tttatgatga ctaatatgta
     1741 aatcgaatgt gattaaatca tacatactat ttccatataa agcacaaagt ttaatatata
     1801 attaatatta aatttattta atttaaaaat taatagttaa tacatatttc aattaatcaa
     1861 atacatatta agcttgtaac ttagttcaaa aaacacttaa ttatacatag atttgtcaat
     1921 taataagact ttttaactat aatttaaaga ataaaacgtt tttgaaataa ataagttcat
     1981 tgtttgacta gaaaaatatt tagtttatgt catgatatta ctcagcaatg cctaagatta
     2041 attccttttt catttaatta ataactttta tatatttttg cataaataaa taaagtaaat
     2101 tatagttgat tcattccgca tgttattaaa aacagagaga acattgtaga aattttgaca
     2161 ttgatgcaat tttacagttt aaaagccact gcttctacat atatacaacg ctgcgtgggc
     2221 attaatcatg ttgaatgttt tgcacatttt tttgataatt tgcaactaat aaattaaaat
     2281 aacaataaaa acctataatt aatattggtt ttctacaatt ttaataaatt aattttttta
     2341 tatatctaaa atgtaaccaa tttcttttgt tatcaccaaa taaacaacca catgatgctg
     2401 tttagagcct acggaagtac gggtagtttg ttccagaaca ttctagtatt ttcgtttatt
     2461 tttgttttac ctataaattc agtgcatggc tgctaaccgg catcagtctt gaaagaaaaa
     2521 ttgtgcaaaa aacaccaaga attccaaacg ggagaaagtt aaaagtgttt taattttctt
     2581 aataaagaaa agtctgaaaa aagaacatta aaaaagtgtg tagttgttta atttcaattg
     2641 caaaaagaaa aataatttaa aaagtattta tttttgtgaa attaatataa aacaaacaca
     2701 tcaaggcaag tttttattaa aaaaaataaa atgtagtaac aaaacgtttt tgttcttttg
     2761 aaaaaagtgt gatgtaaaaa ctcaggtaat ttgccagaaa gaaaaacctt caatatggtt
     2821 taagaataac gttactaagt aattgtaaat tgttctagat taaattttct ttggccatga
     2881 catttttaga aaataaatct agtatataaa gaaaatttca ccatgctaat aaaaagaaat
     2941 ttctagcatg cgatttttta aagaaaattt ttcaaaattt tttcgaaatt ataatcgata
     3001 tttcatgttt tcattcgtta aaaagcatac ccaatccacc cctaaaaaac tagtaattct
     3061 taacttaaag agatttctag atttttatta aaaattttca gtaccaatcc cactccctac
     3121 acttaacgaa aaacgtgcgt cattaaaatt atttatactt catattttga ttgattattt
     3181 tactaataat ttgtattttt ctattttttt tttattaaac acagatgcct gaagaagttg
     3241 aaacttttgc tttccaagct gaaattgctc agcttaaagg atccatggct cagtcaaagc
     3301 acggtctaac aaaagaaatg acaatgaaat accgtatgga agggtgcgtc gatggacata
     3361 aatttgtgat cacgggagag ggcattggat atccgttcaa agggaaacag gctattaatc
     3421 tgtgtgtggt cgaaggtgga ccattgccat ttgccgaaga catattgtca gctgccttta
     3481 tgtacggaaa cagggttttc actgaatatc ctcaagacat agctgactat ttcaagaact
     3541 cgtgtcctgc tggttataca tgggacaggt cttttctctt tgaggatgga gcagtttgca
     3601 tatgtaatgc agatataaca gtgagtgttg aagaaaactg catgtatcat gagtccaaat
     3661 tttatggagt gaattttcct gctgatggac ctgtgatgaa aaagatgaca gataactggg
     3721 agccatcctg cgagaagatc ataccagtac ctaagcaggg gatattgaaa ggggatgtct
     3781 ccatgtacct ccttctgaag gatggtgggc gtttacggtg ccaattcgac acagtttaca
     3841 aagcaaagtc tgtgccaaga aagatgccgg actggcactt catccagcat aagctcaccc
     3901 gtgaagaccg cagcgatgct aagaatcaga aatggcatct gacagaacat gctattgcat
     3961 ccggatctgc attgccctga agcttctaga atgaatcgtt tttaaaataa caaatcaatt
     4021 gttttataat attcgtacga ttctttgatt atgtaataaa atgtgatcat taggaagatt
     4081 acgaaaaata taaaaaatat gagttctgtg tgtataacaa atgctgtaaa cgccacaatt
     4141 gtgtttgttg caaataaacc catgattatt tgattaaaat tgttgttttc tttgttcata
     4201 gacaatagtg tgttttgcct aaacgtgtac tgcataaact ccatgcgagt gtatagcgag
     4261 ctagtggcta acgcttgccc caccaaagta gattcgtcaa aatcctcaat ttcatcaccc
     4321 tcctccaagt ttaacatttg gccgtcggaa ttaacttcta aagatgccac ataatctaat
     4381 aaatgaaata gagattcaaa cgtggcgtca tcgtccgttt cgaccatttc cgaaaagaac
     4441 tcgggcataa actctatgat ttctctggac gtggtgttgt cgaaactctc aaagtacgca
     4501 gtcaggaacg tgcgcgacat gtcgtcggga aactcgcgcg gaaacatgtt gttgtaaccg
     4561 aacgggtccc atagcgccaa aaccaaatct gccagcgtca atagaatgag cacgatgccg
     4621 acaatggagc tggcttggat agcgattcga gttaacctgg gcgcagtgaa agaccaacac
     4681 acagccatga taaacataat tataatgtaa aatctaacaa tacaatgact gataaatata
     4741 aaaggagtcg tagaagtaaa tcacgttcac cgccacataa cgccaataag aagactgtcg
     4801 aaaaagttcc ttactatcgt gatgaacaac gtgaaaaaga tcgtataaga cgattatatg
     4861 gacgttcacg ttctcgtaca ccaccgccac cagtagcaaa cttatcgtca tcatcgtcgt
     4921 caactgcaaa gcgacgtatt gaaagtccat cgagacggcg aagacgttca accagtagag
     4981 atcgtcatcg tagacaatca ccatacattc accgaagcgt aagaagagac tacagaagcc
     5041 gtcgttctag aacacgttca cgaactcgtt ctccacaaag acgtgaacgt cacaaacatt
     5101 ctagtagatc tagcagagag cgtgacaatg agcacaaaga agatgtaaat tccttaacaa
     5161 ctgctattat acccgcaaca cctcagatta ttcctatacc agtacctgtg cccgctgaat
     5221 atgctgctgc ctatacattc gtatgttcat ttgaattgta caattgttaa tatctcaaca
     5281 tatttacgat ttatttcatt tacagcccgg ctggacagct ccacaaccaa cttggccacc
     5341 gtctcaccat cggccaccag ccacatcaca ttttgctccc tttcccatgt ggcctatgat
     5401 gcctcctctt agaccaccac ctcatcaagc gtcttatggt ggcttaccac caccaacatt
     5461 agcttatcca ccaatgacag cttcatatcg tcctcatgga ccgcaaagat atcctcaacc
     5521 aagacacaat agcaacaaca actatcaaac tcggtatcga taccgtcgac ctcgaggggg
     5581 ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     5641 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5701 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5761 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5821 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5881 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5941 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6001 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6061 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6121 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6181 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6241 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6301 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6361 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6421 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6481 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6541 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6601 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6661 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6721 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6781 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6841 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6901 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6961 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7021 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7081 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7141 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7201 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7261 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7321 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7381 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7441 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7501 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7561 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7621 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7681 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7741 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     7801 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     7861 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     7921 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     7981 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     8041 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     8101 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     8161 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     8221 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     8281 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     8341 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     8401 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg
     8461 gc
//
